| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-123 |
Sentence |
denotes |
The acidic C-terminus of vaccinia virus I3 single-strand binding protein promotes proper assembly of DNA-protein complexes. |
| TextSentencer_T2 |
124-289 |
Sentence |
denotes |
The vaccinia virus I3L gene encodes a single-stranded DNA binding protein (SSB) that is essential for virus DNA replication and is conserved in all Chordopoxviruses. |
| TextSentencer_T3 |
290-384 |
Sentence |
denotes |
The I3 protein contains a negatively charged C-terminal tail that is a common feature of SSBs. |
| TextSentencer_T4 |
385-472 |
Sentence |
denotes |
Such acidic tails are critical for SSB-dependent replication, recombination and repair. |
| TextSentencer_T5 |
473-644 |
Sentence |
denotes |
We cloned and purified variants of the I3 protein, along with a homolog from molluscum contagiosum virus, and tested how the acidic tail affected DNA-protein interactions. |
| TextSentencer_T6 |
645-832 |
Sentence |
denotes |
Deleting the C terminus of I3 enhanced the affinity for single-stranded DNA cellulose and gel shift analyses showed that it also altered the migration of I3-DNA complexes in agarose gels. |
| TextSentencer_T7 |
833-945 |
Sentence |
denotes |
Microinjecting an antibody against I3 into vaccinia-infected cells also selectively inhibited virus replication. |
| TextSentencer_T8 |
946-1097 |
Sentence |
denotes |
We suggest that this domain promotes cooperative binding of I3 to DNA in a way that would maintain an open DNA configuration around a replication site. |
| T1 |
0-123 |
Sentence |
denotes |
The acidic C-terminus of vaccinia virus I3 single-strand binding protein promotes proper assembly of DNA-protein complexes. |
| T2 |
124-289 |
Sentence |
denotes |
The vaccinia virus I3L gene encodes a single-stranded DNA binding protein (SSB) that is essential for virus DNA replication and is conserved in all Chordopoxviruses. |
| T3 |
290-384 |
Sentence |
denotes |
The I3 protein contains a negatively charged C-terminal tail that is a common feature of SSBs. |
| T4 |
385-472 |
Sentence |
denotes |
Such acidic tails are critical for SSB-dependent replication, recombination and repair. |
| T5 |
473-644 |
Sentence |
denotes |
We cloned and purified variants of the I3 protein, along with a homolog from molluscum contagiosum virus, and tested how the acidic tail affected DNA-protein interactions. |
| T6 |
645-832 |
Sentence |
denotes |
Deleting the C terminus of I3 enhanced the affinity for single-stranded DNA cellulose and gel shift analyses showed that it also altered the migration of I3-DNA complexes in agarose gels. |
| T7 |
833-945 |
Sentence |
denotes |
Microinjecting an antibody against I3 into vaccinia-infected cells also selectively inhibited virus replication. |
| T8 |
946-1097 |
Sentence |
denotes |
We suggest that this domain promotes cooperative binding of I3 to DNA in a way that would maintain an open DNA configuration around a replication site. |