PubMed:26253171 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/26253171","sourcedb":"PubMed","sourceid":"26253171","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/26253171","text":"Interactions of Yeast Dynein with Dynein Light Chain and Dynactin: GENERAL IMPLICATIONS FOR INTRINSICALLY DISORDERED DUPLEX SCAFFOLDS IN MULTIPROTEIN ASSEMBLIES.\nIntrinsically disordered protein (IDP) duplexes composed of two IDP chains cross-linked by bivalent partner proteins form scaffolds for assembly of multiprotein complexes. The N-terminal domain of dynein intermediate chain (N-IC) is one such IDP that forms a bivalent scaffold with multiple dynein light chains including LC8, a hub protein that promotes duplex formation of diverse IDP partners. N-IC also binds a subunit of the dynein regulator, dynactin. Here we characterize interactions of a yeast ortholog of N-IC (N-Pac11) with yeast LC8 (Dyn2) or with the intermediate chain-binding subunit of yeast dynactin (Nip100). Residue level changes in Pac11 structure are monitored by NMR spectroscopy, and binding energetics are monitored by isothermal titration calorimetry (ITC). N-Pac11 is monomeric and primarily disordered except for a single α-helix (SAH) at the N terminus and a short nascent helix, LH, flanked by the two Dyn2 recognition motifs. Upon binding Dyn2, the only Pac11 residues making direct protein-protein interactions are in and immediately flanking the recognition motifs. Dyn2 binding also orders LH residues of Pac11. Upon binding Nip100, only Pac11 SAH residues make direct protein-protein interactions, but LH residues at a distant sequence position and L1 residues in an adjacent linker are also ordered. The long distance, ligand-dependent ordering of residues reveals new elements of dynamic structure within IDP linker regions.","tracks":[{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":161},"obj":"Sentence"},{"id":"T2","span":{"begin":162,"end":333},"obj":"Sentence"},{"id":"T3","span":{"begin":334,"end":557},"obj":"Sentence"},{"id":"T4","span":{"begin":558,"end":618},"obj":"Sentence"},{"id":"T5","span":{"begin":619,"end":787},"obj":"Sentence"},{"id":"T6","span":{"begin":788,"end":943},"obj":"Sentence"},{"id":"T7","span":{"begin":944,"end":1116},"obj":"Sentence"},{"id":"T8","span":{"begin":1117,"end":1258},"obj":"Sentence"},{"id":"T9","span":{"begin":1259,"end":1305},"obj":"Sentence"},{"id":"T10","span":{"begin":1306,"end":1495},"obj":"Sentence"},{"id":"T11","span":{"begin":1496,"end":1621},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"},{"subj":"T11","pred":"source","obj":"sentences"}]},{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":1532,"end":1540},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:5200049"},{"subj":"T1","pred":"source","obj":"HP-phenotype"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":1012,"end":1017},"obj":"Body_part"},{"id":"T2","span":{"begin":1062,"end":1067},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0002488"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0002488"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T2","pred":"source","obj":"Anatomy-UBERON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#d893ec","default":true},{"id":"HP-phenotype","color":"#93ecbd"},{"id":"Anatomy-UBERON","color":"#eca393"}]}]}}