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PubMed:2842341 JSONTXT

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GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 1385-1392 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO

sentences

Id Subject Object Predicate Lexical cue
T1 0-77 Sentence denotes Characterization of insulin-stimulated microtubule-associated protein kinase.
T2 78-165 Sentence denotes Rapid isolation and stabilization of a novel serine/threonine kinase from 3T3-L1 cells.
T3 166-445 Sentence denotes A protein kinase, termed microtubule-associated protein (MAP) kinase, which phosphorylates microtubule-associated protein 2 (MAP-2) in vitro and is stimulated 1.5-3-fold in extracts from insulin-treated 3T3-L1 cells has been identified (Ray, L.B., and Sturgill, T.W. (1987) Proc.
T4 446-451 Sentence denotes Natl.
T5 452-457 Sentence denotes Acad.
T6 458-462 Sentence denotes Sci.
T7 463-469 Sentence denotes U.S.A.
T8 470-485 Sentence denotes 84, 1502-1506).
T9 486-619 Sentence denotes Here, we describe chromatographic properties of MAP kinase and provide biochemical characterization of the partially purified enzyme.
T10 620-742 Sentence denotes Isolation of the enzyme is facilitated by its unusually high affinity for hydrophobic interaction chromatography matrices.
T11 743-905 Sentence denotes The molecular weight of the partially purified enzyme was determined to be 35,000 by gel filtration chromatography and 37,000 by glycerol gradient centrifugation.
T12 906-1091 Sentence denotes MAP kinase activity of chromatographic fractions correlated precisely with the presence of a 40-kDa phosphoprotein detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
T13 1092-1157 Sentence denotes MAP kinase has a Km of 7 microM for ATP and does not utilize GTP.
T14 1158-1309 Sentence denotes Acetyl-CoA carboxylase, ATP citrate-lyase, casein, histones, phosvitin, protamine, and ribosomal protein S6 were all poor substrates relative to MAP-2.
T15 1310-1393 Sentence denotes The enzyme is inhibited by fluoride and beta-glycerol phosphate but not by heparin.
T16 1394-1500 Sentence denotes These properties of MAP kinase distinguish it from protein kinases previously described in the literature.
T1 0-77 Sentence denotes Characterization of insulin-stimulated microtubule-associated protein kinase.
T2 78-165 Sentence denotes Rapid isolation and stabilization of a novel serine/threonine kinase from 3T3-L1 cells.
T3 166-445 Sentence denotes A protein kinase, termed microtubule-associated protein (MAP) kinase, which phosphorylates microtubule-associated protein 2 (MAP-2) in vitro and is stimulated 1.5-3-fold in extracts from insulin-treated 3T3-L1 cells has been identified (Ray, L.B., and Sturgill, T.W. (1987) Proc.
T4 446-451 Sentence denotes Natl.
T5 452-457 Sentence denotes Acad.
T6 458-462 Sentence denotes Sci.
T7 463-469 Sentence denotes U.S.A.
T8 470-485 Sentence denotes 84, 1502-1506).
T9 486-619 Sentence denotes Here, we describe chromatographic properties of MAP kinase and provide biochemical characterization of the partially purified enzyme.
T10 620-742 Sentence denotes Isolation of the enzyme is facilitated by its unusually high affinity for hydrophobic interaction chromatography matrices.
T11 743-905 Sentence denotes The molecular weight of the partially purified enzyme was determined to be 35,000 by gel filtration chromatography and 37,000 by glycerol gradient centrifugation.
T12 906-1091 Sentence denotes MAP kinase activity of chromatographic fractions correlated precisely with the presence of a 40-kDa phosphoprotein detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
T13 1092-1157 Sentence denotes MAP kinase has a Km of 7 microM for ATP and does not utilize GTP.
T14 1158-1309 Sentence denotes Acetyl-CoA carboxylase, ATP citrate-lyase, casein, histones, phosvitin, protamine, and ribosomal protein S6 were all poor substrates relative to MAP-2.
T15 1310-1393 Sentence denotes The enzyme is inhibited by fluoride and beta-glycerol phosphate but not by heparin.
T16 1394-1500 Sentence denotes These properties of MAP kinase distinguish it from protein kinases previously described in the literature.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 1385-1392 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T2 1385-1392 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 39-50 Body_part denotes microtubule http://purl.obolibrary.org/obo/GO_0005874
T2 191-202 Body_part denotes microtubule http://purl.obolibrary.org/obo/GO_0005874
T3 257-268 Body_part denotes microtubule http://purl.obolibrary.org/obo/GO_0005874