PubMed:26036195 JSONTXT

{"project":"Glycan-Motif","denotations":[{"id":"T1","span":{"begin":69,"end":80},"obj":"https://glytoucan.org/Structures/Glycans/G43702JT"},{"id":"T2","span":{"begin":92,"end":103},"obj":"https://glytoucan.org/Structures/Glycans/G43702JT"},{"id":"T3","span":{"begin":226,"end":250},"obj":"https://glytoucan.org/Structures/Glycans/G61418DU"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":69,"end":80},"obj":"Glycan_Motif"},{"id":"T2","span":{"begin":92,"end":103},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":226,"end":250},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT"},{"id":"A2","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G61418DU"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":91},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":92,"end":301},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":302,"end":364},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":365,"end":515},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":516,"end":599},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":600,"end":761},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":762,"end":807},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":808,"end":922},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":923,"end":1034},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1035,"end":1149},"obj":"Sentence"},{"id":"TextSentencer_T11","span":{"begin":1150,"end":1237},"obj":"Sentence"},{"id":"TextSentencer_T12","span":{"begin":1238,"end":1333},"obj":"Sentence"},{"id":"TextSentencer_T13","span":{"begin":1334,"end":1451},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":91},"obj":"Sentence"},{"id":"T2","span":{"begin":92,"end":301},"obj":"Sentence"},{"id":"T3","span":{"begin":302,"end":364},"obj":"Sentence"},{"id":"T4","span":{"begin":365,"end":515},"obj":"Sentence"},{"id":"T5","span":{"begin":516,"end":599},"obj":"Sentence"},{"id":"T6","span":{"begin":600,"end":761},"obj":"Sentence"},{"id":"T7","span":{"begin":762,"end":807},"obj":"Sentence"},{"id":"T8","span":{"begin":808,"end":922},"obj":"Sentence"},{"id":"T9","span":{"begin":923,"end":1034},"obj":"Sentence"},{"id":"T10","span":{"begin":1035,"end":1149},"obj":"Sentence"},{"id":"T11","span":{"begin":1150,"end":1237},"obj":"Sentence"},{"id":"T12","span":{"begin":1238,"end":1333},"obj":"Sentence"},{"id":"T13","span":{"begin":1334,"end":1451},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":91},"obj":"Sentence"},{"id":"T2","span":{"begin":92,"end":301},"obj":"Sentence"},{"id":"T3","span":{"begin":302,"end":364},"obj":"Sentence"},{"id":"T4","span":{"begin":365,"end":515},"obj":"Sentence"},{"id":"T5","span":{"begin":516,"end":599},"obj":"Sentence"},{"id":"T6","span":{"begin":600,"end":761},"obj":"Sentence"},{"id":"T7","span":{"begin":762,"end":807},"obj":"Sentence"},{"id":"T8","span":{"begin":808,"end":922},"obj":"Sentence"},{"id":"T9","span":{"begin":923,"end":1034},"obj":"Sentence"},{"id":"T10","span":{"begin":1035,"end":1149},"obj":"Sentence"},{"id":"T11","span":{"begin":1150,"end":1237},"obj":"Sentence"},{"id":"T12","span":{"begin":1238,"end":1333},"obj":"Sentence"},{"id":"T13","span":{"begin":1334,"end":1451},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":69,"end":80},"obj":"https://glytoucan.org/Structures/Glycans/G43702JT"},{"id":"T2","span":{"begin":92,"end":103},"obj":"https://glytoucan.org/Structures/Glycans/G43702JT"},{"id":"T3","span":{"begin":226,"end":250},"obj":"https://glytoucan.org/Structures/Glycans/G61418DU"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"Glycosmos6-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":69,"end":80},"obj":"http://www.glycoepitope.jp/epitopes/EP0081"},{"id":"T2","span":{"begin":92,"end":103},"obj":"http://www.glycoepitope.jp/epitopes/EP0081"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"GlycoBiology-FMA","denotations":[{"id":"_T1","span":{"begin":45,"end":55},"obj":"FMAID:167180"},{"id":"_T2","span":{"begin":69,"end":88},"obj":"FMAID:196838"},{"id":"_T3","span":{"begin":69,"end":88},"obj":"FMAID:82837"},{"id":"_T4","span":{"begin":92,"end":111},"obj":"FMAID:196838"},{"id":"_T5","span":{"begin":92,"end":111},"obj":"FMAID:82837"},{"id":"_T6","span":{"begin":125,"end":139},"obj":"FMAID:196779"},{"id":"_T7","span":{"begin":125,"end":139},"obj":"FMAID:196735"},{"id":"_T8","span":{"begin":125,"end":139},"obj":"FMAID:82785"},{"id":"_T9","span":{"begin":125,"end":139},"obj":"FMAID:82746"},{"id":"_T10","span":{"begin":158,"end":170},"obj":"FMAID:82744"},{"id":"_T11","span":{"begin":158,"end":170},"obj":"FMAID:196733"},{"id":"_T12","span":{"begin":182,"end":197},"obj":"FMAID:196793"},{"id":"_T13","span":{"begin":182,"end":197},"obj":"FMAID:82798"},{"id":"_T14","span":{"begin":237,"end":250},"obj":"FMAID:82792"},{"id":"_T15","span":{"begin":237,"end":250},"obj":"FMAID:196787"},{"id":"_T16","span":{"begin":410,"end":420},"obj":"FMAID:167180"},{"id":"_T17","span":{"begin":522,"end":532},"obj":"FMAID:167180"},{"id":"_T18","span":{"begin":577,"end":585},"obj":"FMAID:67104"},{"id":"_T19","span":{"begin":577,"end":585},"obj":"FMAID:165189"},{"id":"_T20","span":{"begin":577,"end":593},"obj":"FMAID:165192"},{"id":"_T21","span":{"begin":637,"end":647},"obj":"FMAID:167180"},{"id":"_T22","span":{"begin":777,"end":787},"obj":"FMAID:167180"},{"id":"_T23","span":{"begin":812,"end":822},"obj":"FMAID:167180"},{"id":"_T24","span":{"begin":907,"end":921},"obj":"FMAID:196735"},{"id":"_T25","span":{"begin":907,"end":921},"obj":"FMAID:82746"},{"id":"_T26","span":{"begin":907,"end":921},"obj":"FMAID:196779"},{"id":"_T27","span":{"begin":907,"end":921},"obj":"FMAID:82785"},{"id":"_T28","span":{"begin":949,"end":959},"obj":"FMAID:167180"},{"id":"_T29","span":{"begin":981,"end":988},"obj":"FMAID:146300"},{"id":"_T30","span":{"begin":981,"end":988},"obj":"FMAID:50594"},{"id":"_T31","span":{"begin":1185,"end":1195},"obj":"FMAID:167180"},{"id":"_T32","span":{"begin":1281,"end":1291},"obj":"FMAID:167180"},{"id":"_T33","span":{"begin":1338,"end":1348},"obj":"FMAID:167180"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":595,"end":597},"obj":"http://www.uniprot.org/uniprot/P49366"},{"id":"T2","span":{"begin":681,"end":683},"obj":"http://www.uniprot.org/uniprot/P49366"},{"id":"T3","span":{"begin":688,"end":690},"obj":"http://www.uniprot.org/uniprot/P49366"},{"id":"T4","span":{"begin":804,"end":806},"obj":"http://www.uniprot.org/uniprot/P49366"},{"id":"T5","span":{"begin":664,"end":667},"obj":"http://www.uniprot.org/uniprot/Q13216"},{"id":"T6","span":{"begin":672,"end":675},"obj":"http://www.uniprot.org/uniprot/Q13216"},{"id":"T7","span":{"begin":1030,"end":1033},"obj":"http://www.uniprot.org/uniprot/Q13216"},{"id":"T8","span":{"begin":1145,"end":1148},"obj":"http://www.uniprot.org/uniprot/Q13216"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":34,"end":44},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/608684"},{"id":"T2","span":{"begin":321,"end":325},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/9973"},{"id":"T3","span":{"begin":399,"end":409},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/608684"},{"id":"T4","span":{"begin":422,"end":428},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/62951"},{"id":"T5","span":{"begin":1270,"end":1280},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/608684"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":81,"end":88},"obj":"http://purl.obolibrary.org/obo/GO_0051923"},{"id":"T2","span":{"begin":104,"end":111},"obj":"http://purl.obolibrary.org/obo/GO_0051923"},{"id":"T3","span":{"begin":586,"end":593},"obj":"http://purl.obolibrary.org/obo/GO_0051923"},{"id":"T4","span":{"begin":655,"end":663},"obj":"http://purl.obolibrary.org/obo/GO_0051923"},{"id":"T5","span":{"begin":199,"end":203},"obj":"http://purl.obolibrary.org/obo/GO_0043879"},{"id":"T6","span":{"begin":875,"end":879},"obj":"http://purl.obolibrary.org/obo/GO_0043879"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"GO-MF","denotations":[{"id":"T1","span":{"begin":45,"end":55},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T2","span":{"begin":410,"end":420},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T3","span":{"begin":522,"end":532},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T4","span":{"begin":637,"end":647},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T5","span":{"begin":777,"end":787},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T6","span":{"begin":812,"end":822},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T7","span":{"begin":949,"end":959},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T8","span":{"begin":1185,"end":1195},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T9","span":{"begin":1281,"end":1291},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T10","span":{"begin":1338,"end":1348},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T11","span":{"begin":907,"end":930},"obj":"http://purl.obolibrary.org/obo/GO_0030247"},{"id":"T12","span":{"begin":923,"end":930},"obj":"http://purl.obolibrary.org/obo/GO_0070026"},{"id":"T13","span":{"begin":1039,"end":1046},"obj":"http://purl.obolibrary.org/obo/GO_0070026"},{"id":"T14","span":{"begin":923,"end":930},"obj":"http://purl.obolibrary.org/obo/GO_0003680"},{"id":"T15","span":{"begin":1039,"end":1046},"obj":"http://purl.obolibrary.org/obo/GO_0003680"},{"id":"T16","span":{"begin":923,"end":930},"obj":"http://purl.obolibrary.org/obo/GO_0017091"},{"id":"T17","span":{"begin":1039,"end":1046},"obj":"http://purl.obolibrary.org/obo/GO_0017091"},{"id":"T18","span":{"begin":923,"end":930},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T19","span":{"begin":1039,"end":1046},"obj":"http://purl.obolibrary.org/obo/GO_0005488"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"EDAM-topics","denotations":[{"id":"T1","span":{"begin":341,"end":353},"obj":"http://edamontology.org/topic_0634"},{"id":"T2","span":{"begin":373,"end":378},"obj":"http://edamontology.org/topic_3678"},{"id":"T3","span":{"begin":505,"end":514},"obj":"http://edamontology.org/topic_2830"},{"id":"T4","span":{"begin":1418,"end":1430},"obj":"http://edamontology.org/topic_0634"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"EDAM-DFO","denotations":[{"id":"T1","span":{"begin":260,"end":268},"obj":"http://edamontology.org/data_1756"},{"id":"T2","span":{"begin":354,"end":363},"obj":"http://edamontology.org/operation_2409"},{"id":"T3","span":{"begin":354,"end":363},"obj":"http://edamontology.org/operation_0004"},{"id":"T4","span":{"begin":422,"end":428},"obj":"http://edamontology.org/data_0968"},{"id":"T5","span":{"begin":750,"end":760},"obj":"http://edamontology.org/data_0883"},{"id":"T6","span":{"begin":850,"end":860},"obj":"http://edamontology.org/data_0883"},{"id":"T7","span":{"begin":1163,"end":1175},"obj":"http://edamontology.org/operation_2246"},{"id":"T8","span":{"begin":1228,"end":1236},"obj":"http://edamontology.org/operation_2945"},{"id":"T9","span":{"begin":1368,"end":1373},"obj":"http://edamontology.org/data_0007"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"GlyTouCan-IUPAC","denotations":[{"id":"GlycanIUPAC_T1","span":{"begin":199,"end":203},"obj":"\"http://rdf.glycoinfo.org/glycan/G91489RT\""},{"id":"GlycanIUPAC_T2","span":{"begin":875,"end":879},"obj":"\"http://rdf.glycoinfo.org/glycan/G91489RT\""},{"id":"GlycanIUPAC_T3","span":{"begin":199,"end":203},"obj":"\"http://rdf.glycoinfo.org/glycan/G54004DL\""},{"id":"GlycanIUPAC_T4","span":{"begin":875,"end":879},"obj":"\"http://rdf.glycoinfo.org/glycan/G54004DL\""},{"id":"GlycanIUPAC_T5","span":{"begin":199,"end":203},"obj":"\"http://rdf.glycoinfo.org/glycan/G90208ZS\""},{"id":"GlycanIUPAC_T6","span":{"begin":875,"end":879},"obj":"\"http://rdf.glycoinfo.org/glycan/G90208ZS\""},{"id":"GlycanIUPAC_T7","span":{"begin":199,"end":203},"obj":"\"http://rdf.glycoinfo.org/glycan/G47973RQ\""},{"id":"GlycanIUPAC_T8","span":{"begin":875,"end":879},"obj":"\"http://rdf.glycoinfo.org/glycan/G47973RQ\""},{"id":"GlycanIUPAC_T9","span":{"begin":199,"end":203},"obj":"\"http://rdf.glycoinfo.org/glycan/G35471RR\""},{"id":"GlycanIUPAC_T10","span":{"begin":875,"end":879},"obj":"\"http://rdf.glycoinfo.org/glycan/G35471RR\""},{"id":"GlycanIUPAC_T11","span":{"begin":199,"end":203},"obj":"\"http://rdf.glycoinfo.org/glycan/G45399NV\""},{"id":"GlycanIUPAC_T12","span":{"begin":875,"end":879},"obj":"\"http://rdf.glycoinfo.org/glycan/G45399NV\""},{"id":"GlycanIUPAC_T13","span":{"begin":199,"end":203},"obj":"\"http://rdf.glycoinfo.org/glycan/G52061FU\""},{"id":"GlycanIUPAC_T14","span":{"begin":875,"end":879},"obj":"\"http://rdf.glycoinfo.org/glycan/G52061FU\""},{"id":"GlycanIUPAC_T15","span":{"begin":199,"end":203},"obj":"\"http://rdf.glycoinfo.org/glycan/G10736YH\""},{"id":"GlycanIUPAC_T16","span":{"begin":875,"end":879},"obj":"\"http://rdf.glycoinfo.org/glycan/G10736YH\""},{"id":"GlycanIUPAC_T17","span":{"begin":199,"end":203},"obj":"\"http://rdf.glycoinfo.org/glycan/G30254CI\""},{"id":"GlycanIUPAC_T18","span":{"begin":875,"end":879},"obj":"\"http://rdf.glycoinfo.org/glycan/G30254CI\""},{"id":"GlycanIUPAC_T19","span":{"begin":199,"end":203},"obj":"\"http://rdf.glycoinfo.org/glycan/G57856MN\""},{"id":"GlycanIUPAC_T20","span":{"begin":875,"end":879},"obj":"\"http://rdf.glycoinfo.org/glycan/G57856MN\""},{"id":"GlycanIUPAC_T21","span":{"begin":199,"end":203},"obj":"\"http://rdf.glycoinfo.org/glycan/G95754KF\""},{"id":"GlycanIUPAC_T22","span":{"begin":875,"end":879},"obj":"\"http://rdf.glycoinfo.org/glycan/G95754KF\""},{"id":"GlycanIUPAC_T23","span":{"begin":199,"end":203},"obj":"\"http://rdf.glycoinfo.org/glycan/G90067UQ\""},{"id":"GlycanIUPAC_T24","span":{"begin":875,"end":879},"obj":"\"http://rdf.glycoinfo.org/glycan/G90067UQ\""},{"id":"GlycanIUPAC_T25","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G23425WZ\""},{"id":"GlycanIUPAC_T26","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G23425WZ\""},{"id":"GlycanIUPAC_T27","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G02874VH\""},{"id":"GlycanIUPAC_T28","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G02874VH\""},{"id":"GlycanIUPAC_T29","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G99699DW\""},{"id":"GlycanIUPAC_T30","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G99699DW\""},{"id":"GlycanIUPAC_T31","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G22074RM\""},{"id":"GlycanIUPAC_T32","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G22074RM\""},{"id":"GlycanIUPAC_T33","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G48535VZ\""},{"id":"GlycanIUPAC_T34","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G48535VZ\""},{"id":"GlycanIUPAC_T35","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G39738WL\""},{"id":"GlycanIUPAC_T36","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G39738WL\""},{"id":"GlycanIUPAC_T37","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G42313WU\""},{"id":"GlycanIUPAC_T38","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G42313WU\""},{"id":"GlycanIUPAC_T39","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G00393CK\""},{"id":"GlycanIUPAC_T40","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G00393CK\""},{"id":"GlycanIUPAC_T41","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G42649EX\""},{"id":"GlycanIUPAC_T42","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G42649EX\""},{"id":"GlycanIUPAC_T43","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G46880SB\""},{"id":"GlycanIUPAC_T44","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G46880SB\""},{"id":"GlycanIUPAC_T45","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G75599IR\""},{"id":"GlycanIUPAC_T46","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G75599IR\""},{"id":"GlycanIUPAC_T47","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G58985MU\""},{"id":"GlycanIUPAC_T48","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G58985MU\""},{"id":"GlycanIUPAC_T49","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G92517PO\""},{"id":"GlycanIUPAC_T50","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G92517PO\""},{"id":"GlycanIUPAC_T51","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G88512YL\""},{"id":"GlycanIUPAC_T52","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G88512YL\""},{"id":"GlycanIUPAC_T53","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G41473NX\""},{"id":"GlycanIUPAC_T54","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G41473NX\""},{"id":"GlycanIUPAC_T55","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G71089RB\""},{"id":"GlycanIUPAC_T56","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G71089RB\""},{"id":"GlycanIUPAC_T57","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G73485GZ\""},{"id":"GlycanIUPAC_T58","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G73485GZ\""},{"id":"GlycanIUPAC_T59","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G61406KC\""},{"id":"GlycanIUPAC_T60","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G61406KC\""},{"id":"GlycanIUPAC_T61","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G34412GZ\""},{"id":"GlycanIUPAC_T62","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G34412GZ\""},{"id":"GlycanIUPAC_T63","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G67209FP\""},{"id":"GlycanIUPAC_T64","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G67209FP\""},{"id":"GlycanIUPAC_T65","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G61442IL\""},{"id":"GlycanIUPAC_T66","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G61442IL\""},{"id":"GlycanIUPAC_T67","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G93729MV\""},{"id":"GlycanIUPAC_T68","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G93729MV\""},{"id":"GlycanIUPAC_T69","span":{"begin":252,"end":258},"obj":"\"http://rdf.glycoinfo.org/glycan/G92144AE\""},{"id":"GlycanIUPAC_T70","span":{"begin":716,"end":722},"obj":"\"http://rdf.glycoinfo.org/glycan/G92144AE\""}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"GlycoBiology-Motifs","denotations":[{"id":"T1","span":{"begin":69,"end":80},"obj":"http://rdf.glycoinfo.org/glycan/G00018MO"},{"id":"T2","span":{"begin":92,"end":103},"obj":"http://rdf.glycoinfo.org/glycan/G00018MO"},{"id":"T3","span":{"begin":69,"end":88},"obj":"http://rdf.glycoinfo.org/glycan/G00018MO"},{"id":"T4","span":{"begin":92,"end":111},"obj":"http://rdf.glycoinfo.org/glycan/G00018MO"},{"id":"T5","span":{"begin":577,"end":593},"obj":"http://rdf.glycoinfo.org/glycan/G00019MO"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"Lectin","denotations":[{"id":"Lectin_T1","span":{"begin":664,"end":667},"obj":"https://acgg.asia/db/lfdb/LfDB0192"},{"id":"Lectin_T2","span":{"begin":672,"end":675},"obj":"https://acgg.asia/db/lfdb/LfDB0192"},{"id":"Lectin_T3","span":{"begin":1030,"end":1033},"obj":"https://acgg.asia/db/lfdb/LfDB0192"},{"id":"Lectin_T4","span":{"begin":1145,"end":1148},"obj":"https://acgg.asia/db/lfdb/LfDB0192"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"GlycoBiology-Epitope","denotations":[{"id":"PD-GlycoEpitope-B_T1","span":{"begin":69,"end":80},"obj":"http://www.glycoepitope.jp/epitopes/EP0081"},{"id":"PD-GlycoEpitope-B_T2","span":{"begin":92,"end":103},"obj":"http://www.glycoepitope.jp/epitopes/EP0081"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":664,"end":667},"obj":"Disease"},{"id":"T2","span":{"begin":672,"end":675},"obj":"Disease"},{"id":"T3","span":{"begin":1030,"end":1033},"obj":"Disease"},{"id":"T4","span":{"begin":1145,"end":1148},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0019569"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0019569"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0019569"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MONDO_0019569"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":69,"end":80},"obj":"Glycan"},{"id":"T2","span":{"begin":92,"end":103},"obj":"Glycan"},{"id":"T3","span":{"begin":252,"end":258},"obj":"Glycan"},{"id":"T4","span":{"begin":716,"end":722},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G43702JT"},{"id":"A5","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G43702JT"},{"id":"A6","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G39738WL"},{"id":"A7","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL"},{"id":"A4","pred":"glycosmos_id","subj":"T4","obj":"https://glycosmos.org/glycans/show/G39738WL"},{"id":"A8","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}

{"project":"Glycosmos15-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":69,"end":80},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":92,"end":103},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0081"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0081"}],"text":"Isolation and characterization of monoclonal antibodies specific for chondroitin sulfate E.\nChondroitin sulfate E (CSE) is a polysaccharide containing mainly disaccharide units of D-glucuronic acid (GlcA) and 4,6-O-disulfated N-acetyl-D-galactosamine (GalNAc) residues (E-unit) in the amount of ∼ 60%. CSE is involved in many biological and pathological processes. In this study, we established new monoclonal antibodies, termed E-12C and E-18H, by using CSE that contained more than 70% of E-units as an immunogen. These antibodies recognized CSE but not other CSs isomers or dermatan sulfate (DS). We evaluated the reactivities of the antibodies to 6-O-sulfated CSA (6S-CSA) and DS (6S-DS) that possessed ∼ 60% of GalNAc (4S, 6S) moieties in their structures. Neither of the antibodies reacted with 6S-DS. The antibodies strictly distinguished the structural difference of GlcA and L-iduronic acid in the polysaccharide. Binding affinities of the antibodies were determined by a surface plasmon resonance assay using CSE and 6S-CSA. The binding affinities were strongly associated with the molecular weight of CSE and the E-unit content of 6S-CSA. Moreover, we demonstrated that the antibodies are applicable to histochemical analysis. In conclusion, the new anti-CSE monoclonal antibodies specifically recognize the E-unit of CSE. The antibodies will become useful tools for the investigation of the biological and pathological significance of CSE."}