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sentences

Id Subject Object Predicate Lexical cue
T1 0-89 Sentence denotes Biosynthesis in Escherichia coli of sn-glycerol 3-phosphate, a precursor of phospholipid.
T2 90-215 Sentence denotes Kinetic characterization of wild type and feedback-resistant forms of the biosynthetic sn-glycerol-3-phosphate dehydrogenase.
T3 216-419 Sentence denotes Homogeneous wild type and feedback-resistant forms of the biosynthetic sn-glycerol 3-phosphate (glycerol-P) dehydrogenase of Escherichia coli (EC1.1.1.8) were subjected to two-substrate kinetic analysis.
T4 420-631 Sentence denotes The kinetics of the NADPH-dependent reduction of dihydroxyacetone phosphate (dihydroxyacetone-P) and of the NADP-dependent oxidation of glycerol-P indicate that these reactions proceed by a sequential mechanism.
T5 632-723 Sentence denotes Glycerol-P was a competitive inhibitor with respect to dihydroxyacetone-P for both enzymes.
T6 724-866 Sentence denotes The wild type and feedback-resistant glycerol-P dehydrogenases had Ki values for glycerol-P of 4.4 micrometer and 43 micrometer, respectively.
T7 867-1061 Sentence denotes Therefore, the sensitivity of the wild type activity and reduced sensitivity of the feedback-resistant activity, both noted previously in crude extracts, were inherent properties of the enzymes.
T8 1062-1273 Sentence denotes The patterns of product inhibition for both enzymes were identical, and the difference in the inhibition constants for glycerol-P occurred without significant alteration of any other kinetic constant determined.
T9 1274-1407 Sentence denotes Kinetic mechanisms consistent with the patterns of product inhibition violated Haldane relationships and other kinetic relationships.
T10 1408-1510 Sentence denotes These discrepancies suggest that glycerol-P inhibition occurs at a site distinct from the active site.
T11 1511-1661 Sentence denotes The pH dependencies of the Km for dihydroxyacetone-P and the Ki for glycerol-P were markedly different suggesting the existence of an allosteric site.
T12 1662-1767 Sentence denotes The addition of glycerol-P in the presence of NADPH stabilized both enzymes against thermal inactivation.
T13 1768-1916 Sentence denotes Half-maximal stabilization was provided by 5 micrometer and 50 micrometer glycerol-P for the wild type and feedback-resistant enzymes, respectively.
T14 1917-2127 Sentence denotes These kinetic data, considered in conjunction with previous physiologic and genetic data, indicate that the synthesis of glycerol-P is regulated in vivo by glycerol-P inhibition of the glycerol-P dehydrogenase.
T15 2128-2213 Sentence denotes The data suggest that glycerol-P inhibition occurs at an allosteric, regulatory site.
T1 0-89 Sentence denotes Biosynthesis in Escherichia coli of sn-glycerol 3-phosphate, a precursor of phospholipid.
T2 90-215 Sentence denotes Kinetic characterization of wild type and feedback-resistant forms of the biosynthetic sn-glycerol-3-phosphate dehydrogenase.
T3 216-419 Sentence denotes Homogeneous wild type and feedback-resistant forms of the biosynthetic sn-glycerol 3-phosphate (glycerol-P) dehydrogenase of Escherichia coli (EC1.1.1.8) were subjected to two-substrate kinetic analysis.
T4 420-631 Sentence denotes The kinetics of the NADPH-dependent reduction of dihydroxyacetone phosphate (dihydroxyacetone-P) and of the NADP-dependent oxidation of glycerol-P indicate that these reactions proceed by a sequential mechanism.
T5 632-723 Sentence denotes Glycerol-P was a competitive inhibitor with respect to dihydroxyacetone-P for both enzymes.
T6 724-866 Sentence denotes The wild type and feedback-resistant glycerol-P dehydrogenases had Ki values for glycerol-P of 4.4 micrometer and 43 micrometer, respectively.
T7 867-1061 Sentence denotes Therefore, the sensitivity of the wild type activity and reduced sensitivity of the feedback-resistant activity, both noted previously in crude extracts, were inherent properties of the enzymes.
T8 1062-1273 Sentence denotes The patterns of product inhibition for both enzymes were identical, and the difference in the inhibition constants for glycerol-P occurred without significant alteration of any other kinetic constant determined.
T9 1274-1407 Sentence denotes Kinetic mechanisms consistent with the patterns of product inhibition violated Haldane relationships and other kinetic relationships.
T10 1408-1510 Sentence denotes These discrepancies suggest that glycerol-P inhibition occurs at a site distinct from the active site.
T11 1511-1661 Sentence denotes The pH dependencies of the Km for dihydroxyacetone-P and the Ki for glycerol-P were markedly different suggesting the existence of an allosteric site.
T12 1662-1767 Sentence denotes The addition of glycerol-P in the presence of NADPH stabilized both enzymes against thermal inactivation.
T13 1768-1916 Sentence denotes Half-maximal stabilization was provided by 5 micrometer and 50 micrometer glycerol-P for the wild type and feedback-resistant enzymes, respectively.
T14 1917-2127 Sentence denotes These kinetic data, considered in conjunction with previous physiologic and genetic data, indicate that the synthesis of glycerol-P is regulated in vivo by glycerol-P inhibition of the glycerol-P dehydrogenase.
T15 2128-2213 Sentence denotes The data suggest that glycerol-P inhibition occurs at an allosteric, regulatory site.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 16-32 OrganismTaxon denotes Escherichia coli 562
T2 341-357 OrganismTaxon denotes Escherichia coli 562