PubMed:28293289 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/28293289","sourcedb":"PubMed","sourceid":"28293289","text":"Novel Acinetobacter parvus HANDI 309 microbial biomass for the production of N-acetyl-β-d-glucosamine (GlcNAc) using swollen chitin substrate in submerged fermentation.\nBACKGROUND: N-acetyl-β-d-glucosamine (GlcNAc)6 is extensively used as an important bio-agent and a functional food additive. The traditional chemical process for GlcNAc production has some problems such as high production cost, low yield, and acidic pollution. Therefore, to discover a novel chitinase that is suitable for bioconversion of chitin to GlcNAc would be of great value.\nRESULTS: Here, we describe the complete isolation and functional characterization of a novel exo-chitinase from Acinetobacter parvus HANDI 309 for the conversion of chitin. The identified exo-chitinase mainly produced N-acetyl-d-glucosamine, using chitin as a substrate by submerged fermentation. The A. parvus HANDI 309 biofuels producing exo-chitinase were characterized by TLC, and was further validated and quantified by HPLC. Furthermore, the optimal temperature and pH for the exo-chitinase activity was obtained in the culture conditions of 30 °C and 7.0, respectively. The maximum growth of the stationary phase was reached in 24 h after incubation. These results suggest that A. parvus HANDI 309 biofuels producing exo-chitinases may have great potential in chitin to N-acetyl-d-glucosamine conversion.\nCONCLUSIONS: The excellent thermostability and hydrolytic properties may give the exo-chitinase great potential in chitin to GlcNAc conversion in industry. This is the first report that A. parvus HANDI 309 is a novel bacterial strain that has the ability to produce an enormous amount of exo-chitinase-producing bio-agents in a short time on an industrial scale without any pretreatment, as well as being potentially valuable in the food and pharmaceutical industries.","tracks":[{"project":"NGLY1-deficiency","denotations":[{"id":"PD-NGLY1-deficiency-B_T1","span":{"begin":77,"end":101},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T2","span":{"begin":103,"end":109},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T3","span":{"begin":181,"end":205},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T4","span":{"begin":207,"end":213},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T5","span":{"begin":331,"end":337},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T6","span":{"begin":519,"end":525},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T7","span":{"begin":1488,"end":1494},"obj":"chem:24139"}],"namespaces":[{"prefix":"hgnc","uri":"https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:"},{"prefix":"omim","uri":"https://www.omim.org/entry/"},{"prefix":"chem","uri":"https://pubchem.ncbi.nlm.nih.gov/compound/"}],"attributes":[{"subj":"PD-NGLY1-deficiency-B_T1","pred":"source","obj":"NGLY1-deficiency"},{"subj":"PD-NGLY1-deficiency-B_T2","pred":"source","obj":"NGLY1-deficiency"},{"subj":"PD-NGLY1-deficiency-B_T3","pred":"source","obj":"NGLY1-deficiency"},{"subj":"PD-NGLY1-deficiency-B_T4","pred":"source","obj":"NGLY1-deficiency"},{"subj":"PD-NGLY1-deficiency-B_T5","pred":"source","obj":"NGLY1-deficiency"},{"subj":"PD-NGLY1-deficiency-B_T6","pred":"source","obj":"NGLY1-deficiency"},{"subj":"PD-NGLY1-deficiency-B_T7","pred":"source","obj":"NGLY1-deficiency"}]},{"project":"ngly1-sample2","denotations":[{"id":"T1","span":{"begin":519,"end":525},"obj":"chem:24139"},{"id":"T2","span":{"begin":77,"end":101},"obj":"chem:24139"},{"id":"T3","span":{"begin":103,"end":109},"obj":"chem:24139"},{"id":"T4","span":{"begin":207,"end":213},"obj":"chem:24139"},{"id":"T5","span":{"begin":181,"end":205},"obj":"chem:24139"},{"id":"T6","span":{"begin":331,"end":337},"obj":"chem:24139"},{"id":"T7","span":{"begin":769,"end":791},"obj":"chem:24139"},{"id":"T8","span":{"begin":1328,"end":1350},"obj":"chem:24139"},{"id":"T9","span":{"begin":1488,"end":1494},"obj":"chem:24139"}],"attributes":[{"subj":"T1","pred":"source","obj":"ngly1-sample2"},{"subj":"T2","pred":"source","obj":"ngly1-sample2"},{"subj":"T3","pred":"source","obj":"ngly1-sample2"},{"subj":"T4","pred":"source","obj":"ngly1-sample2"},{"subj":"T5","pred":"source","obj":"ngly1-sample2"},{"subj":"T6","pred":"source","obj":"ngly1-sample2"},{"subj":"T7","pred":"source","obj":"ngly1-sample2"},{"subj":"T8","pred":"source","obj":"ngly1-sample2"},{"subj":"T9","pred":"source","obj":"ngly1-sample2"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"NGLY1-deficiency","color":"#c493ec","default":true},{"id":"ngly1-sample2","color":"#93ecaa"}]}]}}