| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-131 |
Sentence |
denotes |
Size product modulation by enzyme concentration reveals two distinct levan elongation mechanisms in Bacillus subtilis levansucrase. |
| TextSentencer_T2 |
132-355 |
Sentence |
denotes |
Two levan distributions are produced typically by Bacillus subtilis levansucrase (SacB): a high-molecular weight (HMW) levan with an average molecular weight of 2300 kDa, and a low-molecular weight (LMW) levan with 7.2 kDa. |
| TextSentencer_T3 |
356-460 |
Sentence |
denotes |
Previous results have demonstrated how reaction conditions modulate levan molecular weight distribution. |
| TextSentencer_T4 |
461-653 |
Sentence |
denotes |
Here we demonstrate that the SacB enzyme is able to perform two mechanisms: a processive mechanism for the synthesis of HMW levan and a non-processive mechanism for the synthesis of LMW levan. |
| TextSentencer_T5 |
654-756 |
Sentence |
denotes |
Furthermore, the effect of enzyme and substrate concentration on the elongation mechanism was studied. |
| TextSentencer_T6 |
757-902 |
Sentence |
denotes |
While a negligible effect of substrate concentration was observed, we found that SacB elongation mechanism is determined by enzyme concentration. |
| TextSentencer_T7 |
903-1115 |
Sentence |
denotes |
A high concentration of enzyme is required to synthesize LMW levan, involving the sequential formation of a wide variety of intermediate size levan oligosaccharides with a degree of polymerization (DP) up to ∼70. |
| TextSentencer_T8 |
1116-1292 |
Sentence |
denotes |
In contrast, an HMW levan distribution is synthesized through a processive mechanism producing oligosaccharides with DP <20, in reactions occurring at low enzyme concentration. |
| TextSentencer_T9 |
1293-1610 |
Sentence |
denotes |
Additionally, reactions where levansucrase concentration was varied while the total enzyme activity was kept constant (using a combination of active SacB and an inactive SacB E342A/D86A) allowed us to demonstrate that enzyme concentration and not enzyme activity affects the final levan molecular weight distribution. |
| TextSentencer_T10 |
1611-1780 |
Sentence |
denotes |
The effect of enzyme concentration on the elongation mechanism is discussed in detail, finding that protein-product interactions are responsible for the mechanism shift. |
| T5 |
654-756 |
Sentence |
denotes |
Furthermore, the effect of enzyme and substrate concentration on the elongation mechanism was studied. |
| T6 |
757-902 |
Sentence |
denotes |
While a negligible effect of substrate concentration was observed, we found that SacB elongation mechanism is determined by enzyme concentration. |
| T7 |
903-1115 |
Sentence |
denotes |
A high concentration of enzyme is required to synthesize LMW levan, involving the sequential formation of a wide variety of intermediate size levan oligosaccharides with a degree of polymerization (DP) up to ∼70. |
| T8 |
1116-1292 |
Sentence |
denotes |
In contrast, an HMW levan distribution is synthesized through a processive mechanism producing oligosaccharides with DP <20, in reactions occurring at low enzyme concentration. |
| T9 |
1293-1610 |
Sentence |
denotes |
Additionally, reactions where levansucrase concentration was varied while the total enzyme activity was kept constant (using a combination of active SacB and an inactive SacB E342A/D86A) allowed us to demonstrate that enzyme concentration and not enzyme activity affects the final levan molecular weight distribution. |
| T10 |
1611-1780 |
Sentence |
denotes |
The effect of enzyme concentration on the elongation mechanism is discussed in detail, finding that protein-product interactions are responsible for the mechanism shift. |
| T1 |
0-131 |
Sentence |
denotes |
Size product modulation by enzyme concentration reveals two distinct levan elongation mechanisms in Bacillus subtilis levansucrase. |
| T2 |
132-355 |
Sentence |
denotes |
Two levan distributions are produced typically by Bacillus subtilis levansucrase (SacB): a high-molecular weight (HMW) levan with an average molecular weight of 2300 kDa, and a low-molecular weight (LMW) levan with 7.2 kDa. |
| T3 |
356-460 |
Sentence |
denotes |
Previous results have demonstrated how reaction conditions modulate levan molecular weight distribution. |
| T4 |
461-653 |
Sentence |
denotes |
Here we demonstrate that the SacB enzyme is able to perform two mechanisms: a processive mechanism for the synthesis of HMW levan and a non-processive mechanism for the synthesis of LMW levan. |
| T1 |
0-131 |
Sentence |
denotes |
Size product modulation by enzyme concentration reveals two distinct levan elongation mechanisms in Bacillus subtilis levansucrase. |
| T2 |
132-355 |
Sentence |
denotes |
Two levan distributions are produced typically by Bacillus subtilis levansucrase (SacB): a high-molecular weight (HMW) levan with an average molecular weight of 2300 kDa, and a low-molecular weight (LMW) levan with 7.2 kDa. |
| T3 |
356-460 |
Sentence |
denotes |
Previous results have demonstrated how reaction conditions modulate levan molecular weight distribution. |
| T4 |
461-653 |
Sentence |
denotes |
Here we demonstrate that the SacB enzyme is able to perform two mechanisms: a processive mechanism for the synthesis of HMW levan and a non-processive mechanism for the synthesis of LMW levan. |
| T5 |
654-756 |
Sentence |
denotes |
Furthermore, the effect of enzyme and substrate concentration on the elongation mechanism was studied. |
| T6 |
757-902 |
Sentence |
denotes |
While a negligible effect of substrate concentration was observed, we found that SacB elongation mechanism is determined by enzyme concentration. |
| T7 |
903-1115 |
Sentence |
denotes |
A high concentration of enzyme is required to synthesize LMW levan, involving the sequential formation of a wide variety of intermediate size levan oligosaccharides with a degree of polymerization (DP) up to ∼70. |
| T8 |
1116-1292 |
Sentence |
denotes |
In contrast, an HMW levan distribution is synthesized through a processive mechanism producing oligosaccharides with DP <20, in reactions occurring at low enzyme concentration. |
| T9 |
1293-1610 |
Sentence |
denotes |
Additionally, reactions where levansucrase concentration was varied while the total enzyme activity was kept constant (using a combination of active SacB and an inactive SacB E342A/D86A) allowed us to demonstrate that enzyme concentration and not enzyme activity affects the final levan molecular weight distribution. |
| T10 |
1611-1780 |
Sentence |
denotes |
The effect of enzyme concentration on the elongation mechanism is discussed in detail, finding that protein-product interactions are responsible for the mechanism shift. |
