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PubMed:2562506 JSONTXT

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GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 412-431 Glycan_Motif denotes N-acetyllactosamine https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00055MO
T2 787-794 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ
T3 870-877 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ
T4 1473-1492 Glycan_Motif denotes N-acetyllactosamine https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00055MO
T5 1835-1854 Glycan_Motif denotes N-acetyllactosamine https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00055MO

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 412-431 https://glytoucan.org/Structures/Glycans/G00055MO denotes N-acetyllactosamine
T2 787-794 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T3 870-877 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T4 1473-1492 https://glytoucan.org/Structures/Glycans/G00055MO denotes N-acetyllactosamine
T5 1835-1854 https://glytoucan.org/Structures/Glycans/G00055MO denotes N-acetyllactosamine

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-153 Sentence denotes Specificity towards oligomannoside and hybrid type glycans of the endo-beta-N-acetylglucosaminidase B from the basidiomycete Sporotrichum dimorphosporum.
TextSentencer_T2 154-574 Sentence denotes We have previously shown that an endo-beta-N-acetylglucosaminidase (EC 3.2.1.96) named "Endo B", isolated from culture filtrates of the basidiomycete Sporotrichum dimorphosporum cleaves asialo-, and to some extent, monosialylated bi-antennary glycans of the N-acetyllactosamine type linked to the asparagine residue of peptide or protein moieties [Bouquelet S, Strecker G, Montreuil J, Spik G (1980) Biochimie 62:43-49].
TextSentencer_T3 575-702 Sentence denotes In the present paper, the substrate specificity of the enzyme towards oligomannoside and hybrid type glycans has been analyzed.
TextSentencer_T4 703-928 Sentence denotes The results obtained indicate that ovalbumin glycopeptides containing four to seven mannose residues and bovine lactotransferrin glycopeptides containing four to nine mannose residues were completely hydrolyzed by the enzyme.
TextSentencer_T5 929-1273 Sentence denotes The degree of cleavage was variable among hybrid type structures, since glycopeptides containing the following glycans: (Gal)1(GlcNAc)3(Man)5(GlcNAc)2; (GlcNAc)3(Man)5(GlcNAc)2;(GlcNAc)3(Man)4(GlcNAc)2 were not hydrolyzed by the enzyme while the percentage of hydrolysis of a glycopeptide containing (GlcNAc)2(Man)5(GlcNAc)2 glycan reached 90%.
TextSentencer_T6 1274-1444 Sentence denotes The bovine lactotransferrin was partially deglycosylated (40%) in the absence of non-ionic detergent while native ovalbumin glycoprotein was not hydrolyzed by the enzyme.
TextSentencer_T7 1445-1613 Sentence denotes The oligomannoside- and the N-acetyllactosamine-type degrading activities present in the culture filtrates were not separated at any step of the purification procedure.
TextSentencer_T8 1614-1767 Sentence denotes Both activities were eluted as a single component with an apparent molecular mass of 89 kDa suggesting that they are located on the same enzyme molecule.
TextSentencer_T9 1768-1909 Sentence denotes Endo B represents a powerful tool for removing oligomannoside- and N-acetyllactosamine-type glycans from N-glycopeptides and N-glycoproteins.
TextSentencer_T10 1910-2086 Sentence denotes Moreover, advantages in the use of Endo B in a soluble form as well as in an immobilized form result in its high activity and in its stability to heat denaturation and storage.
T1 0-153 Sentence denotes Specificity towards oligomannoside and hybrid type glycans of the endo-beta-N-acetylglucosaminidase B from the basidiomycete Sporotrichum dimorphosporum.
T2 154-574 Sentence denotes We have previously shown that an endo-beta-N-acetylglucosaminidase (EC 3.2.1.96) named "Endo B", isolated from culture filtrates of the basidiomycete Sporotrichum dimorphosporum cleaves asialo-, and to some extent, monosialylated bi-antennary glycans of the N-acetyllactosamine type linked to the asparagine residue of peptide or protein moieties [Bouquelet S, Strecker G, Montreuil J, Spik G (1980) Biochimie 62:43-49].
T3 575-702 Sentence denotes In the present paper, the substrate specificity of the enzyme towards oligomannoside and hybrid type glycans has been analyzed.
T4 703-928 Sentence denotes The results obtained indicate that ovalbumin glycopeptides containing four to seven mannose residues and bovine lactotransferrin glycopeptides containing four to nine mannose residues were completely hydrolyzed by the enzyme.
T5 929-1273 Sentence denotes The degree of cleavage was variable among hybrid type structures, since glycopeptides containing the following glycans: (Gal)1(GlcNAc)3(Man)5(GlcNAc)2; (GlcNAc)3(Man)5(GlcNAc)2;(GlcNAc)3(Man)4(GlcNAc)2 were not hydrolyzed by the enzyme while the percentage of hydrolysis of a glycopeptide containing (GlcNAc)2(Man)5(GlcNAc)2 glycan reached 90%.
T6 1274-1444 Sentence denotes The bovine lactotransferrin was partially deglycosylated (40%) in the absence of non-ionic detergent while native ovalbumin glycoprotein was not hydrolyzed by the enzyme.
T7 1445-1613 Sentence denotes The oligomannoside- and the N-acetyllactosamine-type degrading activities present in the culture filtrates were not separated at any step of the purification procedure.
T8 1614-1767 Sentence denotes Both activities were eluted as a single component with an apparent molecular mass of 89 kDa suggesting that they are located on the same enzyme molecule.
T9 1768-1909 Sentence denotes Endo B represents a powerful tool for removing oligomannoside- and N-acetyllactosamine-type glycans from N-glycopeptides and N-glycoproteins.
T10 1910-2086 Sentence denotes Moreover, advantages in the use of Endo B in a soluble form as well as in an immobilized form result in its high activity and in its stability to heat denaturation and storage.

NGLY1-deficiency

Id Subject Object Predicate Lexical cue
PD-NGLY1-deficiency-B_T1 1056-1062 chem:24139 denotes GlcNAc
PD-NGLY1-deficiency-B_T2 1071-1077 chem:24139 denotes GlcNAc
PD-NGLY1-deficiency-B_T3 1082-1088 chem:24139 denotes GlcNAc
PD-NGLY1-deficiency-B_T4 1097-1103 chem:24139 denotes GlcNAc
PD-NGLY1-deficiency-B_T5 1107-1113 chem:24139 denotes GlcNAc
PD-NGLY1-deficiency-B_T6 1122-1128 chem:24139 denotes GlcNAc
PD-NGLY1-deficiency-B_T7 1230-1236 chem:24139 denotes GlcNAc
PD-NGLY1-deficiency-B_T8 1245-1251 chem:24139 denotes GlcNAc
PD-NGLY1-deficiency-B_T9 66-99 hgnc:24622 denotes endo-beta-N-acetylglucosaminidase
PD-NGLY1-deficiency-B_T10 187-220 hgnc:24622 denotes endo-beta-N-acetylglucosaminidase

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 125-152 OrganismTaxon denotes Sporotrichum dimorphosporum 2081349
T2 304-331 OrganismTaxon denotes Sporotrichum dimorphosporum 2081349
T3 808-814 OrganismTaxon denotes bovine 9913
T4 1278-1284 OrganismTaxon denotes bovine 9913