> top > docs > PubMed:26272610 > annotations

PubMed:26272610 JSONTXT

Annnotations TAB JSON ListView MergeView

sentences

Id Subject Object Predicate Lexical cue
T1 0-149 Sentence denotes Catalysis of GTP hydrolysis by small GTPases at atomic detail by integration of X-ray crystallography, experimental, and theoretical IR spectroscopy.
T2 150-196 Sentence denotes Small GTPases regulate key processes in cells.
T3 197-278 Sentence denotes Malfunction of their GTPase reaction by mutations is involved in severe diseases.
T4 279-362 Sentence denotes Here, we compare the GTPase reaction of the slower hydrolyzing GTPase Ran with Ras.
T5 363-590 Sentence denotes By combination of time-resolved FTIR difference spectroscopy and QM/MM simulations we elucidate that the Mg(2+) coordination by the phosphate groups, which varies largely among the x-ray structures, is the same for Ran and Ras.
T6 591-757 Sentence denotes A new x-ray structure of a Ran·RanBD1 complex with improved resolution confirmed this finding and revealed a general problem with the refinement of Mg(2+) in GTPases.
T7 758-844 Sentence denotes The Mg(2+) coordination is not responsible for the much slower GTPase reaction of Ran.
T8 845-1041 Sentence denotes Instead, the location of the Tyr-39 side chain of Ran between the γ-phosphate and Gln-69 prevents the optimal positioning of the attacking water molecule by the Gln-69 relative to the γ-phosphate.
T9 1042-1100 Sentence denotes This is confirmed in the RanY39A·RanBD1 crystal structure.
T10 1101-1218 Sentence denotes The QM/MM simulations provide IR spectra of the catalytic center, which agree very nicely with the experimental ones.
T11 1219-1305 Sentence denotes The combination of both methods can correlate spectra with structure at atomic detail.
T12 1306-1480 Sentence denotes For example the FTIR difference spectra of RasA18T and RanT25A mutants show that spectral differences are mainly due to the hydrogen bond of Thr-25 to the α-phosphate in Ran.
T13 1481-1712 Sentence denotes By integration of x-ray structure analysis, experimental, and theoretical IR spectroscopy the catalytic center of the x-ray structural models are further refined to sub-Å resolution, allowing an improved understanding of catalysis.