PubMed:27694911 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/27694911","sourcedb":"PubMed","sourceid":"27694911","text":"Massively parallel single-nucleotide mutagenesis using reversibly terminated inosine.\nLarge-scale mutagenesis of target DNA sequences allows researchers to comprehensively assess the effects of single-nucleotide changes. Here we demonstrate the construction of a systematic allelic series (SAS) using massively parallel single-nucleotide mutagenesis with reversibly terminated deoxyinosine triphosphates (rtITP). We created a mutational library containing every possible single-nucleotide mutation surrounding the active site of the TEM-1 β-lactamase gene. When combined with high-throughput functional assays, SAS mutational libraries can expedite the functional assessment of genetic variation.","tracks":[{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":92,"end":97},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0002542"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"Anatomy-UBERON","color":"#e293ec","default":true}]}]}}