PubMed:25964111 JSONTXT

{"project":"Glycan-Motif","denotations":[{"id":"T1","span":{"begin":37,"end":50},"obj":"https://glytoucan.org/Structures/Glycans/G17108EX"},{"id":"T2","span":{"begin":37,"end":50},"obj":"https://glytoucan.org/Structures/Glycans/G58507AZ"},{"id":"T3","span":{"begin":545,"end":554},"obj":"https://glytoucan.org/Structures/Glycans/G71832QJ"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":37,"end":50},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":545,"end":554},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G58507AZ"},{"id":"A2","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G17108EX"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G71832QJ"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":179},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":180,"end":318},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":319,"end":555},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":556,"end":739},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":179},"obj":"Sentence"},{"id":"T2","span":{"begin":180,"end":318},"obj":"Sentence"},{"id":"T3","span":{"begin":319,"end":555},"obj":"Sentence"},{"id":"T4","span":{"begin":556,"end":739},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":179},"obj":"Sentence"},{"id":"T2","span":{"begin":180,"end":318},"obj":"Sentence"},{"id":"T3","span":{"begin":319,"end":555},"obj":"Sentence"},{"id":"T4","span":{"begin":556,"end":739},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":37,"end":50},"obj":"https://glytoucan.org/Structures/Glycans/G17108EX"},{"id":"T2","span":{"begin":37,"end":50},"obj":"https://glytoucan.org/Structures/Glycans/G58507AZ"},{"id":"T3","span":{"begin":545,"end":554},"obj":"https://glytoucan.org/Structures/Glycans/G71832QJ"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":37,"end":42},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T2","span":{"begin":37,"end":42},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T3","span":{"begin":142,"end":147},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T4","span":{"begin":142,"end":147},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T5","span":{"begin":263,"end":268},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T6","span":{"begin":263,"end":268},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T7","span":{"begin":533,"end":538},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T8","span":{"begin":533,"end":538},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T9","span":{"begin":702,"end":707},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T10","span":{"begin":702,"end":707},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"GlycoBiology-FMA","denotations":[{"id":"_T1","span":{"begin":37,"end":42},"obj":"FMAID:256053"},{"id":"_T2","span":{"begin":142,"end":147},"obj":"FMAID:256053"},{"id":"_T3","span":{"begin":263,"end":268},"obj":"FMAID:256053"},{"id":"_T4","span":{"begin":330,"end":338},"obj":"FMAID:165656"},{"id":"_T5","span":{"begin":330,"end":338},"obj":"FMAID:67745"},{"id":"_T6","span":{"begin":428,"end":441},"obj":"FMAID:196789"},{"id":"_T7","span":{"begin":428,"end":441},"obj":"FMAID:82794"},{"id":"_T8","span":{"begin":448,"end":473},"obj":"FMAID:82786"},{"id":"_T9","span":{"begin":448,"end":473},"obj":"FMAID:196780"},{"id":"_T10","span":{"begin":500,"end":515},"obj":"FMAID:82742"},{"id":"_T11","span":{"begin":500,"end":515},"obj":"FMAID:196731"},{"id":"_T12","span":{"begin":533,"end":538},"obj":"FMAID:256053"},{"id":"_T13","span":{"begin":635,"end":639},"obj":"FMAID:198663"},{"id":"_T14","span":{"begin":702,"end":707},"obj":"FMAID:256053"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":142,"end":161},"obj":"http://www.uniprot.org/uniprot/Q96PL6"},{"id":"T2","span":{"begin":702,"end":721},"obj":"http://www.uniprot.org/uniprot/Q96PL6"},{"id":"T3","span":{"begin":142,"end":161},"obj":"http://www.uniprot.org/uniprot/B2RUZ4"},{"id":"T4","span":{"begin":702,"end":721},"obj":"http://www.uniprot.org/uniprot/B2RUZ4"},{"id":"T5","span":{"begin":142,"end":161},"obj":"http://www.uniprot.org/uniprot/O75326"},{"id":"T6","span":{"begin":702,"end":721},"obj":"http://www.uniprot.org/uniprot/O75326"},{"id":"T7","span":{"begin":142,"end":161},"obj":"http://www.uniprot.org/uniprot/P35613"},{"id":"T8","span":{"begin":702,"end":721},"obj":"http://www.uniprot.org/uniprot/P35613"},{"id":"T9","span":{"begin":426,"end":441},"obj":"http://www.uniprot.org/uniprot/P16278"},{"id":"T10","span":{"begin":446,"end":473},"obj":"http://www.uniprot.org/uniprot/P17050"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":426,"end":441},"obj":"http://www.uniprot.org/uniprot/P23780"},{"id":"T2","span":{"begin":446,"end":473},"obj":"http://www.uniprot.org/uniprot/Q9QWR8"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":43,"end":48},"obj":"http://purl.bioontology.org/ontology/STY/T096"},{"id":"T2","span":{"begin":148,"end":153},"obj":"http://purl.bioontology.org/ontology/STY/T096"},{"id":"T3","span":{"begin":269,"end":274},"obj":"http://purl.bioontology.org/ontology/STY/T096"},{"id":"T4","span":{"begin":539,"end":544},"obj":"http://purl.bioontology.org/ontology/STY/T096"},{"id":"T5","span":{"begin":708,"end":713},"obj":"http://purl.bioontology.org/ontology/STY/T096"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":15,"end":24},"obj":"http://purl.obolibrary.org/obo/GO_0009058"},{"id":"T2","span":{"begin":199,"end":208},"obj":"http://purl.obolibrary.org/obo/GO_0009058"},{"id":"T3","span":{"begin":71,"end":82},"obj":"http://purl.obolibrary.org/obo/GO_0032502"},{"id":"T4","span":{"begin":343,"end":352},"obj":"http://purl.obolibrary.org/obo/GO_0032502"},{"id":"T5","span":{"begin":360,"end":369},"obj":"http://purl.obolibrary.org/obo/GO_0046960"},{"id":"T6","span":{"begin":448,"end":482},"obj":"http://purl.obolibrary.org/obo/GO_0008456"},{"id":"T7","span":{"begin":448,"end":482},"obj":"http://purl.obolibrary.org/obo/GO_0032428"},{"id":"T8","span":{"begin":625,"end":639},"obj":"http://purl.obolibrary.org/obo/GO_0010467"},{"id":"T9","span":{"begin":625,"end":648},"obj":"http://purl.obolibrary.org/obo/GO_0010468"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"UBERON-AE","denotations":[{"id":"T1","span":{"begin":37,"end":42},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"T2","span":{"begin":142,"end":147},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"T3","span":{"begin":263,"end":268},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"T4","span":{"begin":533,"end":538},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"T5","span":{"begin":702,"end":707},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"GlycoBiology-MAT","denotations":[{"id":"T1","span":{"begin":37,"end":42},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T2","span":{"begin":37,"end":42},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T3","span":{"begin":142,"end":147},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T4","span":{"begin":142,"end":147},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T5","span":{"begin":263,"end":268},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T6","span":{"begin":263,"end":268},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T7","span":{"begin":533,"end":538},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T8","span":{"begin":533,"end":538},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T9","span":{"begin":702,"end":707},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T10","span":{"begin":702,"end":707},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"GlycoBiology-Motifs","denotations":[{"id":"T1","span":{"begin":37,"end":48},"obj":"http://rdf.glycoinfo.org/glycan/G00066MO"},{"id":"T2","span":{"begin":142,"end":153},"obj":"http://rdf.glycoinfo.org/glycan/G00066MO"},{"id":"T3","span":{"begin":263,"end":274},"obj":"http://rdf.glycoinfo.org/glycan/G00066MO"},{"id":"T4","span":{"begin":533,"end":544},"obj":"http://rdf.glycoinfo.org/glycan/G00066MO"},{"id":"T5","span":{"begin":702,"end":713},"obj":"http://rdf.glycoinfo.org/glycan/G00066MO"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"GlycoBiology-Epitope","denotations":[{"id":"PD-GlycoEpitope-B_T1","span":{"begin":28,"end":50},"obj":"http://www.glycoepitope.jp/epitopes/EP0257"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"performance-test","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":37,"end":42},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"PD-UBERON-AE-B_T2","span":{"begin":142,"end":147},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"PD-UBERON-AE-B_T3","span":{"begin":263,"end":268},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"PD-UBERON-AE-B_T4","span":{"begin":533,"end":538},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"PD-UBERON-AE-B_T5","span":{"begin":702,"end":707},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"Glycan-GlyCosmos","denotations":[{"id":"T1","span":{"begin":37,"end":50},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G00066MO"},{"id":"A2","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00066MO"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"GlyCosmos15-HP","denotations":[{"id":"T1","span":{"begin":353,"end":369},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0041092"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"GlyCosmos15-UBERON","denotations":[{"id":"T1","span":{"begin":37,"end":42},"obj":"Body_part"},{"id":"T2","span":{"begin":142,"end":147},"obj":"Body_part"},{"id":"T3","span":{"begin":263,"end":268},"obj":"Body_part"},{"id":"T4","span":{"begin":533,"end":538},"obj":"Body_part"},{"id":"T5","span":{"begin":702,"end":707},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"GlyCosmos15-MAT","denotations":[{"id":"T1","span":{"begin":37,"end":42},"obj":"Body_part"},{"id":"T3","span":{"begin":142,"end":147},"obj":"Body_part"},{"id":"T5","span":{"begin":263,"end":268},"obj":"Body_part"},{"id":"T7","span":{"begin":533,"end":538},"obj":"Body_part"},{"id":"T9","span":{"begin":702,"end":707},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A2","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A3","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A4","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A5","pred":"mat_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A6","pred":"mat_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A7","pred":"mat_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A8","pred":"mat_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A9","pred":"mat_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A10","pred":"mat_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/MAT_0000315"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":179},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":180,"end":318},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":319,"end":555},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":556,"end":739},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":179},"obj":"Sentence"},{"id":"T2","span":{"begin":180,"end":318},"obj":"Sentence"},{"id":"T3","span":{"begin":319,"end":555},"obj":"Sentence"},{"id":"T4","span":{"begin":556,"end":739},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":179},"obj":"Sentence"},{"id":"T2","span":{"begin":180,"end":318},"obj":"Sentence"},{"id":"T3","span":{"begin":319,"end":555},"obj":"Sentence"},{"id":"T4","span":{"begin":556,"end":739},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":179},"obj":"Sentence"},{"id":"T2","span":{"begin":180,"end":318},"obj":"Sentence"},{"id":"T3","span":{"begin":319,"end":555},"obj":"Sentence"},{"id":"T4","span":{"begin":556,"end":739},"obj":"Sentence"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":37,"end":50},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G00066MO"},{"id":"A2","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00066MO"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":37,"end":42},"obj":"Body_part"},{"id":"T2","span":{"begin":142,"end":147},"obj":"Body_part"},{"id":"T3","span":{"begin":263,"end":268},"obj":"Body_part"},{"id":"T4","span":{"begin":533,"end":538},"obj":"Body_part"},{"id":"T5","span":{"begin":702,"end":707},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":37,"end":42},"obj":"Body_part"},{"id":"T3","span":{"begin":142,"end":147},"obj":"Body_part"},{"id":"T5","span":{"begin":263,"end":268},"obj":"Body_part"},{"id":"T7","span":{"begin":533,"end":538},"obj":"Body_part"},{"id":"T9","span":{"begin":702,"end":707},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A2","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A3","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A4","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A5","pred":"mat_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A6","pred":"mat_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A7","pred":"mat_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A8","pred":"mat_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A9","pred":"mat_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A10","pred":"mat_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/MAT_0000315"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}

{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":353,"end":369},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0041092"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}],"text":"Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes.\nA facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes."}