PubMed:26269597
Annnotations
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-118 | Sentence | denotes | ERK2-Pyruvate Kinase Axis Permits Phorbol 12-Myristate 13-Acetate-induced Megakaryocyte Differentiation in K562 Cells. |
| T2 | 119-196 | Sentence | denotes | Metabolic changes that contribute to differentiation are not well understood. |
| T3 | 197-332 | Sentence | denotes | Overwhelming evidence shows the critical role of glycolytic enzyme pyruvate kinase (PK) in directing metabolism of proliferating cells. |
| T4 | 333-401 | Sentence | denotes | However, its role in metabolism of differentiating cells is unclear. |
| T5 | 402-542 | Sentence | denotes | Here we studied the role of PK in phorbol 12-myristate 13-acetate (PMA)-induced megakaryocytic differentiation in human leukemia K562 cells. |
| T6 | 543-743 | Sentence | denotes | We observed that PMA treatment decreased cancer-type anabolic metabolism but increased ATP production, along with up-regulated expression of two PK isoforms (PKM2 and PKR) in an ERK2-dependent manner. |
| T7 | 744-950 | Sentence | denotes | Interestingly, silencing of PK (PKM2 and PKR) inhibited PMA-induced megakaryocytic differentiation, as revealed by decreased expression of megakaryocytic differentiation marker CD61 and cell cycle behavior. |
| T8 | 951-1071 | Sentence | denotes | Further, PMA-induced ATP production reduced greatly upon PK silencing, suggesting that PK is required for ATP synthesis. |
| T9 | 1072-1170 | Sentence | denotes | In addition to metabolic effects, PMA treatment also translocated PKM2, but not PKR, into nucleus. |
| T10 | 1171-1352 | Sentence | denotes | ERK1/2 knockdowns independently and together suggested the role of ERK2 in the up-regulation of both the isoforms of PK, proposing a role of ERK2-PK isoform axis in differentiation. |
| T11 | 1353-1507 | Sentence | denotes | Collectively, our findings unravel ERK2 guided PK-dependent metabolic changes during PMA induction, which are important in megakaryocytic differentiation. |
mondo_disease
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T1 | 469-472 | Disease | denotes | PMA | http://purl.obolibrary.org/obo/MONDO_0016692|http://purl.obolibrary.org/obo/MONDO_0018687 |
| T3 | 522-530 | Disease | denotes | leukemia | http://purl.obolibrary.org/obo/MONDO_0005059 |
| T4 | 560-563 | Disease | denotes | PMA | http://purl.obolibrary.org/obo/MONDO_0016692|http://purl.obolibrary.org/obo/MONDO_0018687 |
| T6 | 584-590 | Disease | denotes | cancer | http://purl.obolibrary.org/obo/MONDO_0004992 |
| T7 | 800-803 | Disease | denotes | PMA | http://purl.obolibrary.org/obo/MONDO_0016692|http://purl.obolibrary.org/obo/MONDO_0018687 |
| T9 | 960-963 | Disease | denotes | PMA | http://purl.obolibrary.org/obo/MONDO_0016692|http://purl.obolibrary.org/obo/MONDO_0018687 |
| T11 | 1106-1109 | Disease | denotes | PMA | http://purl.obolibrary.org/obo/MONDO_0016692|http://purl.obolibrary.org/obo/MONDO_0018687 |
| T13 | 1438-1441 | Disease | denotes | PMA | http://purl.obolibrary.org/obo/MONDO_0016692|http://purl.obolibrary.org/obo/MONDO_0018687 |
GlyCosmos15-HP
| Id | Subject | Object | Predicate | Lexical cue | hp_id |
|---|---|---|---|---|---|
| T1 | 522-530 | Phenotype | denotes | leukemia | HP:0001909 |
| T2 | 584-590 | Phenotype | denotes | cancer | HP:0002664 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 516-521 | OrganismTaxon | denotes | human | 9606 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 21-25 | Body_part | denotes | Axis | http://purl.obolibrary.org/obo/UBERON_0001093 |
| T2 | 74-87 | Body_part | denotes | Megakaryocyte | http://purl.obolibrary.org/obo/CL_0000556 |
| T3 | 482-496 | Body_part | denotes | megakaryocytic | http://purl.obolibrary.org/obo/CL_0000556 |
| T4 | 812-826 | Body_part | denotes | megakaryocytic | http://purl.obolibrary.org/obo/CL_0000556 |
| T5 | 883-897 | Body_part | denotes | megakaryocytic | http://purl.obolibrary.org/obo/CL_0000556 |
| T6 | 930-940 | Body_part | denotes | cell cycle | http://purl.obolibrary.org/obo/UBERON_2000098 |
| T7 | 1162-1169 | Body_part | denotes | nucleus | http://purl.obolibrary.org/obo/GO_0005634|http://purl.obolibrary.org/obo/UBERON_0000125 |
| T9 | 1328-1332 | Body_part | denotes | axis | http://purl.obolibrary.org/obo/UBERON_0001093 |
| T10 | 1476-1490 | Body_part | denotes | megakaryocytic | http://purl.obolibrary.org/obo/CL_0000556 |
Glycosmos15-CL
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 74-87 | Cell | denotes | Megakaryocyte | http://purl.obolibrary.org/obo/CL:0000556 |
| T2 | 482-496 | Cell | denotes | megakaryocytic | http://purl.obolibrary.org/obo/CL:0000556 |
| T3 | 812-826 | Cell | denotes | megakaryocytic | http://purl.obolibrary.org/obo/CL:0000556 |
| T4 | 883-897 | Cell | denotes | megakaryocytic | http://purl.obolibrary.org/obo/CL:0000556 |
| T5 | 1476-1490 | Cell | denotes | megakaryocytic | http://purl.obolibrary.org/obo/CL:0000556 |