| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-104 |
Sentence |
denotes |
Connexin Type and Fluorescent Protein Fusion Tag Determine Structural Stability of Gap Junction Plaques. |
| T2 |
105-247 |
Sentence |
denotes |
Gap junctions (GJs) are made up of plaques of laterally clustered intercellular channels and the membranes in which the channels are embedded. |
| T3 |
248-390 |
Sentence |
denotes |
Arrangement of channels within a plaque determines subcellular distribution of connexin binding partners and sites of intercellular signaling. |
| T4 |
391-583 |
Sentence |
denotes |
Here, we report the discovery that some connexin types form plaque structures with strikingly different degrees of fluidity in the arrangement of the GJ channel subcomponents of the GJ plaque. |
| T5 |
584-738 |
Sentence |
denotes |
We uncovered this property of GJs by applying fluorescence recovery after photobleaching to GJs formed from connexins fused with fluorescent protein tags. |
| T6 |
739-913 |
Sentence |
denotes |
We found that connexin 26 (Cx26) and Cx30 GJs readily diffuse within the plaque structures, whereas Cx43 GJs remain persistently immobile for more than 2 min after bleaching. |
| T7 |
914-1003 |
Sentence |
denotes |
The cytoplasmic C terminus of Cx43 was required for stability of Cx43 plaque arrangement. |
| T8 |
1004-1220 |
Sentence |
denotes |
We provide evidence that these qualitative differences in GJ arrangement stability reflect endogenous characteristics, with the caveat that the sizes of the GJs examined were necessarily large for these measurements. |
| T9 |
1221-1401 |
Sentence |
denotes |
We also uncovered an unrecognized effect of non-monomerized fluorescent protein on the dynamically arranged GJs and the organization of plaques composed of multiple connexin types. |
| T10 |
1402-1609 |
Sentence |
denotes |
Together, these findings redefine our understanding of the GJ plaque structure and should be considered in future studies using fluorescent protein tags to probe dynamics of highly ordered protein complexes. |
