PubMed:25797797 JSONTXT

{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":0,"end":36},"obj":"Gene:732851"},{"id":"T2","span":{"begin":42,"end":50},"obj":"Species:7091"},{"id":"T3","span":{"begin":159,"end":192},"obj":"Gene:100862794"},{"id":"T4","span":{"begin":194,"end":198},"obj":"Gene:100862794"},{"id":"T5","span":{"begin":358,"end":362},"obj":"Gene:100862794"},{"id":"T6","span":{"begin":455,"end":466},"obj":"Species:7091"},{"id":"T7","span":{"begin":533,"end":542},"obj":"Gene:732851"},{"id":"T8","span":{"begin":593,"end":602},"obj":"Gene:32099"},{"id":"T9","span":{"begin":639,"end":648},"obj":"Gene:732851"},{"id":"T10","span":{"begin":660,"end":676},"obj":"Species:562"},{"id":"T11","span":{"begin":682,"end":699},"obj":"Species:1423"},{"id":"T12","span":{"begin":711,"end":718},"obj":"Species:7091"},{"id":"T13","span":{"begin":831,"end":840},"obj":"Gene:732851"},{"id":"T14","span":{"begin":868,"end":875},"obj":"Species:562"},{"id":"T15","span":{"begin":880,"end":892},"obj":"Species:1423"},{"id":"T16","span":{"begin":938,"end":945},"obj":"Species:7091"},{"id":"T17","span":{"begin":965,"end":974},"obj":"Gene:732851"},{"id":"T18","span":{"begin":1063,"end":1069},"obj":"Gene:100124425"},{"id":"T19","span":{"begin":1129,"end":1136},"obj":"Gene:692365"},{"id":"T20","span":{"begin":1165,"end":1174},"obj":"Gene:732851"},{"id":"T21","span":{"begin":1226,"end":1233},"obj":"Species:7091"}],"text":"Peptidoglycan recognition protein S2 from silkworm integument: characterization, microbe-induced expression, and involvement in the immune-deficiency pathway.\nPeptidoglycan recognition protein (PGRP) binds specifically to peptidoglycan and plays an important role as a pattern recognition receptor in the innate immunity of insects. The cDNA of a short-type PGRP, an open reading frame of 588 bp encoding a polypeptide of 196 amino acids, was cloned from Bombyx mori. A phylogenetic tree was constructed, and the results showed that BmPGRP-S2 was most similar to Drosophila melanogaster PGRP (DmPGRP-SA). The induced expression profile of BmPGRP-S2 in healthy Escherichia coli- and Bacillus subtilis-challenged B. mori was measured using semiquantitative reverse transcriptase polymerase chain reaction analysis. The expression of BmPGRP-S2 was upregulated at 24 h by E. coli and Ba. subtilis challenge. In addition, in the integument of B. mori, RNAi knockdown of BmPGRP-S2 caused an obvious reduction in the transcription expression of the transcription factor Relish and in antibacterial effector genes Attacin, Gloverin, and Moricin. The results indicated that BmPGRP-S2 participates in the signal transduction pathway of B. mori."}

{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":0,"end":36},"obj":"Gene:732851"},{"id":"T10","span":{"begin":660,"end":676},"obj":"Species:562"},{"id":"T11","span":{"begin":682,"end":699},"obj":"Species:1423"},{"id":"T12","span":{"begin":711,"end":718},"obj":"Species:7091"},{"id":"T13","span":{"begin":831,"end":840},"obj":"Gene:732851"},{"id":"T14","span":{"begin":868,"end":875},"obj":"Species:562"},{"id":"T15","span":{"begin":880,"end":892},"obj":"Species:1423"},{"id":"T16","span":{"begin":938,"end":945},"obj":"Species:7091"},{"id":"T17","span":{"begin":965,"end":974},"obj":"Gene:732851"},{"id":"T18","span":{"begin":1063,"end":1069},"obj":"Gene:100124425"},{"id":"T19","span":{"begin":1129,"end":1136},"obj":"Gene:692365"},{"id":"T2","span":{"begin":42,"end":50},"obj":"Species:7091"},{"id":"T20","span":{"begin":1165,"end":1174},"obj":"Gene:732851"},{"id":"T21","span":{"begin":1226,"end":1233},"obj":"Species:7091"},{"id":"T3","span":{"begin":159,"end":192},"obj":"Gene:100862794"},{"id":"T4","span":{"begin":194,"end":198},"obj":"Gene:100862794"},{"id":"T5","span":{"begin":358,"end":362},"obj":"Gene:100862794"},{"id":"T6","span":{"begin":455,"end":466},"obj":"Species:7091"},{"id":"T7","span":{"begin":533,"end":542},"obj":"Gene:732851"},{"id":"T8","span":{"begin":593,"end":602},"obj":"Gene:32099"},{"id":"T9","span":{"begin":639,"end":648},"obj":"Gene:732851"}],"text":"Peptidoglycan recognition protein S2 from silkworm integument: characterization, microbe-induced expression, and involvement in the immune-deficiency pathway.\nPeptidoglycan recognition protein (PGRP) binds specifically to peptidoglycan and plays an important role as a pattern recognition receptor in the innate immunity of insects. The cDNA of a short-type PGRP, an open reading frame of 588 bp encoding a polypeptide of 196 amino acids, was cloned from Bombyx mori. A phylogenetic tree was constructed, and the results showed that BmPGRP-S2 was most similar to Drosophila melanogaster PGRP (DmPGRP-SA). The induced expression profile of BmPGRP-S2 in healthy Escherichia coli- and Bacillus subtilis-challenged B. mori was measured using semiquantitative reverse transcriptase polymerase chain reaction analysis. The expression of BmPGRP-S2 was upregulated at 24 h by E. coli and Ba. subtilis challenge. In addition, in the integument of B. mori, RNAi knockdown of BmPGRP-S2 caused an obvious reduction in the transcription expression of the transcription factor Relish and in antibacterial effector genes Attacin, Gloverin, and Moricin. The results indicated that BmPGRP-S2 participates in the signal transduction pathway of B. mori."}