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PubMed:26603318 JSONTXT

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Glycan-Motif

Id Subject Object Predicate Lexical cue
T1 107-114 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T2 520-527 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T3 1212-1219 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 107-114 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ
T2 520-527 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ
T3 1212-1219 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 107-114 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T2 520-527 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T3 1212-1219 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-42 Sentence denotes Stability of Curcuma longa rhizome lectin:
TextSentencer_T2 43-74 Sentence denotes Role of N-linked glycosylation.
TextSentencer_T3 75-248 Sentence denotes Curcuma longa rhizome lectin, a mannose-binding protein of non-seed portions of turmeric, is known to have antifungal, antibacterial and α-glucosidase inhibitory activities.
TextSentencer_T4 249-409 Sentence denotes We studied the role of complex-type glycans attached to asparagine (Asn) 66 and Asn 110 to elucidate the role of carbohydrates in lectin activity and stability.
TextSentencer_T5 410-720 Sentence denotes Apart from the native lectin, the characteristics of a deglycosylated Escherichia coli expressed lectin, high-mannose oligosaccharides at both asparagines and its glycosylation mutants N66Q and N110Q expressed in Pichia pastoris, were compared to understand the relationship between glycosylation and activity.
TextSentencer_T6 721-974 Sentence denotes Far UV circular dichroism (CD) spectra, fluorescence emission maximum, hemagglutination assay show no change in secondary or tertiary structures or sugar-binding properties between wild-type and aforementioned recombinant lectins under physiological pH.
TextSentencer_T7 975-1122 Sentence denotes But reduced agglutination activity and loss of tertiary structure are observed in the acidic pH range for the deglycosylated and the N110Q protein.
TextSentencer_T8 1123-1412 Sentence denotes In thermal and guanidine hydrochloride (GdnCl)-induced unfolding, the wild-type and high-mannose lectins possess higher stability compared with the deglycosylated recombinant lectin and both mutants, as measured by a higher Tm of denaturation or a greater free energy change, respectively.
TextSentencer_T9 1413-1631 Sentence denotes Reversibility experiments after thermal denaturation reveal that deglycosylated proteins tend to aggregate during thermal inactivation but the wild type shows a much greater recovery to the native state upon refolding.
TextSentencer_T10 1632-1909 Sentence denotes These results suggest that N-glycosylation in turmeric lectin is important for the maintenance of its proper folding upon changes in pH, and that the oligosaccharides help in maintaining the active conformation and prevent aggregation in unfolded or partially folded molecules.
T1 0-42 Sentence denotes Stability of Curcuma longa rhizome lectin:
T2 43-74 Sentence denotes Role of N-linked glycosylation.
T3 75-248 Sentence denotes Curcuma longa rhizome lectin, a mannose-binding protein of non-seed portions of turmeric, is known to have antifungal, antibacterial and α-glucosidase inhibitory activities.
T4 249-409 Sentence denotes We studied the role of complex-type glycans attached to asparagine (Asn) 66 and Asn 110 to elucidate the role of carbohydrates in lectin activity and stability.
T5 410-720 Sentence denotes Apart from the native lectin, the characteristics of a deglycosylated Escherichia coli expressed lectin, high-mannose oligosaccharides at both asparagines and its glycosylation mutants N66Q and N110Q expressed in Pichia pastoris, were compared to understand the relationship between glycosylation and activity.
T6 721-974 Sentence denotes Far UV circular dichroism (CD) spectra, fluorescence emission maximum, hemagglutination assay show no change in secondary or tertiary structures or sugar-binding properties between wild-type and aforementioned recombinant lectins under physiological pH.
T7 975-1122 Sentence denotes But reduced agglutination activity and loss of tertiary structure are observed in the acidic pH range for the deglycosylated and the N110Q protein.
T8 1123-1412 Sentence denotes In thermal and guanidine hydrochloride (GdnCl)-induced unfolding, the wild-type and high-mannose lectins possess higher stability compared with the deglycosylated recombinant lectin and both mutants, as measured by a higher Tm of denaturation or a greater free energy change, respectively.
T9 1413-1631 Sentence denotes Reversibility experiments after thermal denaturation reveal that deglycosylated proteins tend to aggregate during thermal inactivation but the wild type shows a much greater recovery to the native state upon refolding.
T10 1632-1909 Sentence denotes These results suggest that N-glycosylation in turmeric lectin is important for the maintenance of its proper folding upon changes in pH, and that the oligosaccharides help in maintaining the active conformation and prevent aggregation in unfolded or partially folded molecules.
T1 0-42 Sentence denotes Stability of Curcuma longa rhizome lectin:
T2 43-74 Sentence denotes Role of N-linked glycosylation.
T3 75-248 Sentence denotes Curcuma longa rhizome lectin, a mannose-binding protein of non-seed portions of turmeric, is known to have antifungal, antibacterial and α-glucosidase inhibitory activities.
T4 249-409 Sentence denotes We studied the role of complex-type glycans attached to asparagine (Asn) 66 and Asn 110 to elucidate the role of carbohydrates in lectin activity and stability.
T5 410-720 Sentence denotes Apart from the native lectin, the characteristics of a deglycosylated Escherichia coli expressed lectin, high-mannose oligosaccharides at both asparagines and its glycosylation mutants N66Q and N110Q expressed in Pichia pastoris, were compared to understand the relationship between glycosylation and activity.
T6 721-974 Sentence denotes Far UV circular dichroism (CD) spectra, fluorescence emission maximum, hemagglutination assay show no change in secondary or tertiary structures or sugar-binding properties between wild-type and aforementioned recombinant lectins under physiological pH.
T7 975-1122 Sentence denotes But reduced agglutination activity and loss of tertiary structure are observed in the acidic pH range for the deglycosylated and the N110Q protein.
T8 1123-1412 Sentence denotes In thermal and guanidine hydrochloride (GdnCl)-induced unfolding, the wild-type and high-mannose lectins possess higher stability compared with the deglycosylated recombinant lectin and both mutants, as measured by a higher Tm of denaturation or a greater free energy change, respectively.
T9 1413-1631 Sentence denotes Reversibility experiments after thermal denaturation reveal that deglycosylated proteins tend to aggregate during thermal inactivation but the wild type shows a much greater recovery to the native state upon refolding.
T10 1632-1909 Sentence denotes These results suggest that N-glycosylation in turmeric lectin is important for the maintenance of its proper folding upon changes in pH, and that the oligosaccharides help in maintaining the active conformation and prevent aggregation in unfolded or partially folded molecules.

GlycoBiology-PACDB

Id Subject Object Predicate Lexical cue
_T1 480-496 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC002,LEC056,LEC062,LEC069,LEC081,LEC111,LEC133,LEC171,LEC177,LEC187,LEC211,LEC242,LEC252,LEC258,LEC259,LEC260,LEC262,LEC369,LEC377,LEC422,LEC442,LEC448,LEC450,LEC451,LEC454,LEC472,LEC492,LEC620 denotes Escherichia coli
_T2 480-496 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC157,LEC407 denotes Escherichia coli
_T3 480-496 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC754 denotes Escherichia coli
_T4 480-496 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC243,LEC640 denotes Escherichia coli
_T5 480-496 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC295,LEC417 denotes Escherichia coli
_T6 480-496 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC487 denotes Escherichia coli
_T7 480-496 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC244,LEC256,LEC354 denotes Escherichia coli
_T8 480-496 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC054,LEC058,LEC073,LEC082,LEC091,LEC103,LEC109,LEC110,LEC123,LEC158,LEC179,LEC198,LEC205,LEC222,LEC223,LEC224,LEC225,LEC232,LEC298,LEC357,LEC378,LEC383,LEC388,LEC389,LEC397,LEC401,LEC410,LEC452 denotes Escherichia coli
_T9 480-496 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC636 denotes Escherichia coli

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 107-114 FMAID:82801 denotes mannose
_T2 107-114 FMAID:196796 denotes mannose
_T3 123-130 FMAID:67257 denotes protein
_T4 123-130 FMAID:165447 denotes protein
_T5 305-315 FMAID:82750 denotes asparagine
_T6 305-315 FMAID:196739 denotes asparagine
_T7 362-375 FMAID:82737 denotes carbohydrates
_T8 362-375 FMAID:197276 denotes carbohydrates
_T9 520-527 FMAID:196796 denotes mannose
_T10 520-527 FMAID:82801 denotes mannose
_T11 528-544 FMAID:196731 denotes oligosaccharides
_T12 528-544 FMAID:82742 denotes oligosaccharides
_T13 553-564 FMAID:82750 denotes asparagines
_T14 553-564 FMAID:196739 denotes asparagines
_T15 869-874 FMAID:196724 denotes sugar
_T16 1114-1121 FMAID:67257 denotes protein
_T17 1114-1121 FMAID:165447 denotes protein
_T18 1138-1147 FMAID:82775 denotes guanidine
_T19 1138-1147 FMAID:196768 denotes guanidine
_T20 1212-1219 FMAID:196796 denotes mannose
_T21 1212-1219 FMAID:82801 denotes mannose
_T22 1493-1501 FMAID:165447 denotes proteins
_T23 1493-1501 FMAID:67257 denotes proteins
_T24 1782-1798 FMAID:196731 denotes oligosaccharides
_T25 1782-1798 FMAID:82742 denotes oligosaccharides
_T26 1807-1818 FMAID:214734 denotes maintaining

uniprot-human

Id Subject Object Predicate Lexical cue
T1 97-122 http://www.uniprot.org/uniprot/Q9UQG6 denotes lectin, a mannose-binding
T2 97-122 http://www.uniprot.org/uniprot/Q12907 denotes lectin, a mannose-binding
T3 97-122 http://www.uniprot.org/uniprot/Q96TF8 denotes lectin, a mannose-binding
T4 212-225 http://www.uniprot.org/uniprot/O43451 denotes α-glucosidase

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 13-20 http://purl.bioontology.org/ontology/NCBITAXON/99568 denotes Curcuma
T2 13-26 http://purl.bioontology.org/ontology/NCBITAXON/136217 denotes Curcuma longa
T3 75-82 http://purl.bioontology.org/ontology/NCBITAXON/99568 denotes Curcuma
T4 75-88 http://purl.bioontology.org/ontology/NCBITAXON/136217 denotes Curcuma longa
T5 134-137 http://purl.bioontology.org/ontology/NCBITAXON/604139 denotes non
T6 480-491 http://purl.bioontology.org/ontology/NCBITAXON/561 denotes Escherichia
T7 623-629 http://purl.bioontology.org/ontology/NCBITAXON/1365831 denotes Pichia
T8 1126-1133 http://purl.bioontology.org/ontology/NCBITAXON/68933 denotes thermal
T9 1445-1452 http://purl.bioontology.org/ontology/NCBITAXON/68933 denotes thermal
T10 1527-1534 http://purl.bioontology.org/ontology/NCBITAXON/68933 denotes thermal

GO-BP

Id Subject Object Predicate Lexical cue
T1 60-73 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T2 573-586 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T3 693-706 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T4 1661-1674 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T5 987-1000 http://purl.obolibrary.org/obo/GO_0000752 denotes agglutination
T6 987-1000 http://purl.obolibrary.org/obo/GO_0007157 denotes agglutination
T7 1478-1501 http://purl.obolibrary.org/obo/GO_0006517 denotes deglycosylated proteins

GO-MF

Id Subject Object Predicate Lexical cue
T1 97-122 http://purl.obolibrary.org/obo/GO_0005537 denotes lectin, a mannose-binding
T2 107-122 http://purl.obolibrary.org/obo/GO_0005537 denotes mannose-binding
T3 115-122 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T4 875-882 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T5 115-122 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T6 875-882 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T7 115-122 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T8 875-882 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T9 115-122 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T10 875-882 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T11 115-130 http://purl.obolibrary.org/obo/GO_0005515 denotes binding protein
T12 869-882 http://purl.obolibrary.org/obo/GO_0030246 denotes sugar-binding

GlycoBiology-Motifs

Id Subject Object Predicate Lexical cue
T1 515-527 http://rdf.glycoinfo.org/glycan/G00028MO denotes high-mannose
T2 1207-1219 http://rdf.glycoinfo.org/glycan/G00028MO denotes high-mannose

GlyTouCan-IUPAC

Id Subject Object Predicate Lexical cue
GlycanIUPAC_T1 134-137 "http://rdf.glycoinfo.org/glycan/G02780QX" denotes non
GlycanIUPAC_T2 134-137 "http://rdf.glycoinfo.org/glycan/G18425DX" denotes non
GlycanIUPAC_T3 134-137 "http://rdf.glycoinfo.org/glycan/G18630JE" denotes non
GlycanIUPAC_T4 134-137 "http://rdf.glycoinfo.org/glycan/G01004IT" denotes non
GlycanIUPAC_T5 134-137 "http://rdf.glycoinfo.org/glycan/G87301QZ" denotes non
GlycanIUPAC_T6 134-137 "http://rdf.glycoinfo.org/glycan/G39790GW" denotes non
GlycanIUPAC_T7 134-137 "http://rdf.glycoinfo.org/glycan/G42928BB" denotes non
GlycanIUPAC_T8 134-137 "http://rdf.glycoinfo.org/glycan/G51134HC" denotes non
GlycanIUPAC_T9 134-137 "http://rdf.glycoinfo.org/glycan/G68183GR" denotes non
GlycanIUPAC_T10 134-137 "http://rdf.glycoinfo.org/glycan/G46883FA" denotes non
GlycanIUPAC_T11 134-137 "http://rdf.glycoinfo.org/glycan/G54702VY" denotes non
GlycanIUPAC_T12 869-874 "http://rdf.glycoinfo.org/glycan/G59665TO" denotes sugar
GlycanIUPAC_T13 869-874 "http://rdf.glycoinfo.org/glycan/G32915EI" denotes sugar
GlycanIUPAC_T14 869-874 "http://rdf.glycoinfo.org/glycan/G60625TS" denotes sugar

Lectin-Jamboree-Sentence

Id Subject Object Predicate Lexical cue
T1 0-42 Sentence denotes Stability of Curcuma longa rhizome lectin:
T2 43-74 Sentence denotes Role of N-linked glycosylation.
T3 75-248 Sentence denotes Curcuma longa rhizome lectin, a mannose-binding protein of non-seed portions of turmeric, is known to have antifungal, antibacterial and α-glucosidase inhibitory activities.
T4 249-409 Sentence denotes We studied the role of complex-type glycans attached to asparagine (Asn) 66 and Asn 110 to elucidate the role of carbohydrates in lectin activity and stability.
T5 410-720 Sentence denotes Apart from the native lectin, the characteristics of a deglycosylated Escherichia coli expressed lectin, high-mannose oligosaccharides at both asparagines and its glycosylation mutants N66Q and N110Q expressed in Pichia pastoris, were compared to understand the relationship between glycosylation and activity.
T6 721-974 Sentence denotes Far UV circular dichroism (CD) spectra, fluorescence emission maximum, hemagglutination assay show no change in secondary or tertiary structures or sugar-binding properties between wild-type and aforementioned recombinant lectins under physiological pH.
T7 975-1122 Sentence denotes But reduced agglutination activity and loss of tertiary structure are observed in the acidic pH range for the deglycosylated and the N110Q protein.
T8 1123-1412 Sentence denotes In thermal and guanidine hydrochloride (GdnCl)-induced unfolding, the wild-type and high-mannose lectins possess higher stability compared with the deglycosylated recombinant lectin and both mutants, as measured by a higher Tm of denaturation or a greater free energy change, respectively.
T9 1413-1631 Sentence denotes Reversibility experiments after thermal denaturation reveal that deglycosylated proteins tend to aggregate during thermal inactivation but the wild type shows a much greater recovery to the native state upon refolding.
T10 1632-1909 Sentence denotes These results suggest that N-glycosylation in turmeric lectin is important for the maintenance of its proper folding upon changes in pH, and that the oligosaccharides help in maintaining the active conformation and prevent aggregation in unfolded or partially folded molecules.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 13-26 OrganismTaxon denotes Curcuma longa 136217
T2 75-88 OrganismTaxon denotes Curcuma longa 136217
T3 480-496 OrganismTaxon denotes Escherichia coli 562
T4 623-638 OrganismTaxon denotes Pichia pastoris 4922