PubMed:26254468
Annnotations
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-153 | Sentence | denotes | Cumulin, an Oocyte-secreted Heterodimer of the Transforming Growth Factor-β Family, Is a Potent Activator of Granulosa Cells and Improves Oocyte Quality. |
| T2 | 154-423 | Sentence | denotes | Growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) are oocyte-specific growth factors with central roles in mammalian reproduction, regulating species-specific fecundity, ovarian follicular somatic cell differentiation, and oocyte quality. |
| T3 | 424-524 | Sentence | denotes | In the human, GDF9 is produced in a latent form, the mechanism of activation being an open question. |
| T4 | 525-704 | Sentence | denotes | Here, we produced a range of recombinant GDF9 and BMP15 variants, examined their in silico and physical interactions and their effects on ovarian granulosa cells (GC) and oocytes. |
| T5 | 705-856 | Sentence | denotes | We found that the potent synergistic actions of GDF9 and BMP15 on GC can be attributed to the formation of a heterodimer, which we have termed cumulin. |
| T6 | 857-1011 | Sentence | denotes | Structural modeling of cumulin revealed a dimerization interface identical to homodimeric GDF9 and BMP15, indicating likely formation of a stable complex. |
| T7 | 1012-1158 | Sentence | denotes | This was confirmed by generation of recombinant heterodimeric complexes of pro/mature domains (pro-cumulin) and covalent mature domains (cumulin). |
| T8 | 1159-1401 | Sentence | denotes | Both pro-cumulin and cumulin exhibited highly potent bioactivity on GC, activating both SMAD2/3 and SMAD1/5/8 signaling pathways and promoting proliferation and expression of a set of genes associated with oocyte-regulated GC differentiation. |
| T9 | 1402-1491 | Sentence | denotes | Cumulin was more potent than pro-cumulin, pro-GDF9, pro-BMP15, or the two combined on GC. |
| T10 | 1492-1678 | Sentence | denotes | However, on cumulus-oocyte complexes, pro-cumulin was more effective than all other growth factors at notably improving oocyte quality as assessed by subsequent day 7 embryo development. |
| T11 | 1679-1805 | Sentence | denotes | Our results support a model of activation for human GDF9 dependent on cumulin formation through heterodimerization with BMP15. |
| T12 | 1806-1902 | Sentence | denotes | Oocyte-secreted cumulin is likely to be a central regulator of fertility in mono-ovular mammals. |
Glycosmos6-MAT
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 197-201 | http://purl.obolibrary.org/obo/MAT_0000299 | denotes | bone |
| T2 | 1659-1665 | http://purl.obolibrary.org/obo/MAT_0000226 | denotes | embryo |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 431-436 | OrganismTaxon | denotes | human | 9606 |
| T2 | 1725-1730 | OrganismTaxon | denotes | human | 9606 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 12-18 | Body_part | denotes | Oocyte | http://purl.obolibrary.org/obo/CL_0000023 |
| T2 | 109-124 | Body_part | denotes | Granulosa Cells | http://purl.obolibrary.org/obo/CL_0000501 |
| T3 | 138-144 | Body_part | denotes | Oocyte | http://purl.obolibrary.org/obo/CL_0000023 |
| T4 | 197-201 | Body_part | denotes | bone | http://purl.obolibrary.org/obo/UBERON_0001474|http://purl.obolibrary.org/obo/UBERON_0002481 |
| T6 | 239-245 | Body_part | denotes | oocyte | http://purl.obolibrary.org/obo/CL_0000023 |
| T7 | 408-414 | Body_part | denotes | oocyte | http://purl.obolibrary.org/obo/CL_0000023 |
| T8 | 671-686 | Body_part | denotes | granulosa cells | http://purl.obolibrary.org/obo/CL_0000501 |
| T9 | 688-690 | Body_part | denotes | GC | http://purl.obolibrary.org/obo/CL_0000501 |
| T10 | 771-773 | Body_part | denotes | GC | http://purl.obolibrary.org/obo/CL_0000501 |
| T11 | 1227-1229 | Body_part | denotes | GC | http://purl.obolibrary.org/obo/CL_0000501 |
| T12 | 1365-1371 | Body_part | denotes | oocyte | http://purl.obolibrary.org/obo/CL_0000023 |
| T13 | 1382-1384 | Body_part | denotes | GC | http://purl.obolibrary.org/obo/CL_0000501 |
| T14 | 1488-1490 | Body_part | denotes | GC | http://purl.obolibrary.org/obo/CL_0000501 |
| T15 | 1512-1518 | Body_part | denotes | oocyte | http://purl.obolibrary.org/obo/CL_0000023 |
| T16 | 1612-1618 | Body_part | denotes | oocyte | http://purl.obolibrary.org/obo/CL_0000023 |
| T17 | 1659-1665 | Body_part | denotes | embryo | http://purl.obolibrary.org/obo/UBERON_0000922 |
| T18 | 1806-1812 | Body_part | denotes | Oocyte | http://purl.obolibrary.org/obo/CL_0000023 |
Anatomy-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 197-201 | Body_part | denotes | bone | http://purl.obolibrary.org/obo/MAT_0000299 |
| T2 | 1659-1665 | Body_part | denotes | embryo | http://purl.obolibrary.org/obo/MAT_0000226 |
CL-cell
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 12-18 | Cell | denotes | Oocyte | http://purl.obolibrary.org/obo/CL:0000023 |
| T2 | 109-124 | Cell | denotes | Granulosa Cells | http://purl.obolibrary.org/obo/CL:0000501 |
| T3 | 138-144 | Cell | denotes | Oocyte | http://purl.obolibrary.org/obo/CL:0000023 |
| T4 | 239-245 | Cell | denotes | oocyte | http://purl.obolibrary.org/obo/CL:0000023 |
| T5 | 408-414 | Cell | denotes | oocyte | http://purl.obolibrary.org/obo/CL:0000023 |
| T6 | 671-686 | Cell | denotes | granulosa cells | http://purl.obolibrary.org/obo/CL:0000501 |
| T7 | 688-690 | Cell | denotes | GC | http://purl.obolibrary.org/obo/CL:0000501 |
| T8 | 696-703 | Cell | denotes | oocytes | http://purl.obolibrary.org/obo/CL:0000023 |
| T9 | 771-773 | Cell | denotes | GC | http://purl.obolibrary.org/obo/CL:0000501 |
| T10 | 1227-1229 | Cell | denotes | GC | http://purl.obolibrary.org/obo/CL:0000501 |
| T11 | 1365-1371 | Cell | denotes | oocyte | http://purl.obolibrary.org/obo/CL:0000023 |
| T12 | 1382-1384 | Cell | denotes | GC | http://purl.obolibrary.org/obo/CL:0000501 |
| T13 | 1488-1490 | Cell | denotes | GC | http://purl.obolibrary.org/obo/CL:0000501 |
| T14 | 1512-1518 | Cell | denotes | oocyte | http://purl.obolibrary.org/obo/CL:0000023 |
| T15 | 1612-1618 | Cell | denotes | oocyte | http://purl.obolibrary.org/obo/CL:0000023 |
| T16 | 1806-1812 | Cell | denotes | Oocyte | http://purl.obolibrary.org/obo/CL:0000023 |