Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-90 |
Sentence |
denotes |
Antithrombin activity of a peptide corresponding to residues 54-75 of heparin cofactor II. |
T2 |
91-265 |
Sentence |
denotes |
Heparin cofactor II (HCII) is a highly specific serine proteinase inhibitor, which complexes covalently with thrombin in a reaction catalyzed by heparin and other polyanions. |
T3 |
266-428 |
Sentence |
denotes |
The molecular basis for the thrombin specificity may be explained by the identification here of a segment of HCII including residues 54-75 that binds to thrombin. |
T4 |
429-627 |
Sentence |
denotes |
A synthetic peptide, HCII(54-75), based on this segment of HCII, Gly-Glu-Glu-Asp-Asp-Asp-Tyr-Leu-Asp-Leu-Glu- Lys-Ile-Phe-Ala-Glu-Asp-Asp-Asp-Tyr-Ile-Asp inhibited thrombin's cleavage of fibrinogen. |
T5 |
628-708 |
Sentence |
denotes |
Clotting activity of thrombin was inhibited 50% at a concentration of 28 microM. |
T6 |
709-856 |
Sentence |
denotes |
Polyacrylamide gel electrophoresis showed that HCII(54-75) inhibited thrombin's cleavage of both the A alpha and B beta polypeptides in fibrinogen. |
T7 |
857-974 |
Sentence |
denotes |
However, the peptide did not block thrombin's active site, as hydrolysis of chromogenic substrates was not inhibited. |
T8 |
975-1108 |
Sentence |
denotes |
HCII(54-75) probably binds to the same site on thrombin as do carboxyl-terminal residues of hirudins, thrombin inhibitors of leeches. |
T9 |
1109-1306 |
Sentence |
denotes |
HCII(54-75) inhibited binding of thrombin to a synthetic peptide corresponding to residues 54-66 of hirudin PA, but the hirudin peptide was about 30-fold more potent in binding and clotting assays. |
T10 |
1307-1442 |
Sentence |
denotes |
Both synthetic peptides, as a result of their polyanionic character, might be expected to stimulate the reaction of HCII with thrombin. |
T11 |
1443-1586 |
Sentence |
denotes |
However, the hirudin-related peptide inhibited this reaction, suggesting that it blocked a site on thrombin required for interaction with HCII. |
T12 |
1587-1778 |
Sentence |
denotes |
HCII(54-75) had a net stimulatory effect on the thrombin-HCII reaction as a consequence of its lower affinity for thrombin and greater negative charge relative to the hirudin-related peptide. |
T13 |
1779-1965 |
Sentence |
denotes |
These studies suggest that residues 54-75 of HCII interact with a noncatalytic binding site on thrombin and that this interaction contributes to efficient inhibition of thrombin by HCII. |
T1 |
0-90 |
Sentence |
denotes |
Antithrombin activity of a peptide corresponding to residues 54-75 of heparin cofactor II. |
T2 |
91-265 |
Sentence |
denotes |
Heparin cofactor II (HCII) is a highly specific serine proteinase inhibitor, which complexes covalently with thrombin in a reaction catalyzed by heparin and other polyanions. |
T3 |
266-428 |
Sentence |
denotes |
The molecular basis for the thrombin specificity may be explained by the identification here of a segment of HCII including residues 54-75 that binds to thrombin. |
T4 |
429-627 |
Sentence |
denotes |
A synthetic peptide, HCII(54-75), based on this segment of HCII, Gly-Glu-Glu-Asp-Asp-Asp-Tyr-Leu-Asp-Leu-Glu- Lys-Ile-Phe-Ala-Glu-Asp-Asp-Asp-Tyr-Ile-Asp inhibited thrombin's cleavage of fibrinogen. |
T5 |
628-708 |
Sentence |
denotes |
Clotting activity of thrombin was inhibited 50% at a concentration of 28 microM. |
T6 |
709-856 |
Sentence |
denotes |
Polyacrylamide gel electrophoresis showed that HCII(54-75) inhibited thrombin's cleavage of both the A alpha and B beta polypeptides in fibrinogen. |
T7 |
857-974 |
Sentence |
denotes |
However, the peptide did not block thrombin's active site, as hydrolysis of chromogenic substrates was not inhibited. |
T8 |
975-1108 |
Sentence |
denotes |
HCII(54-75) probably binds to the same site on thrombin as do carboxyl-terminal residues of hirudins, thrombin inhibitors of leeches. |
T9 |
1109-1306 |
Sentence |
denotes |
HCII(54-75) inhibited binding of thrombin to a synthetic peptide corresponding to residues 54-66 of hirudin PA, but the hirudin peptide was about 30-fold more potent in binding and clotting assays. |
T10 |
1307-1442 |
Sentence |
denotes |
Both synthetic peptides, as a result of their polyanionic character, might be expected to stimulate the reaction of HCII with thrombin. |
T11 |
1443-1586 |
Sentence |
denotes |
However, the hirudin-related peptide inhibited this reaction, suggesting that it blocked a site on thrombin required for interaction with HCII. |
T12 |
1587-1778 |
Sentence |
denotes |
HCII(54-75) had a net stimulatory effect on the thrombin-HCII reaction as a consequence of its lower affinity for thrombin and greater negative charge relative to the hirudin-related peptide. |
T13 |
1779-1965 |
Sentence |
denotes |
These studies suggest that residues 54-75 of HCII interact with a noncatalytic binding site on thrombin and that this interaction contributes to efficient inhibition of thrombin by HCII. |