
PubMed:28326091
Annnotations
pqqtest_sentence
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
M_0 | 1293-1315 | cand:WTO:0000224-flour mechanical property | denotes | nutritional properties |
M_1 | 1293-1315 | cand:funRiceGene:220-nutritional quality | denotes | nutritional properties |
M_2 | 1293-1315 | cand:TO:0000489-carbohydrate composition related trait | denotes | nutritional properties |
M_3 | 1293-1315 | cand:WTO:0000223-flour composition | denotes | nutritional properties |
M_4 | 1293-1315 | cand:WTO:0000162-micronutrient use efficiency | denotes | nutritional properties |
M_5 | 1293-1315 | cand:WTO:0000159-macronutrient use efficiency | denotes | nutritional properties |
M_6 | 1293-1315 | cand:funRiceGene:481-actin protein | denotes | nutritional properties |
M_7 | 1293-1315 | cand:PO:0008007-nucellar projection | denotes | nutritional properties |
M_8 | 1293-1315 | cand:TO:0000890-seed coat proportion | denotes | nutritional properties |
M_9 | 1293-1315 | cand:WTO:0000109-soil property | denotes | nutritional properties |
M_10 | 1100-1117 | cand:TO:0000613-groat percentage | denotes | higher proportion |
M_11 | 1100-1117 | cand:PPO:0000003-lower percent | denotes | higher proportion |
M_12 | 1100-1117 | cand:TO:0000570-watery lesion percentage | denotes | higher proportion |
M_13 | 1100-1117 | cand:WTO:0000173-percentage of florets without grain | denotes | higher proportion |
M_14 | 1100-1117 | cand:TO:0000725-megasporocyte number | denotes | higher proportion |
M_15 | 1100-1117 | cand:PPO:0000001-lower count | denotes | higher proportion |
M_16 | 1100-1117 | cand:PO:0004706-flower primordium | denotes | higher proportion |
M_17 | 1100-1117 | cand:TO:0000890-seed coat proportion | denotes | higher proportion |
M_18 | 1100-1117 | cand:funRiceGene:210-HIGHER YIELD RICE | denotes | higher proportion |
M_19 | 1100-1117 | cand:PO:0006089-anther primordium | denotes | higher proportion |
M_20 | 1100-1117 | cand:WTO:0000049-reproduction | denotes | higher proportion |
M_21 | 1413-1430 | cand:TO:0000372-amylose to amylopectin ratio | denotes | high-amylose rice |
M_22 | 1413-1430 | cand:TO:0002655-starch grain size | denotes | high-amylose rice |
M_23 | 1413-1430 | cand:TO:0000098-glutinous endosperm | denotes | high-amylose rice |
M_24 | 1413-1430 | cand:TO:0000144-milled rice yield | denotes | high-amylose rice |
M_25 | 1413-1430 | cand:WTO:0000149-grain starch content | denotes | high-amylose rice |
M_26 | 1413-1430 | cand:TO:0002658-starch grain synthesis | denotes | high-amylose rice |
M_27 | 1413-1430 | cand:funRiceGene:100-heat tolerance | denotes | high-amylose rice |
M_28 | 1413-1430 | cand:PECO:0007196-light exposure | denotes | high-amylose rice |
M_29 | 1413-1430 | cand:PO:0030018-gametophore | denotes | high-amylose rice |
M_30 | 1413-1430 | cand:PO:0025141-phyllome tip | denotes | high-amylose rice |
M_31 | 1413-1430 | cand:funRiceGene:210-HIGHER YIELD RICE | denotes | high-amylose rice |
M_32 | 1194-1211 | cand:TO:0000696-starch content | denotes | AC and RS content |
M_33 | 1194-1211 | cand:TO:0000993-cellulose content | denotes | AC and RS content |
M_34 | 1194-1211 | cand:WTO:0000164-moisture content of the grain | denotes | AC and RS content |
M_35 | 1194-1211 | cand:TO:1000063-shoot system magnesium content | denotes | AC and RS content |
M_36 | 1194-1211 | cand:WTO:0000025-high grain protein content | denotes | AC and RS content |
M_37 | 1194-1211 | cand:TO:0000602-total fat content | denotes | AC and RS content |
M_38 | 1194-1211 | cand:TO:0020091-manganese content | denotes | AC and RS content |
M_39 | 1194-1211 | cand:TO:0000955-cyanide content | denotes | AC and RS content |
M_40 | 1194-1211 | cand:TO:0000932-mannose content | denotes | AC and RS content |
M_41 | 1194-1211 | cand:TO:0000466-carbon content | denotes | AC and RS content |
M_42 | 1194-1211 | cand:TO:0000333-sugar content | denotes | AC and RS content |
M_43 | 1079-1084 | GeneID:NA | denotes | sbeII |
M_44 | 1471-1477 | GeneID:RAPID:Os02g0528200MSUID:LOC_Os02g32660GraID:GR:0060839 | denotes | SBEIIb |
M_45 | 1051-1055 | GeneID:NA | denotes | sbeI |
M_46 | 1079-1083 | GeneID:NA | denotes | sbeI |
T91389 | 152-161 | funRiceGene:269 | denotes | resistant |
T35372 | 1293-1315 | xzyao:44798 | denotes | nutritional properties |
T79842 | 85-109 | xzyao:17013 | denotes | Starch Branching Enzymes |
T84812 | 301-317 | xzyao:2611 | denotes | branching enzyme |
T16205 | 1100-1117 | xzyao:23774 | denotes | higher proportion |
T9369 | 230-250 | xzyao:30463 | denotes | chemical mutagenesis |
T80324 | 1261-1288 | xzyao:28204 | denotes | thus altered fine structure |
T369 | 127-142 | TO:0000196, xzyao:37574 | denotes | amylose content |
T67159 | 230-238 | WTO:0000069 | denotes | chemical |
T76988 | 381-400 | xzyao:623 | denotes | physical properties |
T85531 | 254-270 | xzyao:33663 | denotes | RNA interference |
T99366 | 85-91 | funRiceGene:143 | denotes | Starch |
T25488 | 162-168 | funRiceGene:143 | denotes | starch |
T59984 | 294-300 | funRiceGene:143 | denotes | starch |
T96252 | 404-410 | funRiceGene:143 | denotes | starch |
T25564 | 455-461 | funRiceGene:143 | denotes | starch |
T37865 | 1319-1325 | funRiceGene:143 | denotes | starch |
T94219 | 92-101 | funRiceGene:525 | denotes | Branching |
T24267 | 301-310 | funRiceGene:525 | denotes | branching |
T10399 | 462-471 | funRiceGene:525 | denotes | branching |
T71543 | 1201-1211 | xzyao:19829 | denotes | RS content |
T39447 | 294-317 | xzyao:3088 | denotes | starch branching enzyme |
T41144 | 111-118 | hunflair:NA:Species | denotes | Cereals |
T82624 | 565-569 | hunflair:NA:Gene | denotes | SBEI |
T51333 | 671-675 | hunflair:NA:Gene | denotes | SBEI |
T98959 | 40-46 | hunflair:NA:Gene | denotes | CRISPR |
T62308 | 506-512 | hunflair:NA:Gene | denotes | CRISPR |
T35967 | 1439-1445 | hunflair:NA:Gene | denotes | CRISPR |
T45001 | 744-763 | hunflair:NA:CellLine | denotes | homozygous T0 lines |
T99001 | 1079-1084 | hunflair:NA:Gene | denotes | sbeII |
T29306 | 319-322 | hunflair:NA:Gene | denotes | SBE |
T2418 | 1051-1055 | hunflair:NA:Gene | denotes | sbeI |
T55736 | 47-60 | hunflair:NA:Gene | denotes | Cas9-Mediated |
T57229 | 27-31 | hunflair:NA:Species | denotes | Rice |
T46822 | 574-580 | hunflair:NA:Gene | denotes | SBEIIb |
T61656 | 680-686 | hunflair:NA:Gene | denotes | SBEIIb |
T73684 | 1471-1477 | hunflair:NA:Gene | denotes | SBEIIb |
T53438 | 1446-1459 | hunflair:NA:Gene | denotes | Cas9-mediated |
T50990 | 821-837 | hunflair:NA:CellLine | denotes | bi-allelic lines |
T26689 | 584-588 | hunflair:NA:Species | denotes | rice |
T1120 | 1426-1430 | hunflair:NA:Species | denotes | rice |
T14751 | 294-317 | hunflair:NA:Gene | denotes | starch branching enzyme |
T29034 | 584-588 | pubtator:4530:Species | denotes | rice |
T7013 | 1426-1430 | pubtator:4530:Species | denotes | rice |
T39075 | 27-31 | pubtator:4530:Species | denotes | Rice |
T1547 | 85-101 | pubtator:MESH:D015356:Disease | denotes | Starch Branching |
21k_plant_trait_mention
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
M_0 | 230-238 | WTO:0000069 | denotes | chemical |
M_1 | 162-168 | funRiceGene:143 | denotes | starch |
M_2 | 294-300 | funRiceGene:143 | denotes | starch |
M_3 | 455-461 | funRiceGene:143 | denotes | starch |
M_4 | 127-142 | xzyao:2145 | denotes | amylose content |
M_5 | 301-310 | funRiceGene:525 | denotes | branching |
M_6 | 462-471 | funRiceGene:525 | denotes | branching |
M_7 | 152-161 | funRiceGene:269 | denotes | resistant |
M_8 | 127-142 | TO:0000196, xzyao:11214 | denotes | amylose content |
M_9 | 319-322 | hunflair:NA:Gene | denotes | SBE |
M_10 | 1051-1055 | hunflair:NA:Gene | denotes | sbeI |
M_11 | 294-317 | hunflair:NA:Gene | denotes | starch branching enzyme |
M_12 | 821-837 | hunflair:NA:CellLine | denotes | bi-allelic lines |
M_13 | 744-763 | hunflair:NA:CellLine | denotes | homozygous T0 lines |
M_14 | 47-60 | hunflair:NA:Gene | denotes | Cas9-Mediated |
M_15 | 584-588 | hunflair:NA:Species | denotes | rice |
M_16 | 1426-1430 | hunflair:NA:Species | denotes | rice |
M_17 | 1079-1084 | hunflair:NA:Gene | denotes | sbeII |
M_18 | 111-118 | hunflair:NA:Species | denotes | Cereals |
M_19 | 40-46 | hunflair:NA:Gene | denotes | CRISPR |
M_20 | 506-512 | hunflair:NA:Gene | denotes | CRISPR |
M_21 | 1439-1445 | hunflair:NA:Gene | denotes | CRISPR |
M_22 | 27-31 | hunflair:NA:Species | denotes | Rice |
M_23 | 1446-1459 | hunflair:NA:Gene | denotes | Cas9-mediated |
M_24 | 565-569 | hunflair:NA:Gene | denotes | SBEI |
M_25 | 671-675 | hunflair:NA:Gene | denotes | SBEI |
M_26 | 574-580 | hunflair:NA:Gene | denotes | SBEIIb |
M_27 | 680-686 | hunflair:NA:Gene | denotes | SBEIIb |
M_28 | 1471-1477 | hunflair:NA:Gene | denotes | SBEIIb |
M_29 | 85-101 | pubtator:MESH:D015356:Disease | denotes | Starch Branching |
M_30 | 27-31 | pubtator:4530:Species | denotes | Rice |
M_31 | 584-588 | pubtator:4530:Species | denotes | rice |
M_32 | 1426-1430 | pubtator:4530:Species | denotes | rice |
OryzaGP_2022
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 14-26 | http://identifiers.org/oryzabase.gene/25 | denotes | High-Amylose |
T2 | 1413-1425 | http://identifiers.org/oryzabase.gene/25 | denotes | high-amylose |
OryzaGP_2021_v2
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 565-569 | http://identifiers.org/oryzabase.gene/730 | denotes | SBEI |
T2 | 574-580 | http://identifiers.org/oryzabase.gene/503 | denotes | SBEIIb |
T3 | 671-675 | http://identifiers.org/oryzabase.gene/730 | denotes | SBEI |
T4 | 680-686 | http://identifiers.org/oryzabase.gene/503 | denotes | SBEIIb |
T5 | 1471-1477 | http://identifiers.org/oryzabase.gene/503 | denotes | SBEIIb |
T23264 | 565-569 | http://identifiers.org/rapdb.locus/Os06g0726400 | denotes | SBEI |
T5986 | 574-580 | http://identifiers.org/rapdb.locus/Os02g0528200 | denotes | SBEIIb |
T3640 | 671-675 | http://identifiers.org/rapdb.locus/Os06g0726400 | denotes | SBEI |
T11240 | 680-686 | http://identifiers.org/rapdb.locus/Os02g0528200 | denotes | SBEIIb |
T77547 | 1471-1477 | http://identifiers.org/rapdb.locus/Os02g0528200 | denotes | SBEIIb |
OryzaGP_2021_FLAIR
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
M_0 | 744-763 | hunflair:NA:CellLine | denotes | homozygous T0 lines |
M_1 | 1079-1084 | hunflair:NA:Gene | denotes | sbeII |
M_2 | 27-31 | hunflair:NA:Species | denotes | Rice |
M_3 | 821-837 | hunflair:NA:CellLine | denotes | bi-allelic lines |
M_4 | 47-60 | hunflair:NA:Gene | denotes | Cas9-Mediated |
M_5 | 1051-1055 | hunflair:NA:Gene | denotes | sbeI |
M_6 | 565-569 | hunflair:NA:Gene | denotes | SBEI |
M_7 | 671-675 | hunflair:NA:Gene | denotes | SBEI |
M_8 | 584-588 | hunflair:NA:Species | denotes | rice |
M_9 | 1426-1430 | hunflair:NA:Species | denotes | rice |
M_10 | 319-322 | hunflair:NA:Gene | denotes | SBE |
M_11 | 40-46 | hunflair:NA:Gene | denotes | CRISPR |
M_12 | 506-512 | hunflair:NA:Gene | denotes | CRISPR |
M_13 | 1439-1445 | hunflair:NA:Gene | denotes | CRISPR |
M_14 | 294-317 | hunflair:NA:Gene | denotes | starch branching enzyme |
M_15 | 111-118 | hunflair:NA:Species | denotes | Cereals |
M_16 | 1446-1459 | hunflair:NA:Gene | denotes | Cas9-mediated |
M_17 | 574-580 | hunflair:NA:Gene | denotes | SBEIIb |
M_18 | 680-686 | hunflair:NA:Gene | denotes | SBEIIb |
M_19 | 1471-1477 | hunflair:NA:Gene | denotes | SBEIIb |
OryzaGP_2021
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 574-580 | http://identifiers.org/oryzabase.gene/503 | denotes | SBEIIb |
T2 | 680-686 | http://identifiers.org/oryzabase.gene/503 | denotes | SBEIIb |
T3 | 1471-1477 | http://identifiers.org/oryzabase.gene/503 | denotes | SBEIIb |
T95648 | 565-569 | http://identifiers.org/ricegap/LOC_Os06g51084 | denotes | SBEI |
T48868 | 574-580 | http://identifiers.org/ricegap/LOC_Os02g32660 | denotes | SBEIIb |
T6102 | 671-675 | http://identifiers.org/ricegap/LOC_Os06g51084 | denotes | SBEI |
T4 | 680-686 | http://identifiers.org/ricegap/LOC_Os02g32660 | denotes | SBEIIb |
T5 | 1051-1055 | http://identifiers.org/ricegap/LOC_Os06g51084 | denotes | sbeI |
T6 | 1471-1477 | http://identifiers.org/ricegap/LOC_Os02g32660 | denotes | SBEIIb |
T356 | 565-569 | http://identifiers.org/rapdb.locus/Os06g0726400 | denotes | SBEI |
T57291 | 574-580 | http://identifiers.org/rapdb.locus/Os02g0528200 | denotes | SBEIIb |
T38792 | 671-675 | http://identifiers.org/rapdb.locus/Os06g0726400 | denotes | SBEI |
T21898 | 680-686 | http://identifiers.org/rapdb.locus/Os02g0528200 | denotes | SBEIIb |
T84220 | 1051-1055 | http://identifiers.org/rapdb.locus/Os06g0726400 | denotes | sbeI |
T12050 | 1471-1477 | http://identifiers.org/rapdb.locus/Os02g0528200 | denotes | SBEIIb |
T55540 | 574-580 | http://identifiers.org/uniprot/Q6H6P8 | denotes | SBEIIb |
T39790 | 680-686 | http://identifiers.org/uniprot/Q6H6P8 | denotes | SBEIIb |
T47025 | 1471-1477 | http://identifiers.org/uniprot/Q6H6P8 | denotes | SBEIIb |
M_0 | 744-763 | hunflair:NA:CellLine | denotes | homozygous T0 lines |
M_1 | 1079-1084 | hunflair:NA:Gene | denotes | sbeII |
M_2 | 27-31 | hunflair:NA:Species | denotes | Rice |
M_3 | 821-837 | hunflair:NA:CellLine | denotes | bi-allelic lines |
M_4 | 47-60 | hunflair:NA:Gene | denotes | Cas9-Mediated |
M_5 | 1051-1055 | hunflair:NA:Gene | denotes | sbeI |
M_6 | 565-569 | hunflair:NA:Gene | denotes | SBEI |
M_7 | 671-675 | hunflair:NA:Gene | denotes | SBEI |
M_8 | 584-588 | hunflair:NA:Species | denotes | rice |
M_9 | 1426-1430 | hunflair:NA:Species | denotes | rice |
M_10 | 319-322 | hunflair:NA:Gene | denotes | SBE |
M_11 | 40-46 | hunflair:NA:Gene | denotes | CRISPR |
M_12 | 506-512 | hunflair:NA:Gene | denotes | CRISPR |
M_13 | 1439-1445 | hunflair:NA:Gene | denotes | CRISPR |
M_14 | 294-317 | hunflair:NA:Gene | denotes | starch branching enzyme |
M_15 | 111-118 | hunflair:NA:Species | denotes | Cereals |
M_16 | 1446-1459 | hunflair:NA:Gene | denotes | Cas9-mediated |
M_17 | 574-580 | hunflair:NA:Gene | denotes | SBEIIb |
M_18 | 680-686 | hunflair:NA:Gene | denotes | SBEIIb |
M_19 | 1471-1477 | hunflair:NA:Gene | denotes | SBEIIb |
funRiceGenes-all
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
PTO-all_T1 | 85-91 | http://purl.obolibrary.org/obo/TO_0000698 | denotes | Starch |
PTO-all_T2 | 127-142 | http://purl.obolibrary.org/obo/TO_0000196 | denotes | amylose content |
PTO-all_T3 | 144-146 | http://purl.obolibrary.org/obo/TO_0000294 | denotes | AC |
PTO-all_T4 | 162-168 | http://purl.obolibrary.org/obo/TO_0000698 | denotes | starch |
PTO-all_T5 | 294-300 | http://purl.obolibrary.org/obo/TO_0000698 | denotes | starch |
PTO-all_T6 | 404-410 | http://purl.obolibrary.org/obo/TO_0000698 | denotes | starch |
PTO-all_T7 | 455-461 | http://purl.obolibrary.org/obo/TO_0000698 | denotes | starch |
PTO-all_T8 | 1194-1196 | http://purl.obolibrary.org/obo/TO_0000294 | denotes | AC |
PTO-all_T9 | 1319-1325 | http://purl.obolibrary.org/obo/TO_0000698 | denotes | starch |
funRiceGenes-exact
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
PTO-exact_T1 | 127-142 | http://purl.obolibrary.org/obo/TO_0000196 | denotes | amylose content |
Oryza_sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 0-110 | Sentence | denotes | Generation of High-Amylose Rice through CRISPR/Cas9-Mediated Targeted Mutagenesis of Starch Branching Enzymes. |
T2 | 111-206 | Sentence | denotes | Cereals high in amylose content (AC) and resistant starch (RS) offer potential health benefits. |
T3 | 207-411 | Sentence | denotes | Previous studies using chemical mutagenesis or RNA interference have demonstrated that starch branching enzyme (SBE) plays a major role in determining the fine structure and physical properties of starch. |
T4 | 412-492 | Sentence | denotes | However, it remains a challenge to control starch branching in commercial lines. |
T5 | 493-589 | Sentence | denotes | Here, we use CRISPR/Cas9 technology to generate targeted mutagenesis in SBEI and SBEIIb in rice. |
T6 | 590-726 | Sentence | denotes | The frequencies of obtained homozygous or bi-allelic mutant lines with indels in SBEI and SBEIIb in T0 generation were from 26.7 to 40%. |
T7 | 727-872 | Sentence | denotes | Mutations in the homozygous T0 lines stably transmitted to the T1 generation and those in the bi-allelic lines segregated in a Mendelian fashion. |
T8 | 873-993 | Sentence | denotes | Transgene-free plants carrying only the frame-shifted mutagenesis were recovered in T1 generation following segregation. |
T9 | 994-1326 | Sentence | denotes | Whereas no obvious differences were observed between the sbeI mutants and wild type, sbeII mutants showed higher proportion of long chains presented in debranched amylopectin, significantly increased AC and RS content to as higher as 25.0 and 9.8%, respectively, and thus altered fine structure and nutritional properties of starch. |
T10 | 1327-1478 | Sentence | denotes | Taken together, our results demonstrated for the first time the feasibility to create high-amylose rice through CRISPR/Cas9-mediated editing of SBEIIb. |
Oryza_sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 0-110 | Sentence | denotes | Generation of High-Amylose Rice through CRISPR/Cas9-Mediated Targeted Mutagenesis of Starch Branching Enzymes. |
T2 | 111-206 | Sentence | denotes | Cereals high in amylose content (AC) and resistant starch (RS) offer potential health benefits. |
T3 | 207-411 | Sentence | denotes | Previous studies using chemical mutagenesis or RNA interference have demonstrated that starch branching enzyme (SBE) plays a major role in determining the fine structure and physical properties of starch. |
T4 | 412-492 | Sentence | denotes | However, it remains a challenge to control starch branching in commercial lines. |
T5 | 493-589 | Sentence | denotes | Here, we use CRISPR/Cas9 technology to generate targeted mutagenesis in SBEI and SBEIIb in rice. |
T6 | 590-726 | Sentence | denotes | The frequencies of obtained homozygous or bi-allelic mutant lines with indels in SBEI and SBEIIb in T0 generation were from 26.7 to 40%. |
T7 | 727-872 | Sentence | denotes | Mutations in the homozygous T0 lines stably transmitted to the T1 generation and those in the bi-allelic lines segregated in a Mendelian fashion. |
T8 | 873-993 | Sentence | denotes | Transgene-free plants carrying only the frame-shifted mutagenesis were recovered in T1 generation following segregation. |
T9 | 994-1326 | Sentence | denotes | Whereas no obvious differences were observed between the sbeI mutants and wild type, sbeII mutants showed higher proportion of long chains presented in debranched amylopectin, significantly increased AC and RS content to as higher as 25.0 and 9.8%, respectively, and thus altered fine structure and nutritional properties of starch. |
T10 | 1327-1478 | Sentence | denotes | Taken together, our results demonstrated for the first time the feasibility to create high-amylose rice through CRISPR/Cas9-mediated editing of SBEIIb. |
Oryza_sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 0-110 | Sentence | denotes | Generation of High-Amylose Rice through CRISPR/Cas9-Mediated Targeted Mutagenesis of Starch Branching Enzymes. |
T2 | 111-206 | Sentence | denotes | Cereals high in amylose content (AC) and resistant starch (RS) offer potential health benefits. |
T3 | 207-411 | Sentence | denotes | Previous studies using chemical mutagenesis or RNA interference have demonstrated that starch branching enzyme (SBE) plays a major role in determining the fine structure and physical properties of starch. |
T4 | 412-492 | Sentence | denotes | However, it remains a challenge to control starch branching in commercial lines. |
T5 | 493-589 | Sentence | denotes | Here, we use CRISPR/Cas9 technology to generate targeted mutagenesis in SBEI and SBEIIb in rice. |
T6 | 590-726 | Sentence | denotes | The frequencies of obtained homozygous or bi-allelic mutant lines with indels in SBEI and SBEIIb in T0 generation were from 26.7 to 40%. |
T7 | 727-872 | Sentence | denotes | Mutations in the homozygous T0 lines stably transmitted to the T1 generation and those in the bi-allelic lines segregated in a Mendelian fashion. |
T8 | 873-993 | Sentence | denotes | Transgene-free plants carrying only the frame-shifted mutagenesis were recovered in T1 generation following segregation. |
T9 | 994-1326 | Sentence | denotes | Whereas no obvious differences were observed between the sbeI mutants and wild type, sbeII mutants showed higher proportion of long chains presented in debranched amylopectin, significantly increased AC and RS content to as higher as 25.0 and 9.8%, respectively, and thus altered fine structure and nutritional properties of starch. |
T10 | 1327-1478 | Sentence | denotes | Taken together, our results demonstrated for the first time the feasibility to create high-amylose rice through CRISPR/Cas9-mediated editing of SBEIIb. |
Oryza_sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 0-110 | Sentence | denotes | Generation of High-Amylose Rice through CRISPR/Cas9-Mediated Targeted Mutagenesis of Starch Branching Enzymes. |
T2 | 111-206 | Sentence | denotes | Cereals high in amylose content (AC) and resistant starch (RS) offer potential health benefits. |
T3 | 207-411 | Sentence | denotes | Previous studies using chemical mutagenesis or RNA interference have demonstrated that starch branching enzyme (SBE) plays a major role in determining the fine structure and physical properties of starch. |
T4 | 412-492 | Sentence | denotes | However, it remains a challenge to control starch branching in commercial lines. |
T5 | 493-589 | Sentence | denotes | Here, we use CRISPR/Cas9 technology to generate targeted mutagenesis in SBEI and SBEIIb in rice. |
T6 | 590-726 | Sentence | denotes | The frequencies of obtained homozygous or bi-allelic mutant lines with indels in SBEI and SBEIIb in T0 generation were from 26.7 to 40%. |
T7 | 727-872 | Sentence | denotes | Mutations in the homozygous T0 lines stably transmitted to the T1 generation and those in the bi-allelic lines segregated in a Mendelian fashion. |
T8 | 873-993 | Sentence | denotes | Transgene-free plants carrying only the frame-shifted mutagenesis were recovered in T1 generation following segregation. |
T9 | 994-1326 | Sentence | denotes | Whereas no obvious differences were observed between the sbeI mutants and wild type, sbeII mutants showed higher proportion of long chains presented in debranched amylopectin, significantly increased AC and RS content to as higher as 25.0 and 9.8%, respectively, and thus altered fine structure and nutritional properties of starch. |
T10 | 1327-1478 | Sentence | denotes | Taken together, our results demonstrated for the first time the feasibility to create high-amylose rice through CRISPR/Cas9-mediated editing of SBEIIb. |