PubMed:27268051
Annnotations
GlyCosmos15-Species
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| 4 | 58-68 | Species | denotes | Drosophila | 7227 |
| 26 | 517-527 | Species | denotes | Drosophila | 7227 |
| 42 | 1484-1494 | Species | denotes | Drosophila | 7227 |
GlyCosmos6-Glycan-Motif-Image
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 607-615 | Glycan_Motif | denotes | O-fucose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO |
| T2 | 642-649 | Glycan_Motif | denotes | glucose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG |
| T3 | 677-685 | Glycan_Motif | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G89565QL|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G49108TO |
| T5 | 837-845 | Glycan_Motif | denotes | O-fucose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO |
| T6 | 1017-1025 | Glycan_Motif | denotes | O-fucose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO |
| T7 | 1137-1144 | Glycan_Motif | denotes | glucose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG |
| T8 | 1205-1212 | Glycan_Motif | denotes | glucose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG |
| T9 | 1313-1321 | Glycan_Motif | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G89565QL|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G49108TO |
| T11 | 1364-1372 | Glycan_Motif | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G89565QL|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G49108TO |
GlyCosmos6-Glycan-Motif-Structure
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 607-615 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | O-fucose |
| T2 | 642-649 | https://glytoucan.org/Structures/Glycans/G15021LG | denotes | glucose |
| T3 | 677-685 | https://glytoucan.org/Structures/Glycans/G49108TO | denotes | O-GlcNAc |
| T4 | 677-685 | https://glytoucan.org/Structures/Glycans/G89565QL | denotes | O-GlcNAc |
| T5 | 837-845 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | O-fucose |
| T6 | 1017-1025 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | O-fucose |
| T7 | 1137-1144 | https://glytoucan.org/Structures/Glycans/G15021LG | denotes | glucose |
| T8 | 1205-1212 | https://glytoucan.org/Structures/Glycans/G15021LG | denotes | glucose |
| T9 | 1313-1321 | https://glytoucan.org/Structures/Glycans/G49108TO | denotes | O-GlcNAc |
| T10 | 1313-1321 | https://glytoucan.org/Structures/Glycans/G89565QL | denotes | O-GlcNAc |
| T11 | 1364-1372 | https://glytoucan.org/Structures/Glycans/G49108TO | denotes | O-GlcNAc |
| T12 | 1364-1372 | https://glytoucan.org/Structures/Glycans/G89565QL | denotes | O-GlcNAc |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-75 | Sentence | denotes | Mapping Sites of O-Glycosylation and Fringe Elongation on Drosophila Notch. |
| T2 | 76-192 | Sentence | denotes | Glycosylation of the Notch receptor is essential for its activity and serves as an important modulator of signaling. |
| T3 | 193-418 | Sentence | denotes | Three major forms of O-glycosylation are predicted to occur at consensus sites within the epidermal growth factor-like repeats in the extracellular domain of the receptor: O-fucosylation, O-glucosylation, and O-GlcNAcylation. |
| T4 | 419-692 | Sentence | denotes | We have performed comprehensive mass spectral analyses of these three types of O-glycosylation on Drosophila Notch produced in S2 cells and identified peptides containing all 22 predicted O-fucose sites, all 18 predicted O-glucose sites, and all 18 putative O-GlcNAc sites. |
| T5 | 693-816 | Sentence | denotes | Using semiquantitative mass spectral methods, we have evaluated the occupancy and relative amounts of glycans at each site. |
| T6 | 817-890 | Sentence | denotes | The majority of the O-fucose sites were modified to high stoichiometries. |
| T7 | 891-1120 | Sentence | denotes | Upon expression of the β3-N-acetylglucosaminyltransferase Fringe with Notch, we observed varying degrees of elongation beyond O-fucose monosaccharide, indicating that Fringe preferentially modifies certain sites more than others. |
| T8 | 1121-1231 | Sentence | denotes | Rumi modified O-glucose sites to high stoichiometries, although elongation of the O-glucose was site-specific. |
| T9 | 1232-1400 | Sentence | denotes | Although the current putative consensus sequence for O-GlcNAcylation predicts 18 O-GlcNAc sites on Notch, we only observed apparent O-GlcNAc modification at five sites. |
| T10 | 1401-1594 | Sentence | denotes | In addition, we performed mass spectral analysis on endogenous Notch purified from Drosophila embryos and found that the glycosylation states were similar to those found on Notch from S2 cells. |
| T11 | 1595-1735 | Sentence | denotes | These data provide foundational information for future studies investigating the mechanisms of how O-glycosylation regulates Notch activity. |
Glycosmos6-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glyco_epitope_db_id |
|---|---|---|---|---|---|
| T1 | 677-685 | GlycoEpitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T2 | 1313-1321 | GlycoEpitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T3 | 1364-1372 | GlycoEpitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
Glycosmos6-MAT
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 1495-1502 | http://purl.obolibrary.org/obo/MAT_0000226 | denotes | embryos |
NGLY1-deficiency
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| PD-NGLY1-deficiency-B_T1 | 679-685 | chem:24139 | denotes | GlcNAc |
| PD-NGLY1-deficiency-B_T2 | 1315-1321 | chem:24139 | denotes | GlcNAc |
| PD-NGLY1-deficiency-B_T3 | 1366-1372 | chem:24139 | denotes | GlcNAc |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 677-685 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
| T2 | 1313-1321 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
| T3 | 1364-1372 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 677-685 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
| T2 | 1313-1321 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
| T3 | 1364-1372 | Glycan | denotes | O-GlcNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G49108TO |
GlyCosmos-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 677-685 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T2 | 1313-1321 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T3 | 1364-1372 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
GlyCosmos15-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 283-299 | Body_part | denotes | epidermal growth | http://purl.obolibrary.org/obo/UBERON_0000021 |
| T2 | 327-340 | Body_part | denotes | extracellular | http://purl.obolibrary.org/obo/GO_0005576 |
GlyCosmos15-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 677-685 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T2 | 1313-1321 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
| T3 | 1364-1372 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | O-GlcNAc | http://www.glycoepitope.jp/epitopes/EP0004 |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-75 | Sentence | denotes | Mapping Sites of O-Glycosylation and Fringe Elongation on Drosophila Notch. |
| T2 | 76-192 | Sentence | denotes | Glycosylation of the Notch receptor is essential for its activity and serves as an important modulator of signaling. |
| T3 | 193-418 | Sentence | denotes | Three major forms of O-glycosylation are predicted to occur at consensus sites within the epidermal growth factor-like repeats in the extracellular domain of the receptor: O-fucosylation, O-glucosylation, and O-GlcNAcylation. |
| T4 | 419-692 | Sentence | denotes | We have performed comprehensive mass spectral analyses of these three types of O-glycosylation on Drosophila Notch produced in S2 cells and identified peptides containing all 22 predicted O-fucose sites, all 18 predicted O-glucose sites, and all 18 putative O-GlcNAc sites. |
| T5 | 693-816 | Sentence | denotes | Using semiquantitative mass spectral methods, we have evaluated the occupancy and relative amounts of glycans at each site. |
| T6 | 817-890 | Sentence | denotes | The majority of the O-fucose sites were modified to high stoichiometries. |
| T7 | 891-1120 | Sentence | denotes | Upon expression of the β3-N-acetylglucosaminyltransferase Fringe with Notch, we observed varying degrees of elongation beyond O-fucose monosaccharide, indicating that Fringe preferentially modifies certain sites more than others. |
| T8 | 1121-1231 | Sentence | denotes | Rumi modified O-glucose sites to high stoichiometries, although elongation of the O-glucose was site-specific. |
| T9 | 1232-1400 | Sentence | denotes | Although the current putative consensus sequence for O-GlcNAcylation predicts 18 O-GlcNAc sites on Notch, we only observed apparent O-GlcNAc modification at five sites. |
| T10 | 1401-1594 | Sentence | denotes | In addition, we performed mass spectral analysis on endogenous Notch purified from Drosophila embryos and found that the glycosylation states were similar to those found on Notch from S2 cells. |
| T11 | 1595-1735 | Sentence | denotes | These data provide foundational information for future studies investigating the mechanisms of how O-glycosylation regulates Notch activity. |
GlyCosmos15-FMA
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 69-74 | Body_part | denotes | Notch | FMA:75036 |
| T2 | 97-102 | Body_part | denotes | Notch | FMA:75036 |
| T3 | 528-533 | Body_part | denotes | Notch | FMA:75036 |
| T4 | 961-966 | Body_part | denotes | Notch | FMA:75036 |
| T5 | 1331-1336 | Body_part | denotes | Notch | FMA:75036 |
| T6 | 1464-1469 | Body_part | denotes | Notch | FMA:75036 |
| T7 | 1495-1502 | Body_part | denotes | embryos | FMA:296970 |
| T8 | 1574-1579 | Body_part | denotes | Notch | FMA:75036 |
| T9 | 1720-1725 | Body_part | denotes | Notch | FMA:75036 |
GlyCosmos15-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 1495-1502 | Body_part | denotes | embryos | http://purl.obolibrary.org/obo/MAT_0000226 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 58-68 | OrganismTaxon | denotes | Drosophila | 2081351|32281|7215 |
| T4 | 517-527 | OrganismTaxon | denotes | Drosophila | 2081351|32281|7215 |
| T7 | 1484-1494 | OrganismTaxon | denotes | Drosophila | 2081351|32281|7215 |
Anatomy-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 1495-1502 | Body_part | denotes | embryos | http://purl.obolibrary.org/obo/MAT_0000226 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 283-299 | Body_part | denotes | epidermal growth | http://purl.obolibrary.org/obo/UBERON_0000021 |
| T2 | 327-340 | Body_part | denotes | extracellular | http://purl.obolibrary.org/obo/GO_0005576 |