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sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-113 Sentence denotes Purification and characterization of a Shigella conjugate vaccine, produced by glycoengineering Escherichia coli.
TextSentencer_T2 114-252 Sentence denotes Shigellosis remains a major cause of diarrheal disease in developing countries and causes substantial morbidity and mortality in children.
TextSentencer_T3 253-434 Sentence denotes Glycoconjugate vaccines consisting of bacterial surface polysaccharides conjugated to carrier proteins are the most effective vaccines for controlling invasive bacterial infections.
TextSentencer_T4 435-714 Sentence denotes Nevertheless, the development of a multivalent conjugate vaccine to prevent Shigellosis has been hampered by the complex manufacturing process as the surface polysaccharide for each strain requires extraction, hydrolysis, chemical activation and conjugation to a carrier protein.
TextSentencer_T5 715-850 Sentence denotes The use of an innovative biosynthetic Escherichia coli glycosylation system substantially simplifies the production of glycoconjugates.
TextSentencer_T6 851-1047 Sentence denotes Herein, the Shigella dysenteriae type 1 (Sd1) O-polysaccharide is expressed and its functional assembly on an E. coli glycosyl carrier lipid is demonstrated by HPLC analysis and mass spectrometry.
TextSentencer_T7 1048-1274 Sentence denotes The polysaccharide is enzymatically conjugated to specific asparagine residues of the carrier protein by co-expression of the PglB oligosaccharyltransferase and the carrier protein exotoxin A (EPA) from Pseudomonas aeruginosa.
TextSentencer_T8 1275-1469 Sentence denotes The extraction and purification of the Shigella glycoconjugate (Sd1-EPA) and its detailed characterization by the use of physicochemical methods including NMR and mass spectrometry is described.
TextSentencer_T9 1470-1668 Sentence denotes The report shows for the first time that bioconjugation provides a newly developed and improved approach to produce an Sd1 glycoconjugate that can be characterized using state-of-the-art techniques.
TextSentencer_T10 1669-1870 Sentence denotes In addition, this generic process together with the analytical methods is ideally suited for the production of additional Shigella serotypes, allowing the development of a multivalent Shigella vaccine.
T1 0-113 Sentence denotes Purification and characterization of a Shigella conjugate vaccine, produced by glycoengineering Escherichia coli.
T2 114-252 Sentence denotes Shigellosis remains a major cause of diarrheal disease in developing countries and causes substantial morbidity and mortality in children.
T3 253-434 Sentence denotes Glycoconjugate vaccines consisting of bacterial surface polysaccharides conjugated to carrier proteins are the most effective vaccines for controlling invasive bacterial infections.
T4 435-714 Sentence denotes Nevertheless, the development of a multivalent conjugate vaccine to prevent Shigellosis has been hampered by the complex manufacturing process as the surface polysaccharide for each strain requires extraction, hydrolysis, chemical activation and conjugation to a carrier protein.
T5 715-850 Sentence denotes The use of an innovative biosynthetic Escherichia coli glycosylation system substantially simplifies the production of glycoconjugates.
T6 851-1047 Sentence denotes Herein, the Shigella dysenteriae type 1 (Sd1) O-polysaccharide is expressed and its functional assembly on an E. coli glycosyl carrier lipid is demonstrated by HPLC analysis and mass spectrometry.
T7 1048-1274 Sentence denotes The polysaccharide is enzymatically conjugated to specific asparagine residues of the carrier protein by co-expression of the PglB oligosaccharyltransferase and the carrier protein exotoxin A (EPA) from Pseudomonas aeruginosa.
T8 1275-1469 Sentence denotes The extraction and purification of the Shigella glycoconjugate (Sd1-EPA) and its detailed characterization by the use of physicochemical methods including NMR and mass spectrometry is described.
T9 1470-1668 Sentence denotes The report shows for the first time that bioconjugation provides a newly developed and improved approach to produce an Sd1 glycoconjugate that can be characterized using state-of-the-art techniques.
T10 1669-1870 Sentence denotes In addition, this generic process together with the analytical methods is ideally suited for the production of additional Shigella serotypes, allowing the development of a multivalent Shigella vaccine.
T1 0-113 Sentence denotes Purification and characterization of a Shigella conjugate vaccine, produced by glycoengineering Escherichia coli.
T2 114-252 Sentence denotes Shigellosis remains a major cause of diarrheal disease in developing countries and causes substantial morbidity and mortality in children.
T3 253-434 Sentence denotes Glycoconjugate vaccines consisting of bacterial surface polysaccharides conjugated to carrier proteins are the most effective vaccines for controlling invasive bacterial infections.
T4 435-714 Sentence denotes Nevertheless, the development of a multivalent conjugate vaccine to prevent Shigellosis has been hampered by the complex manufacturing process as the surface polysaccharide for each strain requires extraction, hydrolysis, chemical activation and conjugation to a carrier protein.
T5 715-850 Sentence denotes The use of an innovative biosynthetic Escherichia coli glycosylation system substantially simplifies the production of glycoconjugates.
T6 851-1047 Sentence denotes Herein, the Shigella dysenteriae type 1 (Sd1) O-polysaccharide is expressed and its functional assembly on an E. coli glycosyl carrier lipid is demonstrated by HPLC analysis and mass spectrometry.
T7 1048-1274 Sentence denotes The polysaccharide is enzymatically conjugated to specific asparagine residues of the carrier protein by co-expression of the PglB oligosaccharyltransferase and the carrier protein exotoxin A (EPA) from Pseudomonas aeruginosa.
T8 1275-1469 Sentence denotes The extraction and purification of the Shigella glycoconjugate (Sd1-EPA) and its detailed characterization by the use of physicochemical methods including NMR and mass spectrometry is described.
T9 1470-1668 Sentence denotes The report shows for the first time that bioconjugation provides a newly developed and improved approach to produce an Sd1 glycoconjugate that can be characterized using state-of-the-art techniques.
T10 1669-1870 Sentence denotes In addition, this generic process together with the analytical methods is ideally suited for the production of additional Shigella serotypes, allowing the development of a multivalent Shigella vaccine.

GlycoBiology-PACDB

Id Subject Object Predicate Lexical cue
_T1 96-112 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC002,LEC056,LEC062,LEC069,LEC081,LEC111,LEC133,LEC171,LEC177,LEC187,LEC211,LEC242,LEC252,LEC258,LEC259,LEC260,LEC262,LEC369,LEC377,LEC422,LEC442,LEC448,LEC450,LEC451,LEC454,LEC472,LEC492,LEC620 denotes Escherichia coli
_T2 96-112 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC636 denotes Escherichia coli
_T3 96-112 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC157,LEC407 denotes Escherichia coli
_T4 96-112 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC295,LEC417 denotes Escherichia coli
_T5 96-112 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC754 denotes Escherichia coli
_T6 96-112 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC244,LEC256,LEC354 denotes Escherichia coli
_T7 96-112 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC243,LEC640 denotes Escherichia coli
_T8 96-112 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC487 denotes Escherichia coli
_T9 96-112 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC054,LEC058,LEC073,LEC082,LEC091,LEC103,LEC109,LEC110,LEC123,LEC158,LEC179,LEC198,LEC205,LEC222,LEC223,LEC224,LEC225,LEC232,LEC298,LEC357,LEC378,LEC383,LEC388,LEC389,LEC397,LEC401,LEC410,LEC452 denotes Escherichia coli
_T10 753-769 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC295,LEC417 denotes Escherichia coli
_T11 753-769 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC243,LEC640 denotes Escherichia coli
_T12 753-769 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC487 denotes Escherichia coli
_T13 753-769 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC754 denotes Escherichia coli
_T14 753-769 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC244,LEC256,LEC354 denotes Escherichia coli
_T15 753-769 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC157,LEC407 denotes Escherichia coli
_T16 753-769 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC636 denotes Escherichia coli
_T17 753-769 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC002,LEC056,LEC062,LEC069,LEC081,LEC111,LEC133,LEC171,LEC177,LEC187,LEC211,LEC242,LEC252,LEC258,LEC259,LEC260,LEC262,LEC369,LEC377,LEC422,LEC442,LEC448,LEC450,LEC451,LEC454,LEC472,LEC492,LEC620 denotes Escherichia coli
_T18 753-769 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC054,LEC058,LEC073,LEC082,LEC091,LEC103,LEC109,LEC110,LEC123,LEC158,LEC179,LEC198,LEC205,LEC222,LEC223,LEC224,LEC225,LEC232,LEC298,LEC357,LEC378,LEC383,LEC388,LEC389,LEC397,LEC401,LEC410,LEC452 denotes Escherichia coli
_T19 863-883 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC240,LEC496,LEC580 denotes Shigella dysenteriae
_T20 863-890 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC542 denotes Shigella dysenteriae type 1
_T21 863-890 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC607 denotes Shigella dysenteriae type 1
_T22 863-890 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC071 denotes Shigella dysenteriae type 1
_T23 1251-1273 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC466 denotes Pseudomonas aeruginosa
_T24 1251-1273 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC703 denotes Pseudomonas aeruginosa
_T25 1251-1273 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC721 denotes Pseudomonas aeruginosa
_T26 1251-1273 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC325 denotes Pseudomonas aeruginosa
_T27 1251-1273 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC709 denotes Pseudomonas aeruginosa
_T28 1251-1273 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC001,LEC005,LEC007,LEC029,LEC030,LEC032,LEC135,LEC191,LEC229,LEC231,LEC368,LEC447,LEC617,LEC676,PGG008 denotes Pseudomonas aeruginosa
_T29 1251-1273 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC584 denotes Pseudomonas aeruginosa

ICD10

Id Subject Object Predicate Lexical cue
T1 114-125 http://purl.bioontology.org/ontology/ICD10/A03 denotes Shigellosis
T2 511-522 http://purl.bioontology.org/ontology/ICD10/A03 denotes Shigellosis
T3 114-125 http://purl.bioontology.org/ontology/ICD10/A03.9 denotes Shigellosis
T4 511-522 http://purl.bioontology.org/ontology/ICD10/A03.9 denotes Shigellosis
T5 413-433 http://purl.bioontology.org/ontology/ICD10/A49.9 denotes bacterial infections

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 204-215 FMAID:188924 denotes substantial
_T2 253-267 FMAID:82782 denotes Glycoconjugate
_T3 253-267 FMAID:196776 denotes Glycoconjugate
_T4 301-308 FMAID:146300 denotes surface
_T5 301-308 FMAID:50594 denotes surface
_T6 309-324 FMAID:82746 denotes polysaccharides
_T7 309-324 FMAID:82785 denotes polysaccharides
_T8 309-324 FMAID:196735 denotes polysaccharides
_T9 309-324 FMAID:196779 denotes polysaccharides
_T10 347-355 FMAID:67257 denotes proteins
_T11 347-355 FMAID:165447 denotes proteins
_T12 585-592 FMAID:146300 denotes surface
_T13 585-592 FMAID:50594 denotes surface
_T14 593-607 FMAID:196735 denotes polysaccharide
_T15 593-607 FMAID:196779 denotes polysaccharide
_T16 593-607 FMAID:82746 denotes polysaccharide
_T17 593-607 FMAID:82785 denotes polysaccharide
_T18 706-713 FMAID:165447 denotes protein
_T19 706-713 FMAID:67257 denotes protein
_T20 791-804 FMAID:188924 denotes substantially
_T21 834-849 FMAID:82782 denotes glycoconjugates
_T22 834-849 FMAID:196776 denotes glycoconjugates
_T23 899-913 FMAID:82785 denotes polysaccharide
_T24 899-913 FMAID:82746 denotes polysaccharide
_T25 899-913 FMAID:196735 denotes polysaccharide
_T26 899-913 FMAID:196779 denotes polysaccharide
_T27 986-991 FMAID:67264 denotes lipid
_T28 986-991 FMAID:165448 denotes lipid
_T29 1052-1066 FMAID:196735 denotes polysaccharide
_T30 1052-1066 FMAID:196779 denotes polysaccharide
_T31 1052-1066 FMAID:82785 denotes polysaccharide
_T32 1052-1066 FMAID:82746 denotes polysaccharide
_T33 1107-1117 FMAID:82750 denotes asparagine
_T34 1107-1117 FMAID:196739 denotes asparagine
_T35 1142-1149 FMAID:165447 denotes protein
_T36 1142-1149 FMAID:67257 denotes protein
_T37 1221-1228 FMAID:67257 denotes protein
_T38 1221-1228 FMAID:165447 denotes protein
_T39 1323-1337 FMAID:196776 denotes glycoconjugate
_T40 1323-1337 FMAID:82782 denotes glycoconjugate
_T41 1593-1607 FMAID:82782 denotes glycoconjugate
_T42 1593-1607 FMAID:196776 denotes glycoconjugate

uniprot-human

Id Subject Object Predicate Lexical cue
T1 1241-1244 http://www.uniprot.org/uniprot/P01033 denotes EPA
T2 1343-1346 http://www.uniprot.org/uniprot/P01033 denotes EPA

uniprot-mouse

Id Subject Object Predicate Lexical cue
T1 1241-1244 http://www.uniprot.org/uniprot/P12032 denotes EPA
T2 1343-1346 http://www.uniprot.org/uniprot/P12032 denotes EPA
T3 1653-1656 http://www.uniprot.org/uniprot/Q8K4J0 denotes art
T4 1653-1656 http://www.uniprot.org/uniprot/P56473 denotes art

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 96-107 http://purl.bioontology.org/ontology/NCBITAXON/561 denotes Escherichia
T2 243-251 http://purl.bioontology.org/ontology/NCBITAXON/525814 denotes children
T3 753-764 http://purl.bioontology.org/ontology/NCBITAXON/561 denotes Escherichia
T4 863-883 http://purl.bioontology.org/ontology/NCBITAXON/622 denotes Shigella dysenteriae
T5 1153-1155 http://purl.bioontology.org/ontology/NCBITAXON/13893 denotes co
T6 1251-1262 http://purl.bioontology.org/ontology/NCBITAXON/651740 denotes Pseudomonas
T7 1251-1262 http://purl.bioontology.org/ontology/NCBITAXON/135621 denotes Pseudomonas
T8 1251-1273 http://purl.bioontology.org/ontology/NCBITAXON/287 denotes Pseudomonas aeruginosa

GO-BP

Id Subject Object Predicate Lexical cue
T1 48-57 http://purl.obolibrary.org/obo/GO_0000746 denotes conjugate
T2 325-335 http://purl.obolibrary.org/obo/GO_0000746 denotes conjugated
T3 681-692 http://purl.obolibrary.org/obo/GO_0000746 denotes conjugation
T4 172-182 http://purl.obolibrary.org/obo/GO_0032502 denotes developing
T5 453-464 http://purl.obolibrary.org/obo/GO_0032502 denotes development
T6 1543-1552 http://purl.obolibrary.org/obo/GO_0032502 denotes developed
T7 666-692 http://purl.obolibrary.org/obo/GO_0031136 denotes activation and conjugation
T8 770-783 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T9 969-977 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosyl

EDAM-topics

Id Subject Object Predicate Lexical cue
T1 161-168 http://edamontology.org/topic_0634 denotes disease
T2 347-355 http://edamontology.org/topic_0078 denotes proteins
T3 706-713 http://edamontology.org/topic_0078 denotes protein
T4 946-954 http://edamontology.org/topic_0196 denotes assembly
T5 986-991 http://edamontology.org/topic_0153 denotes lipid
T6 1029-1046 http://edamontology.org/topic_0134 denotes mass spectrometry
T7 1029-1046 http://edamontology.org/topic_3520 denotes mass spectrometry
T8 1142-1149 http://edamontology.org/topic_0078 denotes protein
T9 1142-1166 http://edamontology.org/topic_0121 denotes protein by co-expression
T10 1142-1166 http://edamontology.org/topic_0108 denotes protein by co-expression
T11 1221-1228 http://edamontology.org/topic_0078 denotes protein
T12 1430-1433 http://edamontology.org/topic_0593 denotes NMR
T13 1438-1455 http://edamontology.org/topic_3520 denotes mass spectrometry
T14 1438-1455 http://edamontology.org/topic_0134 denotes mass spectrometry

EDAM-DFO

Id Subject Object Predicate Lexical cue
T1 347-355 http://edamontology.org/format_1208 denotes proteins
T2 347-355 http://edamontology.org/data_1467 denotes proteins
T3 570-577 http://edamontology.org/operation_2409 denotes process
T4 570-577 http://edamontology.org/operation_0004 denotes process
T5 706-713 http://edamontology.org/data_1467 denotes protein
T6 706-713 http://edamontology.org/format_1208 denotes protein
T7 946-954 http://edamontology.org/operation_3433 denotes assembly
T8 995-1007 http://edamontology.org/operation_2246 denotes demonstrated
T9 1016-1024 http://edamontology.org/operation_2945 denotes analysis
T10 1029-1046 http://edamontology.org/data_2536 denotes mass spectrometry
T11 1029-1046 http://edamontology.org/data_3147 denotes mass spectrometry
T12 1118-1126 http://edamontology.org/data_1756 denotes residues
T13 1142-1149 http://edamontology.org/data_1467 denotes protein
T14 1142-1149 http://edamontology.org/format_1208 denotes protein
T15 1221-1228 http://edamontology.org/data_1467 denotes protein
T16 1221-1228 http://edamontology.org/format_1208 denotes protein
T17 1438-1455 http://edamontology.org/data_3147 denotes mass spectrometry
T18 1438-1455 http://edamontology.org/data_2536 denotes mass spectrometry
T19 1474-1480 http://edamontology.org/data_2048 denotes report
T20 1687-1694 http://edamontology.org/operation_3429 denotes generic
T21 1695-1702 http://edamontology.org/operation_2409 denotes process
T22 1695-1702 http://edamontology.org/operation_0004 denotes process

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 114-125 Disease denotes Shigellosis http://purl.obolibrary.org/obo/MONDO_0019345
T2 151-168 Disease denotes diarrheal disease http://purl.obolibrary.org/obo/MONDO_0001673
T3 413-433 Disease denotes bacterial infections http://purl.obolibrary.org/obo/MONDO_0005113
T4 511-522 Disease denotes Shigellosis http://purl.obolibrary.org/obo/MONDO_0019345
T5 892-895 Disease denotes Sd1 http://purl.obolibrary.org/obo/MONDO_0008512
T6 1339-1342 Disease denotes Sd1 http://purl.obolibrary.org/obo/MONDO_0008512
T7 1589-1592 Disease denotes Sd1 http://purl.obolibrary.org/obo/MONDO_0008512

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 39-47 OrganismTaxon denotes Shigella 620
T2 96-112 OrganismTaxon denotes Escherichia coli 562
T3 753-769 OrganismTaxon denotes Escherichia coli 562
T4 863-883 OrganismTaxon denotes Shigella dysenteriae 622
T5 961-968 OrganismTaxon denotes E. coli 562
T6 1251-1273 OrganismTaxon denotes Pseudomonas aeruginosa 287
T7 1314-1322 OrganismTaxon denotes Shigella 620
T8 1791-1799 OrganismTaxon denotes Shigella 620
T9 1853-1861 OrganismTaxon denotes Shigella 620