Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-106 |
Sentence |
denotes |
Hypobetalipoproteinemia due to an apolipoprotein B gene exon 21 deletion derived by Alu-Alu recombination. |
T2 |
107-195 |
Sentence |
denotes |
We report the molecular defect in an individual with homozygous hypobetalipoproteinemia. |
T3 |
196-345 |
Sentence |
denotes |
A unique TaqI restriction fragment length polymorphism was found in the midportion of the apolipoprotein B (apoB) gene using the genomic probe, pB51. |
T4 |
346-495 |
Sentence |
denotes |
The probe, which identifies TaqI fragments of 8.4 and 2.8 kilobases (kb) in normal individuals, hybridized to a single 11-kb fragment in the proband. |
T5 |
496-620 |
Sentence |
denotes |
The parents of the proband showed all three TaqI fragments, implying that they are heterozygotes for the mutant apoB allele. |
T6 |
621-815 |
Sentence |
denotes |
In this family, the mutant allele cosegregated with low total cholesterol levels and formal linkage analysis gave a decimal logarithm of the ratio score of 3.3 at a recombination frequency of 0. |
T7 |
816-907 |
Sentence |
denotes |
The polymorphic TaqI site was localized to an EcoRI fragment of 4 kb in normal individuals. |
T8 |
908-1012 |
Sentence |
denotes |
The corresponding fragment in the proband was 3.4 kb, suggesting a 0.6-kb deletion in the mutant allele. |
T9 |
1013-1112 |
Sentence |
denotes |
Both the normal 4-kb EcoRI fragment and the mutant 3.4-kb EcoRI fragment were cloned and sequenced. |
T10 |
1113-1188 |
Sentence |
denotes |
In the normal allele, the 4-kb EcoRI fragment extends from intron 20 to 23. |
T11 |
1189-1258 |
Sentence |
denotes |
Exon 21 is flanked by Alu sequences that are in the same orientation. |
T12 |
1259-1438 |
Sentence |
denotes |
The mutant allele had a 694-bp deletion in this region which included a small part of the Alu sequence in intron 20, the entire exon 21, and most of the Alu sequence in intron 21. |
T13 |
1439-1569 |
Sentence |
denotes |
The polymorphic TaqI site, which lies within the Alu sequence in intron 21, was absent in the proband as a result of the deletion. |
T14 |
1570-1665 |
Sentence |
denotes |
The deletion of exon 21 results in a frame shift mutation and the introduction of a stop codon. |
T15 |
1666-1743 |
Sentence |
denotes |
Translation of the encoded mRNA would yield a prematurely terminated protein. |
T16 |
1744-1852 |
Sentence |
denotes |
This mutant apoB protein would be 1085 amino acids long with the 73 carboxyl-terminal residues out of frame. |
T17 |
1853-2042 |
Sentence |
denotes |
We postulate that the deletion of exon 21 is the consequence of a crossover event between the Alu sequences in introns 20 and 21 resulting in nonreciprocal exchange between two chromosomes. |
T1 |
0-106 |
Sentence |
denotes |
Hypobetalipoproteinemia due to an apolipoprotein B gene exon 21 deletion derived by Alu-Alu recombination. |
T2 |
107-195 |
Sentence |
denotes |
We report the molecular defect in an individual with homozygous hypobetalipoproteinemia. |
T3 |
196-345 |
Sentence |
denotes |
A unique TaqI restriction fragment length polymorphism was found in the midportion of the apolipoprotein B (apoB) gene using the genomic probe, pB51. |
T4 |
346-495 |
Sentence |
denotes |
The probe, which identifies TaqI fragments of 8.4 and 2.8 kilobases (kb) in normal individuals, hybridized to a single 11-kb fragment in the proband. |
T5 |
496-620 |
Sentence |
denotes |
The parents of the proband showed all three TaqI fragments, implying that they are heterozygotes for the mutant apoB allele. |
T6 |
621-815 |
Sentence |
denotes |
In this family, the mutant allele cosegregated with low total cholesterol levels and formal linkage analysis gave a decimal logarithm of the ratio score of 3.3 at a recombination frequency of 0. |
T7 |
816-907 |
Sentence |
denotes |
The polymorphic TaqI site was localized to an EcoRI fragment of 4 kb in normal individuals. |
T8 |
908-1012 |
Sentence |
denotes |
The corresponding fragment in the proband was 3.4 kb, suggesting a 0.6-kb deletion in the mutant allele. |
T9 |
1013-1112 |
Sentence |
denotes |
Both the normal 4-kb EcoRI fragment and the mutant 3.4-kb EcoRI fragment were cloned and sequenced. |
T10 |
1113-1188 |
Sentence |
denotes |
In the normal allele, the 4-kb EcoRI fragment extends from intron 20 to 23. |
T11 |
1189-1258 |
Sentence |
denotes |
Exon 21 is flanked by Alu sequences that are in the same orientation. |
T12 |
1259-1438 |
Sentence |
denotes |
The mutant allele had a 694-bp deletion in this region which included a small part of the Alu sequence in intron 20, the entire exon 21, and most of the Alu sequence in intron 21. |
T13 |
1439-1569 |
Sentence |
denotes |
The polymorphic TaqI site, which lies within the Alu sequence in intron 21, was absent in the proband as a result of the deletion. |
T14 |
1570-1665 |
Sentence |
denotes |
The deletion of exon 21 results in a frame shift mutation and the introduction of a stop codon. |
T15 |
1666-1743 |
Sentence |
denotes |
Translation of the encoded mRNA would yield a prematurely terminated protein. |
T16 |
1744-1852 |
Sentence |
denotes |
This mutant apoB protein would be 1085 amino acids long with the 73 carboxyl-terminal residues out of frame. |
T17 |
1853-2042 |
Sentence |
denotes |
We postulate that the deletion of exon 21 is the consequence of a crossover event between the Alu sequences in introns 20 and 21 resulting in nonreciprocal exchange between two chromosomes. |