PubMed:2776137 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/2776137","sourcedb":"PubMed","sourceid":"2776137","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/2776137","text":"Production and characterization of antisera raised to the manninotrionate group [alpha-D-Galp-(1----6)-alpha-D-Galp-(1----6)-D-gluconate].\nManninotrionate [alpha-D-Galp-(1----6)-alpha-D-Galp-(1----6)-D-gluconate] was coupled to bovine serum albumin (BSA), and antisera were raised in rabbits to this carbohydrate-protein conjugate. The antisera were studied by quantitative precipitation and hapten inhibition. Antisera against the manninotrionate-BSA conjugate showed the greatest reactivity with the homologous manninotrionate-BSA antigen, but also cross-reacted extensively with a manninotrionate-ovalbumin conjugate. The antisera gave weak precipitin reactions with beef-lung galactan, guaran, gum arabic, and larch arabinogalactan. Quantitative hapten-inhibition studies with a series of galactosyloligosaccharides indicated that the antisera possess a high degree of specificity toward terminal alpha-D-Galp-(1----6)-alpha-D-Galp units. The antisera did not agglutinate Ehrlich ascites tumor-cells, which have been demonstrated to contain cell-surface glycoproteins having alpha-D-galactosyl 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