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PubMed:27551058 JSONTXT

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PubMed_ArguminSci

Id Subject Object Predicate Lexical cue
T1 78-211 DRI_Outcome denotes During morphogenesis, contraction of the actomyosin cytoskeleton within individual cells drives cell shape changes that fold tissues.
T2 212-302 DRI_Challenge denotes Coordination of cytoskeletal contractility is mediated by regulating RhoA GTPase activity.
T3 303-415 DRI_Approach denotes Guanine nucleotide exchange factors (GEFs) activate and GTPase-activating proteins (GAPs) inhibit RhoA activity.
T4 416-614 DRI_Background denotes Most studies of tissue folding, including apical constriction, have focused on how RhoA is activated by GEFs to promote cell contractility, with little investigation as to how GAPs may be important.
T5 615-825 DRI_Challenge denotes Here, we identify a critical role for a RhoA GAP, Cumberland GAP (C-GAP), which coordinates with a RhoA GEF, RhoGEF2, to organize spatiotemporal contractility during Drosophila melanogaster apical constriction.
T6 826-917 DRI_Approach denotes C-GAP spatially restricts RhoA pathway activity to a central position in the apical cortex.
T7 918-1055 DRI_Background denotes RhoGEF2 pulses precede myosin, and C-GAP is required for pulsation, suggesting that contractile pulses result from RhoA activity cycling.
T8 1056-1212 DRI_Challenge denotes Finally, C-GAP expression level influences the transition from reversible to irreversible cell shape change, which defines the onset of tissue shape change.
T9 1213-1338 DRI_Outcome denotes Our data demonstrate that RhoA activity cycling and modulating the ratio of RhoGEF2 to C-GAP are required for tissue folding.