PubMed:26254469
Annnotations
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-128 | Sentence | denotes | The Ribosome-Sec61 Translocon Complex Forms a Cytosolically Restricted Environment for Early Polytopic Membrane Protein Folding. |
| T2 | 129-355 | Sentence | denotes | Transmembrane topology of polytopic membrane proteins (PMPs) is established in the endoplasmic reticulum (ER) by the ribosome Sec61-translocon complex (RTC) through iterative cycles of translocation initiation and termination. |
| T3 | 356-578 | Sentence | denotes | It remains unknown, however, whether tertiary folding of transmembrane domains begins after the nascent polypeptide integrates into the lipid bilayer or within a proteinaceous environment proximal to translocon components. |
| T4 | 579-869 | Sentence | denotes | To address this question, we used cysteine scanning mutagenesis to monitor aqueous accessibility of stalled translation intermediates to determine when, during biogenesis, hydrophilic peptide loops of the aquaporin-4 (AQP4) water channel are delivered to cytosolic and lumenal compartments. |
| T5 | 870-1035 | Sentence | denotes | Results showed that following ribosome docking on the ER membrane, the nascent polypeptide was shielded from the cytosol as it emerged from the ribosome exit tunnel. |
| T6 | 1036-1223 | Sentence | denotes | Extracellular loops followed a well defined path through the ribosome, the ribosome translocon junction, the Sec61-translocon pore, and into the ER lumen coincident with chain elongation. |
| T7 | 1224-1451 | Sentence | denotes | In contrast, intracellular loops (ICLs) and C-terminalresidues exited the ribosome into a cytosolically shielded environment and remained inaccessible to both cytosolic and lumenal compartments until translation was terminated. |
| T8 | 1452-1583 | Sentence | denotes | Shielding of ICL1 and ICL2, but not the C terminus, became resistant to maneuvers that disrupt electrostatic ribosome interactions. |
| T9 | 1584-1747 | Sentence | denotes | Thus, the early folding landscape of polytopic proteins is shaped by a spatially restricted environment localized within the assembled ribosome translocon complex. |
Glycosmos6-MAT
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 544-552 | http://purl.obolibrary.org/obo/MAT_0000491 | denotes | proximal |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 4-12 | Body_part | denotes | Ribosome | http://purl.obolibrary.org/obo/GO_0005840 |
| T2 | 103-111 | Body_part | denotes | Membrane | http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158 |
| T5 | 129-142 | Body_part | denotes | Transmembrane | http://purl.obolibrary.org/obo/GO_0016020 |
| T6 | 165-173 | Body_part | denotes | membrane | http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158 |
| T9 | 224-233 | Body_part | denotes | reticulum | http://purl.obolibrary.org/obo/UBERON_0007361 |
| T10 | 246-254 | Body_part | denotes | ribosome | http://purl.obolibrary.org/obo/GO_0005840 |
| T11 | 413-426 | Body_part | denotes | transmembrane | http://purl.obolibrary.org/obo/GO_0016020 |
| T12 | 900-908 | Body_part | denotes | ribosome | http://purl.obolibrary.org/obo/GO_0005840 |
| T13 | 927-935 | Body_part | denotes | membrane | http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158 |
| T16 | 965-973 | Body_part | denotes | shielded | http://purl.obolibrary.org/obo/UBERON_0003062 |
| T17 | 983-990 | Body_part | denotes | cytosol | http://purl.obolibrary.org/obo/GO_0005829 |
| T18 | 1014-1022 | Body_part | denotes | ribosome | http://purl.obolibrary.org/obo/GO_0005840 |
| T19 | 1036-1049 | Body_part | denotes | Extracellular | http://purl.obolibrary.org/obo/GO_0005576 |
| T20 | 1097-1105 | Body_part | denotes | ribosome | http://purl.obolibrary.org/obo/GO_0005840 |
| T21 | 1111-1119 | Body_part | denotes | ribosome | http://purl.obolibrary.org/obo/GO_0005840 |
| T22 | 1131-1139 | Body_part | denotes | junction | http://purl.obolibrary.org/obo/UBERON_0007651 |
| T23 | 1162-1166 | Body_part | denotes | pore | http://purl.obolibrary.org/obo/UBERON_0008915 |
| T24 | 1184-1189 | Body_part | denotes | lumen | http://purl.obolibrary.org/obo/UBERON_0000464 |
| T25 | 1237-1250 | Body_part | denotes | intracellular | http://purl.obolibrary.org/obo/GO_0005622 |
| T26 | 1298-1306 | Body_part | denotes | ribosome | http://purl.obolibrary.org/obo/GO_0005840 |
| T27 | 1328-1336 | Body_part | denotes | shielded | http://purl.obolibrary.org/obo/UBERON_0003062 |
| T28 | 1561-1569 | Body_part | denotes | ribosome | http://purl.obolibrary.org/obo/GO_0005840 |
| T29 | 1719-1727 | Body_part | denotes | ribosome | http://purl.obolibrary.org/obo/GO_0005840 |
Anatomy-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 544-552 | Body_part | denotes | proximal | http://purl.obolibrary.org/obo/MAT_0000491 |