PubMed:25580615 JSONTXT

{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":15,"end":22},"obj":"Gene:2006"},{"id":"T10","span":{"begin":1256,"end":1261},"obj":"Species:9606"},{"id":"T11","span":{"begin":1283,"end":1288},"obj":"Species:9606"},{"id":"T12","span":{"begin":1363,"end":1366},"obj":"Species:9823"},{"id":"T2","span":{"begin":112,"end":115},"obj":"Gene:733061"},{"id":"T3","span":{"begin":144,"end":155},"obj":"Species:7091"},{"id":"T4","span":{"begin":333,"end":336},"obj":"Gene:733061"},{"id":"T5","span":{"begin":377,"end":384},"obj":"Gene:2006"},{"id":"T6","span":{"begin":625,"end":628},"obj":"Gene:733061"},{"id":"T7","span":{"begin":690,"end":701},"obj":"Chemical:MESH:C030544"},{"id":"T8","span":{"begin":832,"end":835},"obj":"Gene:733061"},{"id":"T9","span":{"begin":1022,"end":1028},"obj":"Species:4932"}],"text":"Development of elastin-like recombinamer films with antimicrobial activity.\nIn the present work we explored the ABP-CM4 peptide properties from Bombyx mori for the creation of biopolymers with broad antimicrobial activity. An antimicrobial recombinant protein-based polymer (rPBP) was designed by cloning the DNA sequence coding for ABP-CM4 in frame with the N-terminus of the elastin-like recombinamer consisting of 200 repetitions of the pentamer VPAVG, here named A200. The new rPBP, named CM4-A200, was purified via a simplified nonchromatographic method, making use of the thermoresponsive behavior of the A200 polymer. ABP-CM4 peptide was also purified through the incorporation of a formic acid cleavage site between the peptide and the A200 sequence. In soluble state the antimicrobial activity of both CM4-A200 polymer and ABP-CM4 peptide was poorly effective. However, when the CM4-A200 polymer was processed into free-standing films high antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi was observed. The antimicrobial activity of CM4-A200 was dependent on the physical contact of cells with the film surface. Furthermore, CM4-A200 films did not reveal a cytotoxic effect against both normal human skin fibroblasts and human keratinocytes. Finally, we have developed an optimized ex vivo assay with pig skin demonstrating the antimicrobial properties of the CM4-A200 cast films for skin applications."}

{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":15,"end":22},"obj":"Gene:2006"},{"id":"T2","span":{"begin":112,"end":115},"obj":"Gene:733061"},{"id":"T3","span":{"begin":144,"end":155},"obj":"Species:7091"},{"id":"T4","span":{"begin":333,"end":336},"obj":"Gene:733061"},{"id":"T5","span":{"begin":377,"end":384},"obj":"Gene:2006"},{"id":"T6","span":{"begin":625,"end":628},"obj":"Gene:733061"},{"id":"T7","span":{"begin":690,"end":701},"obj":"Chemical:MESH:C030544"},{"id":"T8","span":{"begin":832,"end":835},"obj":"Gene:733061"},{"id":"T9","span":{"begin":1022,"end":1028},"obj":"Species:4932"},{"id":"T10","span":{"begin":1256,"end":1261},"obj":"Species:9606"},{"id":"T11","span":{"begin":1283,"end":1288},"obj":"Species:9606"},{"id":"T12","span":{"begin":1363,"end":1366},"obj":"Species:9823"}],"text":"Development of elastin-like recombinamer films with antimicrobial activity.\nIn the present work we explored the ABP-CM4 peptide properties from Bombyx mori for the creation of biopolymers with broad antimicrobial activity. An antimicrobial recombinant protein-based polymer (rPBP) was designed by cloning the DNA sequence coding for ABP-CM4 in frame with the N-terminus of the elastin-like recombinamer consisting of 200 repetitions of the pentamer VPAVG, here named A200. The new rPBP, named CM4-A200, was purified via a simplified nonchromatographic method, making use of the thermoresponsive behavior of the A200 polymer. ABP-CM4 peptide was also purified through the incorporation of a formic acid cleavage site between the peptide and the A200 sequence. In soluble state the antimicrobial activity of both CM4-A200 polymer and ABP-CM4 peptide was poorly effective. However, when the CM4-A200 polymer was processed into free-standing films high antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi was observed. The antimicrobial activity of CM4-A200 was dependent on the physical contact of cells with the film surface. Furthermore, CM4-A200 films did not reveal a cytotoxic effect against both normal human skin fibroblasts and human keratinocytes. Finally, we have developed an optimized ex vivo assay with pig skin demonstrating the antimicrobial properties of the CM4-A200 cast films for skin applications."}