PubMed:27090809
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/27090809","sourcedb":"PubMed","sourceid":"27090809","text":"Stimulation of orphan nuclear receptor HR38 gene expression by PTTH in prothoracic glands of the silkworm, Bombyx mori.\nA complex signaling network appears to be involved in prothoracicotropic hormone (PTTH)-stimulated ecdysteroidogenesis in insect prothoracic glands (PGs). Less is known about the genomic action of PTTH signaling. In the present study, we investigated the effect of PTTH on the expression of Bombyx mori HR38, an immediate early gene (IEG) identified in insect systems. Our results showed that treatment of B. mori PGs with PTTH in vitro resulted in a rapid increase in HR38 expression. Injection of PTTH into day-5 last instar larvae also greatly increased HR38 expression, verifying the in vitro effect. Cycloheximide did not affect induction of HR38 expression, suggesting that protein synthesis is not required for PTTH's effect. A mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) kinase (MEK) inhibitor (U0126), and a phosphoinositide 3-kinase (PI3K) inhibitor (LY294002), partially inhibited PTTH-stimulated HR38 expression, implying the involvement of both the ERK and PI3K signaling pathways. When PGs were treated with agents that directly elevate the intracellular Ca(2+) concentration (either A23187 or thapsigargin), an increase in HR38 expression was also detected, indicating that Ca(2+) is involved in PTTH-stimulated HR38 gene expression. A Western blot analysis showed that PTTH treatment increased the HR38 protein level, and protein levels showed a dramatic increase during the later stages of the last larval instar. Expression of HR38 transcription in response to PTTH appeared to undergo development-specific changes. Treatment with ecdysone in vitro did not affect HR38 expression. However, 20-hydroxyecdysone treatment decreased HR38 expression. Taken together, these results demonstrate that HR38 is a PTTH-stimulated IEG that is, at least in part, induced through Ca(2+)/ERK and PI3K signaling. The present study proposes a potential cross talk mechanism between PTTH and ecdysone signaling to regulate insect development and lays a foundation for a better understanding of the mechanisms of PTTH's actions.","tracks":[{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":39,"end":43},"obj":"Gene:693064"},{"id":"T2","span":{"begin":63,"end":67},"obj":"Gene:692767"},{"id":"T3","span":{"begin":97,"end":105},"obj":"Species:7091"},{"id":"T4","span":{"begin":107,"end":118},"obj":"Species:7091"},{"id":"T5","span":{"begin":202,"end":206},"obj":"Gene:692767"},{"id":"T6","span":{"begin":269,"end":272},"obj":"Chemical:-"},{"id":"T7","span":{"begin":317,"end":321},"obj":"Gene:692767"},{"id":"T8","span":{"begin":385,"end":389},"obj":"Gene:692767"},{"id":"T9","span":{"begin":411,"end":422},"obj":"Species:7091"},{"id":"T10","span":{"begin":423,"end":427},"obj":"Gene:693064"},{"id":"T11","span":{"begin":526,"end":533},"obj":"Species:7091"},{"id":"T12","span":{"begin":543,"end":547},"obj":"Gene:692767"},{"id":"T13","span":{"begin":589,"end":593},"obj":"Gene:693064"},{"id":"T14","span":{"begin":619,"end":623},"obj":"Gene:692767"},{"id":"T15","span":{"begin":677,"end":681},"obj":"Gene:693064"},{"id":"T16","span":{"begin":725,"end":738},"obj":"Chemical:MESH:D003513"},{"id":"T17","span":{"begin":767,"end":771},"obj":"Gene:693064"},{"id":"T18","span":{"begin":838,"end":842},"obj":"Gene:692767"},{"id":"T19","span":{"begin":934,"end":937},"obj":"Gene:692466"},{"id":"T20","span":{"begin":947,"end":950},"obj":"Gene:692467"},{"id":"T21","span":{"begin":963,"end":968},"obj":"Chemical:MESH:C113580"},{"id":"T22","span":{"begin":1021,"end":1029},"obj":"Chemical:MESH:C085911"},{"id":"T23","span":{"begin":1052,"end":1056},"obj":"Gene:692767"},{"id":"T24","span":{"begin":1068,"end":1072},"obj":"Gene:693064"},{"id":"T25","span":{"begin":1122,"end":1125},"obj":"Gene:692466"},{"id":"T26","span":{"begin":1229,"end":1235},"obj":"Chemical:MESH:D000069285"},{"id":"T27","span":{"begin":1258,"end":1264},"obj":"Chemical:MESH:D000001"},{"id":"T28","span":{"begin":1268,"end":1280},"obj":"Chemical:MESH:D019284"},{"id":"T29","span":{"begin":1298,"end":1302},"obj":"Gene:693064"},{"id":"T30","span":{"begin":1349,"end":1355},"obj":"Chemical:MESH:D000069285"},{"id":"T31","span":{"begin":1371,"end":1375},"obj":"Gene:692767"},{"id":"T32","span":{"begin":1387,"end":1391},"obj":"Gene:693064"},{"id":"T33","span":{"begin":1445,"end":1449},"obj":"Gene:692767"},{"id":"T34","span":{"begin":1474,"end":1478},"obj":"Gene:693064"},{"id":"T35","span":{"begin":1605,"end":1609},"obj":"Gene:693064"},{"id":"T36","span":{"begin":1639,"end":1643},"obj":"Gene:692767"},{"id":"T37","span":{"begin":1709,"end":1717},"obj":"Chemical:MESH:D004440"},{"id":"T38","span":{"begin":1742,"end":1746},"obj":"Gene:693064"},{"id":"T39","span":{"begin":1768,"end":1786},"obj":"Chemical:MESH:D004441"},{"id":"T40","span":{"begin":1807,"end":1811},"obj":"Gene:693064"},{"id":"T41","span":{"begin":1871,"end":1875},"obj":"Gene:693064"},{"id":"T42","span":{"begin":1881,"end":1885},"obj":"Gene:692767"},{"id":"T43","span":{"begin":1944,"end":1950},"obj":"Chemical:MESH:D000069285"},{"id":"T44","span":{"begin":1951,"end":1954},"obj":"Gene:692466"},{"id":"T45","span":{"begin":2043,"end":2047},"obj":"Gene:692767"},{"id":"T46","span":{"begin":2172,"end":2176},"obj":"Gene:692767"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkworm"},{"subj":"T2","pred":"source","obj":"silkworm"},{"subj":"T3","pred":"source","obj":"silkworm"},{"subj":"T4","pred":"source","obj":"silkworm"},{"subj":"T5","pred":"source","obj":"silkworm"},{"subj":"T6","pred":"source","obj":"silkworm"},{"subj":"T7","pred":"source","obj":"silkworm"},{"subj":"T8","pred":"source","obj":"silkworm"},{"subj":"T9","pred":"source","obj":"silkworm"},{"subj":"T10","pred":"source","obj":"silkworm"},{"subj":"T11","pred":"source","obj":"silkworm"},{"subj":"T12","pred":"source","obj":"silkworm"},{"subj":"T13","pred":"source","obj":"silkworm"},{"subj":"T14","pred":"source","obj":"silkworm"},{"subj":"T15","pred":"source","obj":"silkworm"},{"subj":"T16","pred":"source","obj":"silkworm"},{"subj":"T17","pred":"source","obj":"silkworm"},{"subj":"T18","pred":"source","obj":"silkworm"},{"subj":"T19","pred":"source","obj":"silkworm"},{"subj":"T20","pred":"source","obj":"silkworm"},{"subj":"T21","pred":"source","obj":"silkworm"},{"subj":"T22","pred":"source","obj":"silkworm"},{"subj":"T23","pred":"source","obj":"silkworm"},{"subj":"T24","pred":"source","obj":"silkworm"},{"subj":"T25","pred":"source","obj":"silkworm"},{"subj":"T26","pred":"source","obj":"silkworm"},{"subj":"T27","pred":"source","obj":"silkworm"},{"subj":"T28","pred":"source","obj":"silkworm"},{"subj":"T29","pred":"source","obj":"silkworm"},{"subj":"T30","pred":"source","obj":"silkworm"},{"subj":"T31","pred":"source","obj":"silkworm"},{"subj":"T32","pred":"source","obj":"silkworm"},{"subj":"T33","pred":"source","obj":"silkworm"},{"subj":"T34","pred":"source","obj":"silkworm"},{"subj":"T35","pred":"source","obj":"silkworm"},{"subj":"T36","pred":"source","obj":"silkworm"},{"subj":"T37","pred":"source","obj":"silkworm"},{"subj":"T38","pred":"source","obj":"silkworm"},{"subj":"T39","pred":"source","obj":"silkworm"},{"subj":"T40","pred":"source","obj":"silkworm"},{"subj":"T41","pred":"source","obj":"silkworm"},{"subj":"T42","pred":"source","obj":"silkworm"},{"subj":"T43","pred":"source","obj":"silkworm"},{"subj":"T44","pred":"source","obj":"silkworm"},{"subj":"T45","pred":"source","obj":"silkworm"},{"subj":"T46","pred":"source","obj":"silkworm"}]},{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":39,"end":43},"obj":"Gene:693064"},{"id":"T10","span":{"begin":423,"end":427},"obj":"Gene:693064"},{"id":"T11","span":{"begin":526,"end":533},"obj":"Species:7091"},{"id":"T12","span":{"begin":543,"end":547},"obj":"Gene:692767"},{"id":"T13","span":{"begin":589,"end":593},"obj":"Gene:693064"},{"id":"T14","span":{"begin":619,"end":623},"obj":"Gene:692767"},{"id":"T15","span":{"begin":677,"end":681},"obj":"Gene:693064"},{"id":"T16","span":{"begin":725,"end":738},"obj":"Chemical:MESH:D003513"},{"id":"T17","span":{"begin":767,"end":771},"obj":"Gene:693064"},{"id":"T18","span":{"begin":838,"end":842},"obj":"Gene:692767"},{"id":"T19","span":{"begin":934,"end":937},"obj":"Gene:692466"},{"id":"T2","span":{"begin":63,"end":67},"obj":"Gene:692767"},{"id":"T20","span":{"begin":947,"end":950},"obj":"Gene:692467"},{"id":"T21","span":{"begin":963,"end":968},"obj":"Chemical:MESH:C113580"},{"id":"T22","span":{"begin":1021,"end":1029},"obj":"Chemical:MESH:C085911"},{"id":"T23","span":{"begin":1052,"end":1056},"obj":"Gene:692767"},{"id":"T24","span":{"begin":1068,"end":1072},"obj":"Gene:693064"},{"id":"T25","span":{"begin":1122,"end":1125},"obj":"Gene:692466"},{"id":"T26","span":{"begin":1229,"end":1235},"obj":"Chemical:MESH:D000069285"},{"id":"T27","span":{"begin":1258,"end":1264},"obj":"Chemical:MESH:D000001"},{"id":"T28","span":{"begin":1268,"end":1280},"obj":"Chemical:MESH:D019284"},{"id":"T29","span":{"begin":1298,"end":1302},"obj":"Gene:693064"},{"id":"T3","span":{"begin":97,"end":105},"obj":"Species:7091"},{"id":"T30","span":{"begin":1349,"end":1355},"obj":"Chemical:MESH:D000069285"},{"id":"T31","span":{"begin":1371,"end":1375},"obj":"Gene:692767"},{"id":"T32","span":{"begin":1387,"end":1391},"obj":"Gene:693064"},{"id":"T33","span":{"begin":1445,"end":1449},"obj":"Gene:692767"},{"id":"T34","span":{"begin":1474,"end":1478},"obj":"Gene:693064"},{"id":"T35","span":{"begin":1605,"end":1609},"obj":"Gene:693064"},{"id":"T36","span":{"begin":1639,"end":1643},"obj":"Gene:692767"},{"id":"T37","span":{"begin":1709,"end":1717},"obj":"Chemical:MESH:D004440"},{"id":"T38","span":{"begin":1742,"end":1746},"obj":"Gene:693064"},{"id":"T39","span":{"begin":1768,"end":1786},"obj":"Chemical:MESH:D004441"},{"id":"T4","span":{"begin":107,"end":118},"obj":"Species:7091"},{"id":"T40","span":{"begin":1807,"end":1811},"obj":"Gene:693064"},{"id":"T41","span":{"begin":1871,"end":1875},"obj":"Gene:693064"},{"id":"T42","span":{"begin":1881,"end":1885},"obj":"Gene:692767"},{"id":"T43","span":{"begin":1944,"end":1950},"obj":"Chemical:MESH:D000069285"},{"id":"T44","span":{"begin":1951,"end":1954},"obj":"Gene:692466"},{"id":"T45","span":{"begin":2043,"end":2047},"obj":"Gene:692767"},{"id":"T46","span":{"begin":2172,"end":2176},"obj":"Gene:692767"},{"id":"T5","span":{"begin":202,"end":206},"obj":"Gene:692767"},{"id":"T6","span":{"begin":269,"end":272},"obj":"Chemical:-"},{"id":"T7","span":{"begin":317,"end":321},"obj":"Gene:692767"},{"id":"T8","span":{"begin":385,"end":389},"obj":"Gene:692767"},{"id":"T9","span":{"begin":411,"end":422},"obj":"Species:7091"},{"id":"T47","span":{"begin":63,"end":67},"obj":"BMO_00114"},{"id":"T51","span":{"begin":174,"end":200},"obj":"BMO_00114"},{"id":"T52","span":{"begin":202,"end":206},"obj":"BMO_00114"},{"id":"T53","span":{"begin":317,"end":321},"obj":"BMO_00114"},{"id":"T54","span":{"begin":385,"end":389},"obj":"BMO_00114"},{"id":"T55","span":{"begin":543,"end":547},"obj":"BMO_00114"},{"id":"T56","span":{"begin":619,"end":623},"obj":"BMO_00114"},{"id":"T57","span":{"begin":838,"end":844},"obj":"BMO_00114"},{"id":"T58","span":{"begin":1052,"end":1056},"obj":"BMO_00114"},{"id":"T59","span":{"begin":1371,"end":1375},"obj":"BMO_00114"},{"id":"T60","span":{"begin":1445,"end":1449},"obj":"BMO_00114"},{"id":"T61","span":{"begin":1639,"end":1643},"obj":"BMO_00114"},{"id":"T62","span":{"begin":1881,"end":1885},"obj":"BMO_00114"},{"id":"T63","span":{"begin":2043,"end":2047},"obj":"BMO_00114"},{"id":"T64","span":{"begin":2172,"end":2178},"obj":"BMO_00114"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkwormbase"},{"subj":"T10","pred":"source","obj":"silkwormbase"},{"subj":"T11","pred":"source","obj":"silkwormbase"},{"subj":"T12","pred":"source","obj":"silkwormbase"},{"subj":"T13","pred":"source","obj":"silkwormbase"},{"subj":"T14","pred":"source","obj":"silkwormbase"},{"subj":"T15","pred":"source","obj":"silkwormbase"},{"subj":"T16","pred":"source","obj":"silkwormbase"},{"subj":"T17","pred":"source","obj":"silkwormbase"},{"subj":"T18","pred":"source","obj":"silkwormbase"},{"subj":"T19","pred":"source","obj":"silkwormbase"},{"subj":"T2","pred":"source","obj":"silkwormbase"},{"subj":"T20","pred":"source","obj":"silkwormbase"},{"subj":"T21","pred":"source","obj":"silkwormbase"},{"subj":"T22","pred":"source","obj":"silkwormbase"},{"subj":"T23","pred":"source","obj":"silkwormbase"},{"subj":"T24","pred":"source","obj":"silkwormbase"},{"subj":"T25","pred":"source","obj":"silkwormbase"},{"subj":"T26","pred":"source","obj":"silkwormbase"},{"subj":"T27","pred":"source","obj":"silkwormbase"},{"subj":"T28","pred":"source","obj":"silkwormbase"},{"subj":"T29","pred":"source","obj":"silkwormbase"},{"subj":"T3","pred":"source","obj":"silkwormbase"},{"subj":"T30","pred":"source","obj":"silkwormbase"},{"subj":"T31","pred":"source","obj":"silkwormbase"},{"subj":"T32","pred":"source","obj":"silkwormbase"},{"subj":"T33","pred":"source","obj":"silkwormbase"},{"subj":"T34","pred":"source","obj":"silkwormbase"},{"subj":"T35","pred":"source","obj":"silkwormbase"},{"subj":"T36","pred":"source","obj":"silkwormbase"},{"subj":"T37","pred":"source","obj":"silkwormbase"},{"subj":"T38","pred":"source","obj":"silkwormbase"},{"subj":"T39","pred":"source","obj":"silkwormbase"},{"subj":"T4","pred":"source","obj":"silkwormbase"},{"subj":"T40","pred":"source","obj":"silkwormbase"},{"subj":"T41","pred":"source","obj":"silkwormbase"},{"subj":"T42","pred":"source","obj":"silkwormbase"},{"subj":"T43","pred":"source","obj":"silkwormbase"},{"subj":"T44","pred":"source","obj":"silkwormbase"},{"subj":"T45","pred":"source","obj":"silkwormbase"},{"subj":"T46","pred":"source","obj":"silkwormbase"},{"subj":"T5","pred":"source","obj":"silkwormbase"},{"subj":"T6","pred":"source","obj":"silkwormbase"},{"subj":"T7","pred":"source","obj":"silkwormbase"},{"subj":"T8","pred":"source","obj":"silkwormbase"},{"subj":"T9","pred":"source","obj":"silkwormbase"},{"subj":"T47","pred":"source","obj":"silkwormbase"},{"subj":"T51","pred":"source","obj":"silkwormbase"},{"subj":"T52","pred":"source","obj":"silkwormbase"},{"subj":"T53","pred":"source","obj":"silkwormbase"},{"subj":"T54","pred":"source","obj":"silkwormbase"},{"subj":"T55","pred":"source","obj":"silkwormbase"},{"subj":"T56","pred":"source","obj":"silkwormbase"},{"subj":"T57","pred":"source","obj":"silkwormbase"},{"subj":"T58","pred":"source","obj":"silkwormbase"},{"subj":"T59","pred":"source","obj":"silkwormbase"},{"subj":"T60","pred":"source","obj":"silkwormbase"},{"subj":"T61","pred":"source","obj":"silkwormbase"},{"subj":"T62","pred":"source","obj":"silkwormbase"},{"subj":"T63","pred":"source","obj":"silkwormbase"},{"subj":"T64","pred":"source","obj":"silkwormbase"}]},{"project":"silkworm_phenotype","denotations":[{"id":"T64","span":{"begin":2172,"end":2178},"obj":"BMO_00114"},{"id":"T63","span":{"begin":2043,"end":2047},"obj":"BMO_00114"},{"id":"T62","span":{"begin":1881,"end":1885},"obj":"BMO_00114"},{"id":"T61","span":{"begin":1639,"end":1643},"obj":"BMO_00114"},{"id":"T60","span":{"begin":1445,"end":1449},"obj":"BMO_00114"},{"id":"T59","span":{"begin":1371,"end":1375},"obj":"BMO_00114"},{"id":"T58","span":{"begin":1052,"end":1056},"obj":"BMO_00114"},{"id":"T57","span":{"begin":838,"end":844},"obj":"BMO_00114"},{"id":"T56","span":{"begin":619,"end":623},"obj":"BMO_00114"},{"id":"T55","span":{"begin":543,"end":547},"obj":"BMO_00114"},{"id":"T54","span":{"begin":385,"end":389},"obj":"BMO_00114"},{"id":"T53","span":{"begin":317,"end":321},"obj":"BMO_00114"},{"id":"T52","span":{"begin":202,"end":206},"obj":"BMO_00114"},{"id":"T51","span":{"begin":174,"end":200},"obj":"BMO_00114"},{"id":"T47","span":{"begin":63,"end":67},"obj":"BMO_00114"},{"id":"T9","span":{"begin":411,"end":422},"obj":"Species:7091"},{"id":"T8","span":{"begin":385,"end":389},"obj":"Gene:692767"},{"id":"T7","span":{"begin":317,"end":321},"obj":"Gene:692767"},{"id":"T6","span":{"begin":269,"end":272},"obj":"Chemical:-"},{"id":"T5","span":{"begin":202,"end":206},"obj":"Gene:692767"},{"id":"T46","span":{"begin":2172,"end":2176},"obj":"Gene:692767"},{"id":"T45","span":{"begin":2043,"end":2047},"obj":"Gene:692767"},{"id":"T44","span":{"begin":1951,"end":1954},"obj":"Gene:692466"},{"id":"T43","span":{"begin":1944,"end":1950},"obj":"Chemical:MESH:D000069285"},{"id":"T42","span":{"begin":1881,"end":1885},"obj":"Gene:692767"},{"id":"T41","span":{"begin":1871,"end":1875},"obj":"Gene:693064"},{"id":"T40","span":{"begin":1807,"end":1811},"obj":"Gene:693064"},{"id":"T4","span":{"begin":107,"end":118},"obj":"Species:7091"},{"id":"T39","span":{"begin":1768,"end":1786},"obj":"Chemical:MESH:D004441"},{"id":"T38","span":{"begin":1742,"end":1746},"obj":"Gene:693064"},{"id":"T37","span":{"begin":1709,"end":1717},"obj":"Chemical:MESH:D004440"},{"id":"T36","span":{"begin":1639,"end":1643},"obj":"Gene:692767"},{"id":"T35","span":{"begin":1605,"end":1609},"obj":"Gene:693064"},{"id":"T34","span":{"begin":1474,"end":1478},"obj":"Gene:693064"},{"id":"T33","span":{"begin":1445,"end":1449},"obj":"Gene:692767"},{"id":"T32","span":{"begin":1387,"end":1391},"obj":"Gene:693064"},{"id":"T31","span":{"begin":1371,"end":1375},"obj":"Gene:692767"},{"id":"T30","span":{"begin":1349,"end":1355},"obj":"Chemical:MESH:D000069285"},{"id":"T3","span":{"begin":97,"end":105},"obj":"Species:7091"},{"id":"T29","span":{"begin":1298,"end":1302},"obj":"Gene:693064"},{"id":"T28","span":{"begin":1268,"end":1280},"obj":"Chemical:MESH:D019284"},{"id":"T27","span":{"begin":1258,"end":1264},"obj":"Chemical:MESH:D000001"},{"id":"T26","span":{"begin":1229,"end":1235},"obj":"Chemical:MESH:D000069285"},{"id":"T25","span":{"begin":1122,"end":1125},"obj":"Gene:692466"},{"id":"T24","span":{"begin":1068,"end":1072},"obj":"Gene:693064"},{"id":"T23","span":{"begin":1052,"end":1056},"obj":"Gene:692767"},{"id":"T22","span":{"begin":1021,"end":1029},"obj":"Chemical:MESH:C085911"},{"id":"T21","span":{"begin":963,"end":968},"obj":"Chemical:MESH:C113580"},{"id":"T20","span":{"begin":947,"end":950},"obj":"Gene:692467"},{"id":"T2","span":{"begin":63,"end":67},"obj":"Gene:692767"},{"id":"T19","span":{"begin":934,"end":937},"obj":"Gene:692466"},{"id":"T18","span":{"begin":838,"end":842},"obj":"Gene:692767"},{"id":"T17","span":{"begin":767,"end":771},"obj":"Gene:693064"},{"id":"T16","span":{"begin":725,"end":738},"obj":"Chemical:MESH:D003513"},{"id":"T15","span":{"begin":677,"end":681},"obj":"Gene:693064"},{"id":"T14","span":{"begin":619,"end":623},"obj":"Gene:692767"},{"id":"T13","span":{"begin":589,"end":593},"obj":"Gene:693064"},{"id":"T12","span":{"begin":543,"end":547},"obj":"Gene:692767"},{"id":"T11","span":{"begin":526,"end":533},"obj":"Species:7091"},{"id":"T10","span":{"begin":423,"end":427},"obj":"Gene:693064"},{"id":"T1","span":{"begin":39,"end":43},"obj":"Gene:693064"}],"attributes":[{"subj":"T64","pred":"source","obj":"silkworm_phenotype"},{"subj":"T63","pred":"source","obj":"silkworm_phenotype"},{"subj":"T62","pred":"source","obj":"silkworm_phenotype"},{"subj":"T61","pred":"source","obj":"silkworm_phenotype"},{"subj":"T60","pred":"source","obj":"silkworm_phenotype"},{"subj":"T59","pred":"source","obj":"silkworm_phenotype"},{"subj":"T58","pred":"source","obj":"silkworm_phenotype"},{"subj":"T57","pred":"source","obj":"silkworm_phenotype"},{"subj":"T56","pred":"source","obj":"silkworm_phenotype"},{"subj":"T55","pred":"source","obj":"silkworm_phenotype"},{"subj":"T54","pred":"source","obj":"silkworm_phenotype"},{"subj":"T53","pred":"source","obj":"silkworm_phenotype"},{"subj":"T52","pred":"source","obj":"silkworm_phenotype"},{"subj":"T51","pred":"source","obj":"silkworm_phenotype"},{"subj":"T47","pred":"source","obj":"silkworm_phenotype"},{"subj":"T9","pred":"source","obj":"silkworm_phenotype"},{"subj":"T8","pred":"source","obj":"silkworm_phenotype"},{"subj":"T7","pred":"source","obj":"silkworm_phenotype"},{"subj":"T6","pred":"source","obj":"silkworm_phenotype"},{"subj":"T5","pred":"source","obj":"silkworm_phenotype"},{"subj":"T46","pred":"source","obj":"silkworm_phenotype"},{"subj":"T45","pred":"source","obj":"silkworm_phenotype"},{"subj":"T44","pred":"source","obj":"silkworm_phenotype"},{"subj":"T43","pred":"source","obj":"silkworm_phenotype"},{"subj":"T42","pred":"source","obj":"silkworm_phenotype"},{"subj":"T41","pred":"source","obj":"silkworm_phenotype"},{"subj":"T40","pred":"source","obj":"silkworm_phenotype"},{"subj":"T4","pred":"source","obj":"silkworm_phenotype"},{"subj":"T39","pred":"source","obj":"silkworm_phenotype"},{"subj":"T38","pred":"source","obj":"silkworm_phenotype"},{"subj":"T37","pred":"source","obj":"silkworm_phenotype"},{"subj":"T36","pred":"source","obj":"silkworm_phenotype"},{"subj":"T35","pred":"source","obj":"silkworm_phenotype"},{"subj":"T34","pred":"source","obj":"silkworm_phenotype"},{"subj":"T33","pred":"source","obj":"silkworm_phenotype"},{"subj":"T32","pred":"source","obj":"silkworm_phenotype"},{"subj":"T31","pred":"source","obj":"silkworm_phenotype"},{"subj":"T30","pred":"source","obj":"silkworm_phenotype"},{"subj":"T3","pred":"source","obj":"silkworm_phenotype"},{"subj":"T29","pred":"source","obj":"silkworm_phenotype"},{"subj":"T28","pred":"source","obj":"silkworm_phenotype"},{"subj":"T27","pred":"source","obj":"silkworm_phenotype"},{"subj":"T26","pred":"source","obj":"silkworm_phenotype"},{"subj":"T25","pred":"source","obj":"silkworm_phenotype"},{"subj":"T24","pred":"source","obj":"silkworm_phenotype"},{"subj":"T23","pred":"source","obj":"silkworm_phenotype"},{"subj":"T22","pred":"source","obj":"silkworm_phenotype"},{"subj":"T21","pred":"source","obj":"silkworm_phenotype"},{"subj":"T20","pred":"source","obj":"silkworm_phenotype"},{"subj":"T2","pred":"source","obj":"silkworm_phenotype"},{"subj":"T19","pred":"source","obj":"silkworm_phenotype"},{"subj":"T18","pred":"source","obj":"silkworm_phenotype"},{"subj":"T17","pred":"source","obj":"silkworm_phenotype"},{"subj":"T16","pred":"source","obj":"silkworm_phenotype"},{"subj":"T15","pred":"source","obj":"silkworm_phenotype"},{"subj":"T14","pred":"source","obj":"silkworm_phenotype"},{"subj":"T13","pred":"source","obj":"silkworm_phenotype"},{"subj":"T12","pred":"source","obj":"silkworm_phenotype"},{"subj":"T11","pred":"source","obj":"silkworm_phenotype"},{"subj":"T10","pred":"source","obj":"silkworm_phenotype"},{"subj":"T1","pred":"source","obj":"silkworm_phenotype"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"silkworm","color":"#df93ec","default":true},{"id":"silkwormbase","color":"#93ecc5"},{"id":"silkworm_phenotype","color":"#ecab93"}]}]}}