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PubMed:26254469 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-128 Sentence denotes The Ribosome-Sec61 Translocon Complex Forms a Cytosolically Restricted Environment for Early Polytopic Membrane Protein Folding.
T2 129-355 Sentence denotes Transmembrane topology of polytopic membrane proteins (PMPs) is established in the endoplasmic reticulum (ER) by the ribosome Sec61-translocon complex (RTC) through iterative cycles of translocation initiation and termination.
T3 356-578 Sentence denotes It remains unknown, however, whether tertiary folding of transmembrane domains begins after the nascent polypeptide integrates into the lipid bilayer or within a proteinaceous environment proximal to translocon components.
T4 579-869 Sentence denotes To address this question, we used cysteine scanning mutagenesis to monitor aqueous accessibility of stalled translation intermediates to determine when, during biogenesis, hydrophilic peptide loops of the aquaporin-4 (AQP4) water channel are delivered to cytosolic and lumenal compartments.
T5 870-1035 Sentence denotes Results showed that following ribosome docking on the ER membrane, the nascent polypeptide was shielded from the cytosol as it emerged from the ribosome exit tunnel.
T6 1036-1223 Sentence denotes Extracellular loops followed a well defined path through the ribosome, the ribosome translocon junction, the Sec61-translocon pore, and into the ER lumen coincident with chain elongation.
T7 1224-1451 Sentence denotes In contrast, intracellular loops (ICLs) and C-terminalresidues exited the ribosome into a cytosolically shielded environment and remained inaccessible to both cytosolic and lumenal compartments until translation was terminated.
T8 1452-1583 Sentence denotes Shielding of ICL1 and ICL2, but not the C terminus, became resistant to maneuvers that disrupt electrostatic ribosome interactions.
T9 1584-1747 Sentence denotes Thus, the early folding landscape of polytopic proteins is shaped by a spatially restricted environment localized within the assembled ribosome translocon complex.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 544-552 http://purl.obolibrary.org/obo/MAT_0000491 denotes proximal

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 4-12 Body_part denotes Ribosome http://purl.obolibrary.org/obo/GO_0005840
T2 103-111 Body_part denotes Membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T5 129-142 Body_part denotes Transmembrane http://purl.obolibrary.org/obo/GO_0016020
T6 165-173 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T9 224-233 Body_part denotes reticulum http://purl.obolibrary.org/obo/UBERON_0007361
T10 246-254 Body_part denotes ribosome http://purl.obolibrary.org/obo/GO_0005840
T11 413-426 Body_part denotes transmembrane http://purl.obolibrary.org/obo/GO_0016020
T12 900-908 Body_part denotes ribosome http://purl.obolibrary.org/obo/GO_0005840
T13 927-935 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T16 965-973 Body_part denotes shielded http://purl.obolibrary.org/obo/UBERON_0003062
T17 983-990 Body_part denotes cytosol http://purl.obolibrary.org/obo/GO_0005829
T18 1014-1022 Body_part denotes ribosome http://purl.obolibrary.org/obo/GO_0005840
T19 1036-1049 Body_part denotes Extracellular http://purl.obolibrary.org/obo/GO_0005576
T20 1097-1105 Body_part denotes ribosome http://purl.obolibrary.org/obo/GO_0005840
T21 1111-1119 Body_part denotes ribosome http://purl.obolibrary.org/obo/GO_0005840
T22 1131-1139 Body_part denotes junction http://purl.obolibrary.org/obo/UBERON_0007651
T23 1162-1166 Body_part denotes pore http://purl.obolibrary.org/obo/UBERON_0008915
T24 1184-1189 Body_part denotes lumen http://purl.obolibrary.org/obo/UBERON_0000464
T25 1237-1250 Body_part denotes intracellular http://purl.obolibrary.org/obo/GO_0005622
T26 1298-1306 Body_part denotes ribosome http://purl.obolibrary.org/obo/GO_0005840
T27 1328-1336 Body_part denotes shielded http://purl.obolibrary.org/obo/UBERON_0003062
T28 1561-1569 Body_part denotes ribosome http://purl.obolibrary.org/obo/GO_0005840
T29 1719-1727 Body_part denotes ribosome http://purl.obolibrary.org/obo/GO_0005840

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 544-552 Body_part denotes proximal http://purl.obolibrary.org/obo/MAT_0000491