| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-165 |
Sentence |
denotes |
Signal transduction via the beta 1 integrins is a required intermediate in interleukin-1 beta induction of alkaline phosphatase activity in human osteosarcoma cells. |
| TextSentencer_T2 |
166-408 |
Sentence |
denotes |
In this report data are presented which demonstrate that the induction of an osteoblastic phenotype by interleukin-1 beta (IL-1 beta) requires an intermediate step involving signal transduction via the beta 1 family of integrin glycoproteins. |
| TextSentencer_T3 |
409-614 |
Sentence |
denotes |
Recombinant human IL-1 beta inhibits human osteosarcoma cell proliferation, stimulates integrin expression, and induces alkaline phosphatase activity, a marker of osteoinductive and osteoblastic phenotype. |
| TextSentencer_T4 |
615-838 |
Sentence |
denotes |
The approximately 10-fold stimulation of expression of the beta 1 integrins occurs rapidly (within 20 to 40 h), whereas the alkaline phosphatase activity is not induced until at least 5 days after the addition of IL-1 beta. |
| TextSentencer_T5 |
839-1136 |
Sentence |
denotes |
To determine whether the early stimulation of integrin expression is required for the subsequent expression of alkaline phosphatase activity, polyclonal as well as monoclonal antibodies directed against the alpha 5 and beta 1 integrin subunits were added to cultures at the same time as IL-1 beta. |
| TextSentencer_T6 |
1137-1402 |
Sentence |
denotes |
These antibodies inhibited by 55 to 82% the longer term induction of the osteoblastic differentiation marker, alkaline phosphatase activity, but did not however affect the IL-1 beta-induced stimulation of integrin expression or the inhibition of cell proliferation. |
| TextSentencer_T7 |
1403-1501 |
Sentence |
denotes |
In addition, at the concentrations used, there was no effect of the antibodies on cell attachment. |
| TextSentencer_T8 |
1502-1748 |
Sentence |
denotes |
These data suggest that the stimulation of integrin expression by IL-1 beta, and the resulting enhanced integrin-extracellular matrix interactions, is a required intermediate event in the IL-1 beta regulation of osteoblastic cell differentiation. |
| TextSentencer_T9 |
1749-1960 |
Sentence |
denotes |
The data also suggest that the integrins are capable of signal transduction resulting in altered gene expression, and may also play a crucial role in modulating cytokine-mediated effects on cell differentiation. |
| T1 |
0-165 |
Sentence |
denotes |
Signal transduction via the beta 1 integrins is a required intermediate in interleukin-1 beta induction of alkaline phosphatase activity in human osteosarcoma cells. |
| T2 |
166-408 |
Sentence |
denotes |
In this report data are presented which demonstrate that the induction of an osteoblastic phenotype by interleukin-1 beta (IL-1 beta) requires an intermediate step involving signal transduction via the beta 1 family of integrin glycoproteins. |
| T3 |
409-614 |
Sentence |
denotes |
Recombinant human IL-1 beta inhibits human osteosarcoma cell proliferation, stimulates integrin expression, and induces alkaline phosphatase activity, a marker of osteoinductive and osteoblastic phenotype. |
| T4 |
615-838 |
Sentence |
denotes |
The approximately 10-fold stimulation of expression of the beta 1 integrins occurs rapidly (within 20 to 40 h), whereas the alkaline phosphatase activity is not induced until at least 5 days after the addition of IL-1 beta. |
| T5 |
839-1136 |
Sentence |
denotes |
To determine whether the early stimulation of integrin expression is required for the subsequent expression of alkaline phosphatase activity, polyclonal as well as monoclonal antibodies directed against the alpha 5 and beta 1 integrin subunits were added to cultures at the same time as IL-1 beta. |
| T6 |
1137-1402 |
Sentence |
denotes |
These antibodies inhibited by 55 to 82% the longer term induction of the osteoblastic differentiation marker, alkaline phosphatase activity, but did not however affect the IL-1 beta-induced stimulation of integrin expression or the inhibition of cell proliferation. |
| T7 |
1403-1501 |
Sentence |
denotes |
In addition, at the concentrations used, there was no effect of the antibodies on cell attachment. |
| T8 |
1502-1748 |
Sentence |
denotes |
These data suggest that the stimulation of integrin expression by IL-1 beta, and the resulting enhanced integrin-extracellular matrix interactions, is a required intermediate event in the IL-1 beta regulation of osteoblastic cell differentiation. |
| T9 |
1749-1960 |
Sentence |
denotes |
The data also suggest that the integrins are capable of signal transduction resulting in altered gene expression, and may also play a crucial role in modulating cytokine-mediated effects on cell differentiation. |
