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PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 101-1500 UNLABELLED denotes RNA interference offers effective control of several economically important insect pests. Bemisia tabaci is an important field crop pest, which causes significant yield loss worldwide. In our earlier study, we have demonstrated successful control of B. tabaci through transgenic plant mediated RNAi. However, selection of target genes without off-target effect(s) has been major concern so far and therefore, a critical exploration for B. tabaci specific targets is frantically required. In this study, we have followed proteomics approach to discover B. tabaci specific targets for RNAi and identified unique nucleotide sequences in functional genes (n=11) of the pest. For this, we have developed proteome profile of B. tabaci extract using two-dimensional electrophoresis. A total of 504 protein spots were analyzed on mass-spectrometer and 453 proteins including 246 non-redundant proteins have been identified successfully. Complementation of the proteome data with available nucleotide database has helped us to interpret the unique nucleotide sequences. These nucleotide stretches may serve as environmentally safe targets for RNAi mediated control of the pest through crop genetic engineering. To the best of our knowledge, it is the most complete proteome of any whitefly species. We have also demonstrated application of proteomics in the identification of functional transcripts for RNAi.
T2 1526-2594 UNASSIGNED denotes Insects cause major loss to crop productivity through direct and indirect damages. Among them, hemipteran group of insects are major contributor of global crop yield loss. In current study, gel based proteome profile of B. tabaci (one of the major hemipteran crop insect pest) is developed and characterized, which is a gap area in field of whitefly biology. It is an important data set of future whitefly studies like insect-plant interaction, virulence of whiteflies, their control program and discovery of new pesticides. Out of various control strategies, RNA interference offers a great potential to combat the whitefly successfully. However, the uniqueness of target genes and off target impact of the technology remains a challenge to scientific community. We used our proteome data set for the identification of B. tabaci specific gene targets for RNAi mediated control. The identified genes are critical for the life cycle of B. tabaci hence, could be proven as good molecules for making transgenic crop plant for efficient control of whiteflies in the field.

sentences

Id Subject Object Predicate Lexical cue
T1 0-88 Sentence denotes Proteome analysis of Bemisia tabaci suggests specific targets for RNAi mediated control.
T2 89-190 Sentence denotes UNLABELLED: RNA interference offers effective control of several economically important insect pests.
T3 191-285 Sentence denotes Bemisia tabaci is an important field crop pest, which causes significant yield loss worldwide.
T4 286-400 Sentence denotes In our earlier study, we have demonstrated successful control of B. tabaci through transgenic plant mediated RNAi.
T5 401-588 Sentence denotes However, selection of target genes without off-target effect(s) has been major concern so far and therefore, a critical exploration for B. tabaci specific targets is frantically required.
T6 589-771 Sentence denotes In this study, we have followed proteomics approach to discover B. tabaci specific targets for RNAi and identified unique nucleotide sequences in functional genes (n=11) of the pest.
T7 772-876 Sentence denotes For this, we have developed proteome profile of B. tabaci extract using two-dimensional electrophoresis.
T8 877-1029 Sentence denotes A total of 504 protein spots were analyzed on mass-spectrometer and 453 proteins including 246 non-redundant proteins have been identified successfully.
T9 1030-1161 Sentence denotes Complementation of the proteome data with available nucleotide database has helped us to interpret the unique nucleotide sequences.
T10 1162-1302 Sentence denotes These nucleotide stretches may serve as environmentally safe targets for RNAi mediated control of the pest through crop genetic engineering.
T11 1303-1390 Sentence denotes To the best of our knowledge, it is the most complete proteome of any whitefly species.
T12 1391-1500 Sentence denotes We have also demonstrated application of proteomics in the identification of functional transcripts for RNAi.
T13 1501-1525 Sentence denotes BIOLOGICAL SIGNIFICANCE:
T14 1526-1608 Sentence denotes Insects cause major loss to crop productivity through direct and indirect damages.
T15 1609-1697 Sentence denotes Among them, hemipteran group of insects are major contributor of global crop yield loss.
T16 1698-1884 Sentence denotes In current study, gel based proteome profile of B. tabaci (one of the major hemipteran crop insect pest) is developed and characterized, which is a gap area in field of whitefly biology.
T17 1885-2050 Sentence denotes It is an important data set of future whitefly studies like insect-plant interaction, virulence of whiteflies, their control program and discovery of new pesticides.
T18 2051-2164 Sentence denotes Out of various control strategies, RNA interference offers a great potential to combat the whitefly successfully.
T19 2165-2289 Sentence denotes However, the uniqueness of target genes and off target impact of the technology remains a challenge to scientific community.
T20 2290-2404 Sentence denotes We used our proteome data set for the identification of B. tabaci specific gene targets for RNAi mediated control.
T21 2405-2594 Sentence denotes The identified genes are critical for the life cycle of B. tabaci hence, could be proven as good molecules for making transgenic crop plant for efficient control of whiteflies in the field.
T1 0-88 Sentence denotes Proteome analysis of Bemisia tabaci suggests specific targets for RNAi mediated control.
T2 89-190 Sentence denotes UNLABELLED: RNA interference offers effective control of several economically important insect pests.
T3 191-285 Sentence denotes Bemisia tabaci is an important field crop pest, which causes significant yield loss worldwide.
T4 286-400 Sentence denotes In our earlier study, we have demonstrated successful control of B. tabaci through transgenic plant mediated RNAi.
T5 401-588 Sentence denotes However, selection of target genes without off-target effect(s) has been major concern so far and therefore, a critical exploration for B. tabaci specific targets is frantically required.
T6 589-771 Sentence denotes In this study, we have followed proteomics approach to discover B. tabaci specific targets for RNAi and identified unique nucleotide sequences in functional genes (n=11) of the pest.
T7 772-876 Sentence denotes For this, we have developed proteome profile of B. tabaci extract using two-dimensional electrophoresis.
T8 877-1029 Sentence denotes A total of 504 protein spots were analyzed on mass-spectrometer and 453 proteins including 246 non-redundant proteins have been identified successfully.
T9 1030-1161 Sentence denotes Complementation of the proteome data with available nucleotide database has helped us to interpret the unique nucleotide sequences.
T10 1162-1302 Sentence denotes These nucleotide stretches may serve as environmentally safe targets for RNAi mediated control of the pest through crop genetic engineering.
T11 1303-1390 Sentence denotes To the best of our knowledge, it is the most complete proteome of any whitefly species.
T12 1391-1500 Sentence denotes We have also demonstrated application of proteomics in the identification of functional transcripts for RNAi.
T13 1501-1525 Sentence denotes BIOLOGICAL SIGNIFICANCE:
T14 1526-1608 Sentence denotes Insects cause major loss to crop productivity through direct and indirect damages.
T15 1609-1697 Sentence denotes Among them, hemipteran group of insects are major contributor of global crop yield loss.
T16 1698-1884 Sentence denotes In current study, gel based proteome profile of B. tabaci (one of the major hemipteran crop insect pest) is developed and characterized, which is a gap area in field of whitefly biology.
T17 1885-2050 Sentence denotes It is an important data set of future whitefly studies like insect-plant interaction, virulence of whiteflies, their control program and discovery of new pesticides.
T18 2051-2164 Sentence denotes Out of various control strategies, RNA interference offers a great potential to combat the whitefly successfully.
T19 2165-2289 Sentence denotes However, the uniqueness of target genes and off target impact of the technology remains a challenge to scientific community.
T20 2290-2404 Sentence denotes We used our proteome data set for the identification of B. tabaci specific gene targets for RNAi mediated control.
T21 2405-2594 Sentence denotes The identified genes are critical for the life cycle of B. tabaci hence, could be proven as good molecules for making transgenic crop plant for efficient control of whiteflies in the field.

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 233-237 Disease denotes pest http://purl.obolibrary.org/obo/MONDO_0019095
T2 766-770 Disease denotes pest http://purl.obolibrary.org/obo/MONDO_0019095
T3 1264-1268 Disease denotes pest http://purl.obolibrary.org/obo/MONDO_0019095
T4 1797-1801 Disease denotes pest http://purl.obolibrary.org/obo/MONDO_0019095

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 21-35 OrganismTaxon denotes Bemisia tabaci 7038
T2 191-205 OrganismTaxon denotes Bemisia tabaci 7038

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 900-905 Body_part denotes spots http://purl.obolibrary.org/obo/UBERON_2002283