PubMed:28450392
Annnotations
GlyCosmos15-Species
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| 19 | 145-150 | Species | denotes | human | 9606 |
| 24 | 816-821 | Species | denotes | human | 9606 |
GlyCosmos6-Glycan-Motif-Image
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 9-28 | Glycan_Motif | denotes | N-acetylglucosamine | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G64581RP |
| T2 | 300-319 | Glycan_Motif | denotes | N-acetylglucosamine | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G64581RP |
| T3 | 321-329 | Glycan_Motif | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G89565QL|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G49108TO |
| T5 | 383-391 | Glycan_Motif | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G89565QL|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G49108TO |
| T7 | 1099-1107 | Glycan_Motif | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G89565QL|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G49108TO |
| T9 | 1278-1286 | Glycan_Motif | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G89565QL|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G49108TO |
| T11 | 1500-1508 | Glycan_Motif | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G89565QL|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G49108TO |
GlyCosmos6-Glycan-Motif-Structure
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 9-28 | https://glytoucan.org/Structures/Glycans/G64581RP | denotes | N-acetylglucosamine |
| T2 | 300-319 | https://glytoucan.org/Structures/Glycans/G64581RP | denotes | N-acetylglucosamine |
| T3 | 321-329 | https://glytoucan.org/Structures/Glycans/G49108TO | denotes | O-GlcNAc |
| T4 | 321-329 | https://glytoucan.org/Structures/Glycans/G89565QL | denotes | O-GlcNAc |
| T5 | 383-391 | https://glytoucan.org/Structures/Glycans/G49108TO | denotes | O-GlcNAc |
| T6 | 383-391 | https://glytoucan.org/Structures/Glycans/G89565QL | denotes | O-GlcNAc |
| T7 | 1099-1107 | https://glytoucan.org/Structures/Glycans/G49108TO | denotes | O-GlcNAc |
| T8 | 1099-1107 | https://glytoucan.org/Structures/Glycans/G89565QL | denotes | O-GlcNAc |
| T9 | 1278-1286 | https://glytoucan.org/Structures/Glycans/G49108TO | denotes | O-GlcNAc |
| T10 | 1278-1286 | https://glytoucan.org/Structures/Glycans/G89565QL | denotes | O-GlcNAc |
| T11 | 1500-1508 | https://glytoucan.org/Structures/Glycans/G49108TO | denotes | O-GlcNAc |
| T12 | 1500-1508 | https://glytoucan.org/Structures/Glycans/G89565QL | denotes | O-GlcNAc |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-140 | Sentence | denotes | O-Linked N-acetylglucosamine transferase 1 regulates global histone H4 acetylation via stabilization of the nonspecific lethal protein NSL3. |
| T2 | 141-361 | Sentence | denotes | The human males absent on the first (MOF)-containing histone acetyltransferase nonspecific lethal (NSL) complex comprises nine subunits including the O-linked N-acetylglucosamine (O-GlcNAc) transferase, isoform 1 (OGT1). |
| T3 | 362-573 | Sentence | denotes | However, whether the O-GlcNAc transferase activity of OGT1 controls histone acetyltransferase activity of the NSL complex and whether OGT1 physically interacts with the other NSL complex subunits remain unclear. |
| T4 | 574-775 | Sentence | denotes | Here, we demonstrate that OGT1 regulates the activity of the NSL complex by mainly acetylating histone H4 Lys-16, Lys-5, and Lys-8 via O-GlcNAcylation and stabilization of the NSL complex subunit NSL3. |
| T5 | 776-919 | Sentence | denotes | Knocking down or overexpressing OGT1 in human cells remarkably affected the global acetylation of histone H4 residues Lys-16, Lys-5, and Lys-8. |
| T6 | 920-1024 | Sentence | denotes | Because OGT1 is a subunit of the NSL complex, we also investigated the function of OGT1 in this complex. |
| T7 | 1025-1193 | Sentence | denotes | Co-transfection/co-immunoprecipitation experiments combined with in vitro O-GlcNAc transferase assays confirmed that OGT1 specifically binds to and O-GlcNAcylates NSL3. |
| T8 | 1194-1317 | Sentence | denotes | In addition, wheat germ agglutinin affinity purification verified the occurrence of O-GlcNAc modification on NSL3 in cells. |
| T9 | 1318-1396 | Sentence | denotes | Moreover, O-GlcNAcylation of NSL3 by wild-type OGT1 (OGT1-WT) stabilized NSL3. |
| T10 | 1397-1530 | Sentence | denotes | This stabilization was lost after co-transfection of NSL3 with an OGT1 mutant, OGT1(C964A), that lacks O-GlcNAc transferase activity. |
| T11 | 1531-1671 | Sentence | denotes | Furthermore, stabilization of NSL3 by OGT1-WT significantly increased the global acetylation levels of H4 Lys-5, Lys-8, and Lys-16 in cells. |
| T12 | 1672-1766 | Sentence | denotes | These results suggest that OGT1 regulates the activity of the NSL complex by stabilizing NSL3. |
Glycosmos6-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glyco_epitope_db_id |
|---|---|---|---|---|---|
| T1 | 321-329 | GlycoEpitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T2 | 383-391 | GlycoEpitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T3 | 1099-1107 | GlycoEpitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T4 | 1278-1286 | GlycoEpitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T5 | 1500-1508 | GlycoEpitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
Glycosmos6-MAT
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 151-156 | http://purl.obolibrary.org/obo/MAT_0000029 | denotes | males |
NGLY1-deficiency
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| PD-NGLY1-deficiency-B_T1 | 9-28 | chem:24139 | denotes | N-acetylglucosamine |
| PD-NGLY1-deficiency-B_T2 | 300-319 | chem:24139 | denotes | N-acetylglucosamine |
| PD-NGLY1-deficiency-B_T3 | 323-329 | chem:24139 | denotes | GlcNAc |
| PD-NGLY1-deficiency-B_T4 | 385-391 | chem:24139 | denotes | GlcNAc |
| PD-NGLY1-deficiency-B_T5 | 1101-1107 | chem:24139 | denotes | GlcNAc |
| PD-NGLY1-deficiency-B_T6 | 1280-1286 | chem:24139 | denotes | GlcNAc |
| PD-NGLY1-deficiency-B_T7 | 1502-1508 | chem:24139 | denotes | GlcNAc |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 321-329 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
| T2 | 383-391 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
| T3 | 1099-1107 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
| T4 | 1278-1286 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
| T5 | 1500-1508 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
Anatomy-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 151-156 | Body_part | denotes | males | http://purl.obolibrary.org/obo/MAT_0000029 |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 321-329 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
| T2 | 383-391 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
| T3 | 1099-1107 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
| T4 | 1278-1286 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
| T5 | 1500-1508 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-140 | Sentence | denotes | O-Linked N-acetylglucosamine transferase 1 regulates global histone H4 acetylation via stabilization of the nonspecific lethal protein NSL3. |
| T2 | 141-361 | Sentence | denotes | The human males absent on the first (MOF)-containing histone acetyltransferase nonspecific lethal (NSL) complex comprises nine subunits including the O-linked N-acetylglucosamine (O-GlcNAc) transferase, isoform 1 (OGT1). |
| T3 | 362-573 | Sentence | denotes | However, whether the O-GlcNAc transferase activity of OGT1 controls histone acetyltransferase activity of the NSL complex and whether OGT1 physically interacts with the other NSL complex subunits remain unclear. |
| T4 | 574-775 | Sentence | denotes | Here, we demonstrate that OGT1 regulates the activity of the NSL complex by mainly acetylating histone H4 Lys-16, Lys-5, and Lys-8 via O-GlcNAcylation and stabilization of the NSL complex subunit NSL3. |
| T5 | 776-919 | Sentence | denotes | Knocking down or overexpressing OGT1 in human cells remarkably affected the global acetylation of histone H4 residues Lys-16, Lys-5, and Lys-8. |
| T6 | 920-1024 | Sentence | denotes | Because OGT1 is a subunit of the NSL complex, we also investigated the function of OGT1 in this complex. |
| T7 | 1025-1193 | Sentence | denotes | Co-transfection/co-immunoprecipitation experiments combined with in vitro O-GlcNAc transferase assays confirmed that OGT1 specifically binds to and O-GlcNAcylates NSL3. |
| T8 | 1194-1317 | Sentence | denotes | In addition, wheat germ agglutinin affinity purification verified the occurrence of O-GlcNAc modification on NSL3 in cells. |
| T9 | 1318-1396 | Sentence | denotes | Moreover, O-GlcNAcylation of NSL3 by wild-type OGT1 (OGT1-WT) stabilized NSL3. |
| T10 | 1397-1530 | Sentence | denotes | This stabilization was lost after co-transfection of NSL3 with an OGT1 mutant, OGT1(C964A), that lacks O-GlcNAc transferase activity. |
| T11 | 1531-1671 | Sentence | denotes | Furthermore, stabilization of NSL3 by OGT1-WT significantly increased the global acetylation levels of H4 Lys-5, Lys-8, and Lys-16 in cells. |
| T12 | 1672-1766 | Sentence | denotes | These results suggest that OGT1 regulates the activity of the NSL complex by stabilizing NSL3. |
GlyCosmos15-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 321-329 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T2 | 383-391 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T3 | 1099-1107 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T4 | 1278-1286 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T5 | 1500-1508 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
GlyCosmos15-NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 145-150 | OrganismTaxon | denotes | human | 9606 |
| T2 | 816-821 | OrganismTaxon | denotes | human | 9606 |
| T3 | 1207-1212 | OrganismTaxon | denotes | wheat | 4565 |
GlyCosmos15-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 151-156 | Body_part | denotes | males | http://purl.obolibrary.org/obo/MAT_0000029 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 145-150 | OrganismTaxon | denotes | human | 9606 |
| T2 | 816-821 | OrganismTaxon | denotes | human | 9606 |
| T3 | 1207-1212 | OrganismTaxon | denotes | wheat | 4565 |
GlyCosmos-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 321-329 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T2 | 383-391 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T3 | 1099-1107 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T4 | 1278-1286 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T5 | 1500-1508 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
GlyCosmos15-Lectin
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 1207-1228 | GL_000042 | denotes | wheat germ agglutinin |
Lectin_test
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 1207-1228 | GL_000042 | denotes | wheat germ agglutinin |