> top > docs > PubMed:25605670 > annotations

PubMed:25605670 JSONTXT

Annnotations TAB JSON ListView MergeView

c_corpus

Id Subject Object Predicate Lexical cue
T3 22-46 D002285 denotes ductal carcinoma in situ
T4 22-46 D002285 denotes ductal carcinoma in situ
T11 91-115 D002285 denotes Ductal carcinoma in situ
T12 91-115 D002285 denotes Ductal carcinoma in situ
T18 117-121 CVCL_5552 denotes DCIS
T17 117-121 D002285 denotes DCIS
T19 117-121 D002285 denotes DCIS
T20 149-155 UBERON:0000310 denotes breast
T21 149-162 D001943 denotes breast cancer
T22 149-162 D001943 denotes breast cancer
T23 187-196 PR:000000230 denotes precursor
T24 187-196 PR:000000291 denotes precursor
T25 229-235 UBERON:0000310 denotes breast
T26 236-243 D009369 denotes cancers
T27 236-243 D009369 denotes cancers
T28 298-308 SO:1000009 denotes transition
T30 312-316 CVCL_5552 denotes DCIS
T29 312-316 D002285 denotes DCIS
T31 312-316 D002285 denotes DCIS
T33 377-381 CVCL_5552 denotes DCIS
T32 377-381 D002285 denotes DCIS
T34 377-381 D002285 denotes DCIS
T35 568-573 D006801 denotes human
T37 574-578 CVCL_5552 denotes DCIS
T36 574-578 D002285 denotes DCIS
T38 574-578 D002285 denotes DCIS
T40 714-718 CVCL_5552 denotes DCIS
T39 714-718 D002285 denotes DCIS
T41 714-718 D002285 denotes DCIS
T42 776-781 D006801 denotes human
T44 782-786 CVCL_5552 denotes DCIS
T43 782-786 D002285 denotes DCIS
T45 782-786 D002285 denotes DCIS
T46 878-887 CHEBI:25367 denotes molecules
T47 892-897 CHEBI:23888 denotes drugs
T49 923-927 CVCL_5552 denotes DCIS
T48 923-927 D002285 denotes DCIS
T50 923-927 D002285 denotes DCIS
T51 975-981 UBERON:0002389 denotes finger
T52 1013-1036 CL:0002327 denotes mammary epithelial cell
T55 1058-1078 GO:0031012 denotes extracellular matrix
T56 1072-1078 UBERON:0027368 denotes matrix
T57 1079-1088 D020100 denotes hydrogels
T58 1079-1088 D020100 denotes hydrogels
T60 1096-1100 CVCL_5552 denotes DCIS
T59 1096-1100 D002285 denotes DCIS
T61 1096-1100 D002285 denotes DCIS
T62 1137-1142 UBERON:0000058 denotes ducts
T64 1155-1159 CVCL_5552 denotes DCIS
T63 1155-1159 D002285 denotes DCIS
T65 1155-1159 D002285 denotes DCIS
T66 1203-1208 D006801 denotes human
T67 1230-1233 CHEBI:412516 denotes HMF
T68 1287-1293 CVCL_E031 denotes center
T69 1313-1317 D002285 denotes DCIS
T71 1313-1317 D002285 denotes DCIS
T70 1313-1317 CVCL_5552 denotes DCIS
T72 1344-1354 SO:1000009 denotes transition
T74 1362-1366 CVCL_5552 denotes DCIS
T73 1362-1366 D002285 denotes DCIS
T75 1362-1366 D002285 denotes DCIS
T77 1547-1551 CVCL_5552 denotes DCIS
T76 1547-1551 D002285 denotes DCIS
T78 1547-1551 D002285 denotes DCIS
T79 1615-1620 D006801 denotes human
T81 1629-1633 CVCL_5552 denotes DCIS
T80 1629-1633 D002285 denotes DCIS
T82 1629-1633 D002285 denotes DCIS
T84 1718-1722 CVCL_5552 denotes DCIS
T83 1718-1722 D002285 denotes DCIS
T85 1718-1722 D002285 denotes DCIS
T86 1735-1744 GO:0009058 denotes formation
T87 1772-1785 GO:0007163 denotes cell polarity
T88 1800-1812 UBERON:0001765 denotes mammary duct
T92 1839-1843 CVCL_5552 denotes DCIS
T91 1839-1843 D002285 denotes DCIS
T93 1839-1843 D002285 denotes DCIS
T94 1892-1895 CHEBI:412516 denotes HMF
T96 1931-1935 CVCL_5552 denotes DCIS
T95 1931-1935 D002285 denotes DCIS
T97 1931-1935 D002285 denotes DCIS
T99 2100-2104 CVCL_5552 denotes DCIS
T98 2100-2104 D002285 denotes DCIS
T100 2100-2104 D002285 denotes DCIS
T101 2123-2128 UBERON:0000058 denotes ducts
T102 2137-2142 UBERON:0000464 denotes lumen
T104 2265-2269 CVCL_5552 denotes DCIS
T103 2265-2269 D002285 denotes DCIS
T105 2265-2269 D002285 denotes DCIS

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 149-162 HP_0003002 denotes breast cancer
T2 149-162 HP_0100013 denotes breast cancer
T3 156-162 HP_0002664 denotes cancer
T4 229-243 HP_0003002 denotes breast cancers
T5 229-243 HP_0100013 denotes breast cancers
T6 236-243 HP_0002664 denotes cancers

UseCases_ArguminSci_Discourse

Id Subject Object Predicate Lexical cue
T1 0-78 DRI_Background denotes Microfluidic model of ductal carcinoma in situ with 3D, organotypic structure.
T2 88-244 DRI_Background denotes D: Ductal carcinoma in situ (DCIS) is a non-invasive form of breast cancer that is thought to be a precursor to most invasive and metastatic breast cancers.
T3 245-398 DRI_Challenge denotes Understanding the mechanisms regulating the invasive transition of DCIS is critical in order to better understand how some types of DCIS become invasive.
T4 399-584 DRI_Background denotes While significant insights have been gained using traditional in vivo and in vitro models, existing models do not adequately recapitulate key structure and functions of human DCIS well.
T5 585-683 DRI_Background denotes In addition, existing models are time-consuming and costly, limiting their use in routine screens.
T6 684-898 DRI_Challenge denotes Here, we present a microscale DCIS model that recapitulates key structures and functions of human DCIS, while enhancing the throughput capability of the system to simultaneously screen numerous molecules and drugs.
T7 908-959 DRI_Approach denotes Our microscale DCIS model is prepared in two steps.
T8 960-1089 DRI_Background denotes First, viscous finger patterning is used to generate mammary epithelial cell-lined lumens through extracellular matrix hydrogels.
T9 1090-1175 DRI_Approach denotes Next, DCIS cells are added to fill the mammary ducts to create a DCIS-like structure.
T10 1176-1318 DRI_Approach denotes For coculture experiments, human mammary fibroblasts (HMF) are added to the two side channels connected to the center channel containing DCIS.
T11 1319-1457 DRI_Outcome denotes To validate the invasive transition of the DCIS model, the invasion of cancer cells and the loss of cell-cell junctions are then examined.
T12 1458-1513 DRI_Unspecified denotes A student t-test is conducted for statistical analysis.
T13 1523-1723 DRI_Outcome denotes We demonstrate that our DCIS model faithfully recapitulates key structures and functions of human mammary DCIS and can be employed to study the mechanisms involved in the invasive progression of DCIS.
T14 1724-1868 DRI_Background denotes First, the formation of cell-cell junctions and cell polarity in the normal mammary duct, and the structure of the DCIS model are characterized.
T15 1869-1936 DRI_Background denotes Second, coculture with HMF is shown to induce the invasion of DCIS.
T16 1937-2013 DRI_Outcome denotes Third, multiple endpoint analyses are demonstrated to validate the invasion.
T17 2027-2154 DRI_Outcome denotes We have developed and characterized a novel in vitro model of normal and DCIS-inflicted mammary ducts with 3D lumen structures.
T18 2155-2302 DRI_Background denotes These models will enable researchers to investigate the role of microenvironmental factors on the invasion of DCIS in more in vivo-like conditions.

PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 91-898 BACKGROUND denotes Ductal carcinoma in situ (DCIS) is a non-invasive form of breast cancer that is thought to be a precursor to most invasive and metastatic breast cancers. Understanding the mechanisms regulating the invasive transition of DCIS is critical in order to better understand how some types of DCIS become invasive. While significant insights have been gained using traditional in vivo and in vitro models, existing models do not adequately recapitulate key structure and functions of human DCIS well. In addition, existing models are time-consuming and costly, limiting their use in routine screens. Here, we present a microscale DCIS model that recapitulates key structures and functions of human DCIS, while enhancing the throughput capability of the system to simultaneously screen numerous molecules and drugs.
T2 908-1513 METHODS denotes Our microscale DCIS model is prepared in two steps. First, viscous finger patterning is used to generate mammary epithelial cell-lined lumens through extracellular matrix hydrogels. Next, DCIS cells are added to fill the mammary ducts to create a DCIS-like structure. For coculture experiments, human mammary fibroblasts (HMF) are added to the two side channels connected to the center channel containing DCIS. To validate the invasive transition of the DCIS model, the invasion of cancer cells and the loss of cell-cell junctions are then examined. A student t-test is conducted for statistical analysis.
T3 1523-2013 RESULTS denotes We demonstrate that our DCIS model faithfully recapitulates key structures and functions of human mammary DCIS and can be employed to study the mechanisms involved in the invasive progression of DCIS. First, the formation of cell-cell junctions and cell polarity in the normal mammary duct, and the structure of the DCIS model are characterized. Second, coculture with HMF is shown to induce the invasion of DCIS. Third, multiple endpoint analyses are demonstrated to validate the invasion.
T4 2027-2302 CONCLUSIONS denotes We have developed and characterized a novel in vitro model of normal and DCIS-inflicted mammary ducts with 3D lumen structures. These models will enable researchers to investigate the role of microenvironmental factors on the invasion of DCIS in more in vivo-like conditions.