PubMed:28618068 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/28618068","sourcedb":"PubMed","sourceid":"28618068","text":"Molecular cloning and analysis of a C-type lectin from silkworm Bombyx mori.\nC-type lectins (CTLs) play a variety of roles in plants and animals. They are involved in animal development, pathogen recognition, and the activation of immune responses. CTLs carry one or more non-catalytic carbohydrate-recognition domains (CRDs) to bind specific carbohydrates reversibly. Here, we report the molecular cloning and functional analysis of a single-CRD CTL, named C-type lectin-S2 (BmCTL-S2) from the domesticated silkmoth Bombyx mori (Lepidoptera: Bombycidae). The ORF of CTL-S2 is 666 bp, which encodes a putative protein of 221 amino acids. BmCTL-S2 is expressed in a variety of immune-related tissues, including hemocytes and fat body among others. BmCTL-S2 mRNA level in the midgut and the fat body was significantly increased by bacterial challenges. The recombinant protein (rBmCTL-S2) bound different bacterial cell wall components and bacterial cells. rBmCTL-S2 also inhibited the growth of Bacillus subtilis and Staphylococcus aureus. Taken together, we infer that BmCTL-S2 is a pattern-recognition receptor with antibacterial activities.","tracks":[{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":55,"end":63},"obj":"Species:7091"},{"id":"T2","span":{"begin":64,"end":75},"obj":"Species:7091"},{"id":"T3","span":{"begin":286,"end":298},"obj":"Chemical:MESH:D002241"},{"id":"T4","span":{"begin":343,"end":356},"obj":"Chemical:MESH:D002241"},{"id":"T5","span":{"begin":517,"end":528},"obj":"Species:7091"},{"id":"T6","span":{"begin":955,"end":964},"obj":"Chemical:-"},{"id":"T7","span":{"begin":994,"end":1011},"obj":"Species:1423"},{"id":"T8","span":{"begin":1016,"end":1037},"obj":"Species:1280"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkworm"},{"subj":"T2","pred":"source","obj":"silkworm"},{"subj":"T3","pred":"source","obj":"silkworm"},{"subj":"T4","pred":"source","obj":"silkworm"},{"subj":"T5","pred":"source","obj":"silkworm"},{"subj":"T6","pred":"source","obj":"silkworm"},{"subj":"T7","pred":"source","obj":"silkworm"},{"subj":"T8","pred":"source","obj":"silkworm"}]},{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":55,"end":63},"obj":"Species:7091"},{"id":"T2","span":{"begin":64,"end":75},"obj":"Species:7091"},{"id":"T3","span":{"begin":286,"end":298},"obj":"Chemical:MESH:D002241"},{"id":"T4","span":{"begin":343,"end":356},"obj":"Chemical:MESH:D002241"},{"id":"T5","span":{"begin":517,"end":528},"obj":"Species:7091"},{"id":"T6","span":{"begin":955,"end":964},"obj":"Chemical:-"},{"id":"T7","span":{"begin":994,"end":1011},"obj":"Species:1423"},{"id":"T8","span":{"begin":1016,"end":1037},"obj":"Species:1280"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkwormbase"},{"subj":"T2","pred":"source","obj":"silkwormbase"},{"subj":"T3","pred":"source","obj":"silkwormbase"},{"subj":"T4","pred":"source","obj":"silkwormbase"},{"subj":"T5","pred":"source","obj":"silkwormbase"},{"subj":"T6","pred":"source","obj":"silkwormbase"},{"subj":"T7","pred":"source","obj":"silkwormbase"},{"subj":"T8","pred":"source","obj":"silkwormbase"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"silkworm","color":"#ece093","default":true},{"id":"silkwormbase","color":"#c693ec"}]}]}}