PubMed:28624519 / 185-1606 JSONTXT

{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T1","span":{"begin":0,"end":1421},"obj":"None"}],"text":"Bombyx mori nucleopolyhedrovirus (BmNPV) has been identified as a major pathogen responsible for severe economic loss. Most silkworm strains are susceptible to BmNPV, with only a few highly resistant strains thus far identified. Here we investigated the molecular basis of silkworm resistance to BmNPV using susceptible (the recurrent parent P50) and resistant (near-isogenic line BC9) strains and a combination of iTRAQ-based quantitative proteomics, reverse-transcription quantitative PCR and Western blotting. By comparing the proteomes of infected and non-infected P50 and BC9 silkworms, we identified 793 differentially expressed proteins (DEPs). By gene ontology and KEGG enrichment analyses, we found that these DEPs are preferentially involved in metabolism, catalytic activity, amino sugar and nucleotide sugar metabolism and carbon metabolism. 114 (14.38%) DEPs were associated with the cytoskeleton, immune response, apoptosis, ubiquitination, translation, ion transport, endocytosis and endopeptidase activity. After removing the genetic background and individual immune stress response proteins, we identified 84 DEPs were found that are potentially involved in resistance to BmNPV. Further studies showed that a serine protease was down-regulated in P50 and up-regulated in BC9 after BmNPV infection. Taken together, these results provide insights into the molecular mechanism of silkworm response to BmNPV."}

{"project":"sentences","denotations":[{"id":"T2","span":{"begin":0,"end":118},"obj":"Sentence"},{"id":"T3","span":{"begin":119,"end":228},"obj":"Sentence"},{"id":"T4","span":{"begin":229,"end":512},"obj":"Sentence"},{"id":"T5","span":{"begin":513,"end":651},"obj":"Sentence"},{"id":"T6","span":{"begin":652,"end":853},"obj":"Sentence"},{"id":"T7","span":{"begin":854,"end":1022},"obj":"Sentence"},{"id":"T8","span":{"begin":1023,"end":1195},"obj":"Sentence"},{"id":"T9","span":{"begin":1196,"end":1314},"obj":"Sentence"},{"id":"T10","span":{"begin":1315,"end":1421},"obj":"Sentence"},{"id":"T2","span":{"begin":0,"end":118},"obj":"Sentence"},{"id":"T3","span":{"begin":119,"end":228},"obj":"Sentence"},{"id":"T4","span":{"begin":229,"end":512},"obj":"Sentence"},{"id":"T5","span":{"begin":513,"end":651},"obj":"Sentence"},{"id":"T6","span":{"begin":652,"end":853},"obj":"Sentence"},{"id":"T7","span":{"begin":854,"end":1022},"obj":"Sentence"},{"id":"T8","span":{"begin":1023,"end":1195},"obj":"Sentence"},{"id":"T9","span":{"begin":1196,"end":1314},"obj":"Sentence"},{"id":"T10","span":{"begin":1315,"end":1421},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Bombyx mori nucleopolyhedrovirus (BmNPV) has been identified as a major pathogen responsible for severe economic loss. Most silkworm strains are susceptible to BmNPV, with only a few highly resistant strains thus far identified. Here we investigated the molecular basis of silkworm resistance to BmNPV using susceptible (the recurrent parent P50) and resistant (near-isogenic line BC9) strains and a combination of iTRAQ-based quantitative proteomics, reverse-transcription quantitative PCR and Western blotting. By comparing the proteomes of infected and non-infected P50 and BC9 silkworms, we identified 793 differentially expressed proteins (DEPs). By gene ontology and KEGG enrichment analyses, we found that these DEPs are preferentially involved in metabolism, catalytic activity, amino sugar and nucleotide sugar metabolism and carbon metabolism. 114 (14.38%) DEPs were associated with the cytoskeleton, immune response, apoptosis, ubiquitination, translation, ion transport, endocytosis and endopeptidase activity. After removing the genetic background and individual immune stress response proteins, we identified 84 DEPs were found that are potentially involved in resistance to BmNPV. Further studies showed that a serine protease was down-regulated in P50 and up-regulated in BC9 after BmNPV infection. Taken together, these results provide insights into the molecular mechanism of silkworm response to BmNPV."}

{"project":"silkworm","denotations":[{"id":"T3","span":{"begin":0,"end":32},"obj":"Species:271108"},{"id":"T4","span":{"begin":34,"end":39},"obj":"Species:271108"},{"id":"T5","span":{"begin":124,"end":132},"obj":"Species:7091"},{"id":"T6","span":{"begin":160,"end":165},"obj":"Species:271108"},{"id":"T7","span":{"begin":273,"end":281},"obj":"Species:7091"},{"id":"T8","span":{"begin":296,"end":301},"obj":"Species:271108"},{"id":"T9","span":{"begin":581,"end":590},"obj":"Species:7091"},{"id":"T10","span":{"begin":787,"end":798},"obj":"Chemical:MESH:D000606"},{"id":"T11","span":{"begin":803,"end":819},"obj":"Chemical:-"},{"id":"T12","span":{"begin":835,"end":841},"obj":"Chemical:MESH:D002244"},{"id":"T13","span":{"begin":1189,"end":1194},"obj":"Species:271108"},{"id":"T14","span":{"begin":1298,"end":1303},"obj":"Species:271108"},{"id":"T15","span":{"begin":1304,"end":1313},"obj":"Disease:MESH:D007239"},{"id":"T16","span":{"begin":1394,"end":1402},"obj":"Species:7091"},{"id":"T17","span":{"begin":1415,"end":1420},"obj":"Species:271108"}],"text":"Bombyx mori nucleopolyhedrovirus (BmNPV) has been identified as a major pathogen responsible for severe economic loss. Most silkworm strains are susceptible to BmNPV, with only a few highly resistant strains thus far identified. Here we investigated the molecular basis of silkworm resistance to BmNPV using susceptible (the recurrent parent P50) and resistant (near-isogenic line BC9) strains and a combination of iTRAQ-based quantitative proteomics, reverse-transcription quantitative PCR and Western blotting. By comparing the proteomes of infected and non-infected P50 and BC9 silkworms, we identified 793 differentially expressed proteins (DEPs). By gene ontology and KEGG enrichment analyses, we found that these DEPs are preferentially involved in metabolism, catalytic activity, amino sugar and nucleotide sugar metabolism and carbon metabolism. 114 (14.38%) DEPs were associated with the cytoskeleton, immune response, apoptosis, ubiquitination, translation, ion transport, endocytosis and endopeptidase activity. After removing the genetic background and individual immune stress response proteins, we identified 84 DEPs were found that are potentially involved in resistance to BmNPV. Further studies showed that a serine protease was down-regulated in P50 and up-regulated in BC9 after BmNPV infection. Taken together, these results provide insights into the molecular mechanism of silkworm response to BmNPV."}

{"project":"silkwormbase","denotations":[{"id":"T10","span":{"begin":787,"end":798},"obj":"Chemical:MESH:D000606"},{"id":"T11","span":{"begin":803,"end":819},"obj":"Chemical:-"},{"id":"T12","span":{"begin":835,"end":841},"obj":"Chemical:MESH:D002244"},{"id":"T13","span":{"begin":1189,"end":1194},"obj":"Species:271108"},{"id":"T14","span":{"begin":1298,"end":1303},"obj":"Species:271108"},{"id":"T15","span":{"begin":1304,"end":1313},"obj":"Disease:MESH:D007239"},{"id":"T16","span":{"begin":1394,"end":1402},"obj":"Species:7091"},{"id":"T17","span":{"begin":1415,"end":1420},"obj":"Species:271108"},{"id":"T3","span":{"begin":0,"end":32},"obj":"Species:271108"},{"id":"T4","span":{"begin":34,"end":39},"obj":"Species:271108"},{"id":"T5","span":{"begin":124,"end":132},"obj":"Species:7091"},{"id":"T6","span":{"begin":160,"end":165},"obj":"Species:271108"},{"id":"T7","span":{"begin":273,"end":281},"obj":"Species:7091"},{"id":"T8","span":{"begin":296,"end":301},"obj":"Species:271108"},{"id":"T9","span":{"begin":581,"end":590},"obj":"Species:7091"}],"text":"Bombyx mori nucleopolyhedrovirus (BmNPV) has been identified as a major pathogen responsible for severe economic loss. Most silkworm strains are susceptible to BmNPV, with only a few highly resistant strains thus far identified. Here we investigated the molecular basis of silkworm resistance to BmNPV using susceptible (the recurrent parent P50) and resistant (near-isogenic line BC9) strains and a combination of iTRAQ-based quantitative proteomics, reverse-transcription quantitative PCR and Western blotting. By comparing the proteomes of infected and non-infected P50 and BC9 silkworms, we identified 793 differentially expressed proteins (DEPs). By gene ontology and KEGG enrichment analyses, we found that these DEPs are preferentially involved in metabolism, catalytic activity, amino sugar and nucleotide sugar metabolism and carbon metabolism. 114 (14.38%) DEPs were associated with the cytoskeleton, immune response, apoptosis, ubiquitination, translation, ion transport, endocytosis and endopeptidase activity. After removing the genetic background and individual immune stress response proteins, we identified 84 DEPs were found that are potentially involved in resistance to BmNPV. Further studies showed that a serine protease was down-regulated in P50 and up-regulated in BC9 after BmNPV infection. Taken together, these results provide insights into the molecular mechanism of silkworm response to BmNPV."}

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":1304,"end":1313},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"}],"text":"Bombyx mori nucleopolyhedrovirus (BmNPV) has been identified as a major pathogen responsible for severe economic loss. Most silkworm strains are susceptible to BmNPV, with only a few highly resistant strains thus far identified. Here we investigated the molecular basis of silkworm resistance to BmNPV using susceptible (the recurrent parent P50) and resistant (near-isogenic line BC9) strains and a combination of iTRAQ-based quantitative proteomics, reverse-transcription quantitative PCR and Western blotting. By comparing the proteomes of infected and non-infected P50 and BC9 silkworms, we identified 793 differentially expressed proteins (DEPs). By gene ontology and KEGG enrichment analyses, we found that these DEPs are preferentially involved in metabolism, catalytic activity, amino sugar and nucleotide sugar metabolism and carbon metabolism. 114 (14.38%) DEPs were associated with the cytoskeleton, immune response, apoptosis, ubiquitination, translation, ion transport, endocytosis and endopeptidase activity. After removing the genetic background and individual immune stress response proteins, we identified 84 DEPs were found that are potentially involved in resistance to BmNPV. Further studies showed that a serine protease was down-regulated in P50 and up-regulated in BC9 after BmNPV infection. Taken together, these results provide insights into the molecular mechanism of silkworm response to BmNPV."}

{"project":"NCBITAXON","denotations":[{"id":"T4","span":{"begin":0,"end":32},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":34,"end":39},"obj":"OrganismTaxon"},{"id":"T6","span":{"begin":124,"end":132},"obj":"OrganismTaxon"},{"id":"T7","span":{"begin":160,"end":165},"obj":"OrganismTaxon"},{"id":"T8","span":{"begin":273,"end":281},"obj":"OrganismTaxon"},{"id":"T9","span":{"begin":296,"end":301},"obj":"OrganismTaxon"},{"id":"T10","span":{"begin":581,"end":590},"obj":"OrganismTaxon"},{"id":"T11","span":{"begin":1189,"end":1194},"obj":"OrganismTaxon"},{"id":"T12","span":{"begin":1298,"end":1303},"obj":"OrganismTaxon"},{"id":"T13","span":{"begin":1394,"end":1402},"obj":"OrganismTaxon"},{"id":"T14","span":{"begin":1415,"end":1420},"obj":"OrganismTaxon"}],"attributes":[{"id":"A4","pred":"db_id","subj":"T4","obj":"271108"},{"id":"A5","pred":"db_id","subj":"T5","obj":"271108"},{"id":"A6","pred":"db_id","subj":"T6","obj":"7091"},{"id":"A7","pred":"db_id","subj":"T7","obj":"271108"},{"id":"A8","pred":"db_id","subj":"T8","obj":"7091"},{"id":"A9","pred":"db_id","subj":"T9","obj":"271108"},{"id":"A10","pred":"db_id","subj":"T10","obj":"7091"},{"id":"A11","pred":"db_id","subj":"T11","obj":"271108"},{"id":"A12","pred":"db_id","subj":"T12","obj":"271108"},{"id":"A13","pred":"db_id","subj":"T13","obj":"7091"},{"id":"A14","pred":"db_id","subj":"T14","obj":"271108"}],"text":"Bombyx mori nucleopolyhedrovirus (BmNPV) has been identified as a major pathogen responsible for severe economic loss. Most silkworm strains are susceptible to BmNPV, with only a few highly resistant strains thus far identified. Here we investigated the molecular basis of silkworm resistance to BmNPV using susceptible (the recurrent parent P50) and resistant (near-isogenic line BC9) strains and a combination of iTRAQ-based quantitative proteomics, reverse-transcription quantitative PCR and Western blotting. By comparing the proteomes of infected and non-infected P50 and BC9 silkworms, we identified 793 differentially expressed proteins (DEPs). By gene ontology and KEGG enrichment analyses, we found that these DEPs are preferentially involved in metabolism, catalytic activity, amino sugar and nucleotide sugar metabolism and carbon metabolism. 114 (14.38%) DEPs were associated with the cytoskeleton, immune response, apoptosis, ubiquitination, translation, ion transport, endocytosis and endopeptidase activity. After removing the genetic background and individual immune stress response proteins, we identified 84 DEPs were found that are potentially involved in resistance to BmNPV. Further studies showed that a serine protease was down-regulated in P50 and up-regulated in BC9 after BmNPV infection. Taken together, these results provide insights into the molecular mechanism of silkworm response to BmNPV."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T2","span":{"begin":897,"end":909},"obj":"Body_part"}],"attributes":[{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/GO_0005856"}],"text":"Bombyx mori nucleopolyhedrovirus (BmNPV) has been identified as a major pathogen responsible for severe economic loss. Most silkworm strains are susceptible to BmNPV, with only a few highly resistant strains thus far identified. Here we investigated the molecular basis of silkworm resistance to BmNPV using susceptible (the recurrent parent P50) and resistant (near-isogenic line BC9) strains and a combination of iTRAQ-based quantitative proteomics, reverse-transcription quantitative PCR and Western blotting. By comparing the proteomes of infected and non-infected P50 and BC9 silkworms, we identified 793 differentially expressed proteins (DEPs). By gene ontology and KEGG enrichment analyses, we found that these DEPs are preferentially involved in metabolism, catalytic activity, amino sugar and nucleotide sugar metabolism and carbon metabolism. 114 (14.38%) DEPs were associated with the cytoskeleton, immune response, apoptosis, ubiquitination, translation, ion transport, endocytosis and endopeptidase activity. After removing the genetic background and individual immune stress response proteins, we identified 84 DEPs were found that are potentially involved in resistance to BmNPV. Further studies showed that a serine protease was down-regulated in P50 and up-regulated in BC9 after BmNPV infection. Taken together, these results provide insights into the molecular mechanism of silkworm response to BmNPV."}