PubMed:25524297
Annnotations
silkwormbase
{"project":"silkwormbase","denotations":[{"id":"T71","span":{"begin":1955,"end":1959},"obj":"BMO_00114"},{"id":"T73","span":{"begin":2042,"end":2046},"obj":"BMO_00114"},{"id":"T1","span":{"begin":44,"end":50},"obj":"Chemical:MESH:D012694"},{"id":"T10","span":{"begin":420,"end":431},"obj":"Species:7091"},{"id":"T11","span":{"begin":455,"end":459},"obj":"Gene:692767"},{"id":"T12","span":{"begin":526,"end":532},"obj":"Chemical:MESH:D012694"},{"id":"T13","span":{"begin":560,"end":564},"obj":"Gene:692767"},{"id":"T14","span":{"begin":603,"end":606},"obj":"Gene:692466"},{"id":"T15","span":{"begin":680,"end":684},"obj":"Gene:692767"},{"id":"T16","span":{"begin":803,"end":809},"obj":"Chemical:MESH:D012694"},{"id":"T17","span":{"begin":814,"end":818},"obj":"Gene:692767"},{"id":"T18","span":{"begin":898,"end":902},"obj":"Gene:692767"},{"id":"T19","span":{"begin":1059,"end":1063},"obj":"Gene:692767"},{"id":"T2","span":{"begin":57,"end":61},"obj":"Gene:692767"},{"id":"T20","span":{"begin":1105,"end":1109},"obj":"Gene:692767"},{"id":"T21","span":{"begin":1171,"end":1177},"obj":"Chemical:MESH:D000069285"},{"id":"T22","span":{"begin":1183,"end":1189},"obj":"Chemical:MESH:D012965"},{"id":"T23","span":{"begin":1354,"end":1360},"obj":"Chemical:MESH:D000069285"},{"id":"T24","span":{"begin":1383,"end":1389},"obj":"Chemical:MESH:D000001"},{"id":"T25","span":{"begin":1393,"end":1405},"obj":"Chemical:MESH:D019284"},{"id":"T26","span":{"begin":1460,"end":1466},"obj":"Chemical:MESH:D012694"},{"id":"T27","span":{"begin":1495,"end":1501},"obj":"Chemical:MESH:D000069285"},{"id":"T28","span":{"begin":1557,"end":1561},"obj":"Gene:692767"},{"id":"T29","span":{"begin":1576,"end":1580},"obj":"Gene:692767"},{"id":"T3","span":{"begin":99,"end":107},"obj":"Species:7091"},{"id":"T30","span":{"begin":1600,"end":1602},"obj":"Chemical:MESH:C012616"},{"id":"T31","span":{"begin":1644,"end":1649},"obj":"Chemical:MESH:C113580"},{"id":"T32","span":{"begin":1702,"end":1705},"obj":"Gene:692466"},{"id":"T33","span":{"begin":1760,"end":1763},"obj":"Gene:692466"},{"id":"T34","span":{"begin":1792,"end":1800},"obj":"Chemical:MESH:C085911"},{"id":"T35","span":{"begin":1864,"end":1868},"obj":"Gene:692767"},{"id":"T36","span":{"begin":1955,"end":1959},"obj":"Gene:692767"},{"id":"T37","span":{"begin":2042,"end":2046},"obj":"Gene:692767"},{"id":"T38","span":{"begin":2088,"end":2094},"obj":"Chemical:MESH:D012694"},{"id":"T39","span":{"begin":2113,"end":2119},"obj":"Chemical:MESH:D000069285"},{"id":"T4","span":{"begin":109,"end":120},"obj":"Species:7091"},{"id":"T40","span":{"begin":2120,"end":2123},"obj":"Gene:692466"},{"id":"T41","span":{"begin":2137,"end":2144},"obj":"Species:7091"},{"id":"T5","span":{"begin":204,"end":208},"obj":"Gene:692767"},{"id":"T6","span":{"begin":271,"end":274},"obj":"Chemical:-"},{"id":"T7","span":{"begin":350,"end":387},"obj":"Gene:692466"},{"id":"T8","span":{"begin":389,"end":392},"obj":"Gene:692466"},{"id":"T9","span":{"begin":397,"end":401},"obj":"Gene:692767"},{"id":"T43","span":{"begin":57,"end":61},"obj":"BMO_00114"},{"id":"T47","span":{"begin":176,"end":202},"obj":"BMO_00114"},{"id":"T48","span":{"begin":204,"end":208},"obj":"BMO_00114"},{"id":"T49","span":{"begin":397,"end":401},"obj":"BMO_00114"},{"id":"T50","span":{"begin":455,"end":459},"obj":"BMO_00114"},{"id":"T52","span":{"begin":560,"end":564},"obj":"BMO_00114"},{"id":"T53","span":{"begin":680,"end":684},"obj":"BMO_00114"},{"id":"T55","span":{"begin":814,"end":818},"obj":"BMO_00114"},{"id":"T57","span":{"begin":898,"end":902},"obj":"BMO_00114"},{"id":"T60","span":{"begin":1059,"end":1063},"obj":"BMO_00114"},{"id":"T61","span":{"begin":1105,"end":1109},"obj":"BMO_00114"},{"id":"T66","span":{"begin":1557,"end":1561},"obj":"BMO_00114"},{"id":"T67","span":{"begin":1576,"end":1580},"obj":"BMO_00114"},{"id":"T69","span":{"begin":1864,"end":1868},"obj":"BMO_00114"}],"text":"Regulation of histone H3 phosphorylation at serine 10 in PTTH-stimulated prothoracic glands of the silkworm, Bombyx mori.\nA complex signaling network appears to be involved in prothoracicotropic hormone (PTTH)-stimulated ecdysteroidogenesis in insect prothoracic glands (PGs). In the present study, we investigated the localization of phosphorylated extracellular signal-regulated kinase (ERK) in PTTH-stimulated PGs in Bombyx mori. The nuclear effect of PTTH was further studied by examining phosphorylation of histone H3 at serine 10. Results showed that in PTTH-stimulated PGs, higher phosphorylated ERK was detected in nuclear fraction compared to that in cytosolic fraction. PTTH treatment in vitro appears to rapidly enhance the transcriptional activation-associated histone H3 phosphorylation at serine 10. PTTH stimulated histone H3 phosphorylation in a time-dependent manner. Injection of PTTH into day-6 last instar larvae greatly increased histone H3 phosphorylation, verifying the in vitro effect. The stimulation of histone H3 phosphorylation by PTTH appears to be developmentally regulated. PTTH-stimulated histone H3 phosphorylation was greatly reduced in Ca(2+)-free saline or by pretreatment with a potent and specific inhibitor of phospholipase C (PLC), U73122. When PGs were treated with agents that directly elevate the intracellular Ca(2+) concentration (either A23187 or thapsigargin), a greatly increase in histone H3 phosphorylation at serine 10 was observed, indicating Ca(2+)-dependency of histone H3 phosphorylation stimulated by PTTH. In addition, PTTH-stimulated histone H3 phosphorylation was partially reduced by U0126, a specific mitogen-activated protein kinase (MAPK)/ERK kinase (MEK) inhibitor, indicating the involvement of ERK. However, pretreatment with LY294002, a phosphoinositide 3-kinase (PI3K) inhibitor, did not inhibit PTTH-stimulated histone H3 phosphorylation, implying that PI3K signaling is not related to PTTH-stimulated histone H3 phosphorylation. Taken together, these results suggest that PTTH-stimulated histone H3 phosphorylation at serine 10 is mediated by Ca(2+)/ERK signaling in B. mori PGs."}
silkworm_phenotype
{"project":"silkworm_phenotype","denotations":[{"id":"T73","span":{"begin":2042,"end":2046},"obj":"BMO_00114"},{"id":"T71","span":{"begin":1955,"end":1959},"obj":"BMO_00114"},{"id":"T69","span":{"begin":1864,"end":1868},"obj":"BMO_00114"},{"id":"T67","span":{"begin":1576,"end":1580},"obj":"BMO_00114"},{"id":"T66","span":{"begin":1557,"end":1561},"obj":"BMO_00114"},{"id":"T61","span":{"begin":1105,"end":1109},"obj":"BMO_00114"},{"id":"T60","span":{"begin":1059,"end":1063},"obj":"BMO_00114"},{"id":"T57","span":{"begin":898,"end":902},"obj":"BMO_00114"},{"id":"T55","span":{"begin":814,"end":818},"obj":"BMO_00114"},{"id":"T53","span":{"begin":680,"end":684},"obj":"BMO_00114"},{"id":"T52","span":{"begin":560,"end":564},"obj":"BMO_00114"},{"id":"T50","span":{"begin":455,"end":459},"obj":"BMO_00114"},{"id":"T49","span":{"begin":397,"end":401},"obj":"BMO_00114"},{"id":"T48","span":{"begin":204,"end":208},"obj":"BMO_00114"},{"id":"T47","span":{"begin":176,"end":202},"obj":"BMO_00114"},{"id":"T43","span":{"begin":57,"end":61},"obj":"BMO_00114"},{"id":"T9","span":{"begin":397,"end":401},"obj":"Gene:692767"},{"id":"T8","span":{"begin":389,"end":392},"obj":"Gene:692466"},{"id":"T7","span":{"begin":350,"end":387},"obj":"Gene:692466"},{"id":"T6","span":{"begin":271,"end":274},"obj":"Chemical:-"},{"id":"T5","span":{"begin":204,"end":208},"obj":"Gene:692767"},{"id":"T41","span":{"begin":2137,"end":2144},"obj":"Species:7091"},{"id":"T40","span":{"begin":2120,"end":2123},"obj":"Gene:692466"},{"id":"T4","span":{"begin":109,"end":120},"obj":"Species:7091"},{"id":"T39","span":{"begin":2113,"end":2119},"obj":"Chemical:MESH:D000069285"},{"id":"T38","span":{"begin":2088,"end":2094},"obj":"Chemical:MESH:D012694"},{"id":"T37","span":{"begin":2042,"end":2046},"obj":"Gene:692767"},{"id":"T36","span":{"begin":1955,"end":1959},"obj":"Gene:692767"},{"id":"T35","span":{"begin":1864,"end":1868},"obj":"Gene:692767"},{"id":"T34","span":{"begin":1792,"end":1800},"obj":"Chemical:MESH:C085911"},{"id":"T33","span":{"begin":1760,"end":1763},"obj":"Gene:692466"},{"id":"T32","span":{"begin":1702,"end":1705},"obj":"Gene:692466"},{"id":"T31","span":{"begin":1644,"end":1649},"obj":"Chemical:MESH:C113580"},{"id":"T30","span":{"begin":1600,"end":1602},"obj":"Chemical:MESH:C012616"},{"id":"T3","span":{"begin":99,"end":107},"obj":"Species:7091"},{"id":"T29","span":{"begin":1576,"end":1580},"obj":"Gene:692767"},{"id":"T28","span":{"begin":1557,"end":1561},"obj":"Gene:692767"},{"id":"T27","span":{"begin":1495,"end":1501},"obj":"Chemical:MESH:D000069285"},{"id":"T26","span":{"begin":1460,"end":1466},"obj":"Chemical:MESH:D012694"},{"id":"T25","span":{"begin":1393,"end":1405},"obj":"Chemical:MESH:D019284"},{"id":"T24","span":{"begin":1383,"end":1389},"obj":"Chemical:MESH:D000001"},{"id":"T23","span":{"begin":1354,"end":1360},"obj":"Chemical:MESH:D000069285"},{"id":"T22","span":{"begin":1183,"end":1189},"obj":"Chemical:MESH:D012965"},{"id":"T21","span":{"begin":1171,"end":1177},"obj":"Chemical:MESH:D000069285"},{"id":"T20","span":{"begin":1105,"end":1109},"obj":"Gene:692767"},{"id":"T2","span":{"begin":57,"end":61},"obj":"Gene:692767"},{"id":"T19","span":{"begin":1059,"end":1063},"obj":"Gene:692767"},{"id":"T18","span":{"begin":898,"end":902},"obj":"Gene:692767"},{"id":"T17","span":{"begin":814,"end":818},"obj":"Gene:692767"},{"id":"T16","span":{"begin":803,"end":809},"obj":"Chemical:MESH:D012694"},{"id":"T15","span":{"begin":680,"end":684},"obj":"Gene:692767"},{"id":"T14","span":{"begin":603,"end":606},"obj":"Gene:692466"},{"id":"T13","span":{"begin":560,"end":564},"obj":"Gene:692767"},{"id":"T12","span":{"begin":526,"end":532},"obj":"Chemical:MESH:D012694"},{"id":"T11","span":{"begin":455,"end":459},"obj":"Gene:692767"},{"id":"T10","span":{"begin":420,"end":431},"obj":"Species:7091"},{"id":"T1","span":{"begin":44,"end":50},"obj":"Chemical:MESH:D012694"}],"text":"Regulation of histone H3 phosphorylation at serine 10 in PTTH-stimulated prothoracic glands of the silkworm, Bombyx mori.\nA complex signaling network appears to be involved in prothoracicotropic hormone (PTTH)-stimulated ecdysteroidogenesis in insect prothoracic glands (PGs). In the present study, we investigated the localization of phosphorylated extracellular signal-regulated kinase (ERK) in PTTH-stimulated PGs in Bombyx mori. The nuclear effect of PTTH was further studied by examining phosphorylation of histone H3 at serine 10. Results showed that in PTTH-stimulated PGs, higher phosphorylated ERK was detected in nuclear fraction compared to that in cytosolic fraction. PTTH treatment in vitro appears to rapidly enhance the transcriptional activation-associated histone H3 phosphorylation at serine 10. PTTH stimulated histone H3 phosphorylation in a time-dependent manner. Injection of PTTH into day-6 last instar larvae greatly increased histone H3 phosphorylation, verifying the in vitro effect. The stimulation of histone H3 phosphorylation by PTTH appears to be developmentally regulated. PTTH-stimulated histone H3 phosphorylation was greatly reduced in Ca(2+)-free saline or by pretreatment with a potent and specific inhibitor of phospholipase C (PLC), U73122. When PGs were treated with agents that directly elevate the intracellular Ca(2+) concentration (either A23187 or thapsigargin), a greatly increase in histone H3 phosphorylation at serine 10 was observed, indicating Ca(2+)-dependency of histone H3 phosphorylation stimulated by PTTH. In addition, PTTH-stimulated histone H3 phosphorylation was partially reduced by U0126, a specific mitogen-activated protein kinase (MAPK)/ERK kinase (MEK) inhibitor, indicating the involvement of ERK. However, pretreatment with LY294002, a phosphoinositide 3-kinase (PI3K) inhibitor, did not inhibit PTTH-stimulated histone H3 phosphorylation, implying that PI3K signaling is not related to PTTH-stimulated histone H3 phosphorylation. Taken together, these results suggest that PTTH-stimulated histone H3 phosphorylation at serine 10 is mediated by Ca(2+)/ERK signaling in B. mori PGs."}
silkworm
{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":44,"end":50},"obj":"Chemical:MESH:D012694"},{"id":"T2","span":{"begin":57,"end":61},"obj":"Gene:692767"},{"id":"T3","span":{"begin":99,"end":107},"obj":"Species:7091"},{"id":"T4","span":{"begin":109,"end":120},"obj":"Species:7091"},{"id":"T5","span":{"begin":204,"end":208},"obj":"Gene:692767"},{"id":"T6","span":{"begin":271,"end":274},"obj":"Chemical:-"},{"id":"T7","span":{"begin":350,"end":387},"obj":"Gene:692466"},{"id":"T8","span":{"begin":389,"end":392},"obj":"Gene:692466"},{"id":"T9","span":{"begin":397,"end":401},"obj":"Gene:692767"},{"id":"T10","span":{"begin":420,"end":431},"obj":"Species:7091"},{"id":"T11","span":{"begin":455,"end":459},"obj":"Gene:692767"},{"id":"T12","span":{"begin":526,"end":532},"obj":"Chemical:MESH:D012694"},{"id":"T13","span":{"begin":560,"end":564},"obj":"Gene:692767"},{"id":"T14","span":{"begin":603,"end":606},"obj":"Gene:692466"},{"id":"T15","span":{"begin":680,"end":684},"obj":"Gene:692767"},{"id":"T16","span":{"begin":803,"end":809},"obj":"Chemical:MESH:D012694"},{"id":"T17","span":{"begin":814,"end":818},"obj":"Gene:692767"},{"id":"T18","span":{"begin":898,"end":902},"obj":"Gene:692767"},{"id":"T19","span":{"begin":1059,"end":1063},"obj":"Gene:692767"},{"id":"T20","span":{"begin":1105,"end":1109},"obj":"Gene:692767"},{"id":"T21","span":{"begin":1171,"end":1177},"obj":"Chemical:MESH:D000069285"},{"id":"T22","span":{"begin":1183,"end":1189},"obj":"Chemical:MESH:D012965"},{"id":"T23","span":{"begin":1354,"end":1360},"obj":"Chemical:MESH:D000069285"},{"id":"T24","span":{"begin":1383,"end":1389},"obj":"Chemical:MESH:D000001"},{"id":"T25","span":{"begin":1393,"end":1405},"obj":"Chemical:MESH:D019284"},{"id":"T26","span":{"begin":1460,"end":1466},"obj":"Chemical:MESH:D012694"},{"id":"T27","span":{"begin":1495,"end":1501},"obj":"Chemical:MESH:D000069285"},{"id":"T28","span":{"begin":1557,"end":1561},"obj":"Gene:692767"},{"id":"T29","span":{"begin":1576,"end":1580},"obj":"Gene:692767"},{"id":"T30","span":{"begin":1600,"end":1602},"obj":"Chemical:MESH:C012616"},{"id":"T31","span":{"begin":1644,"end":1649},"obj":"Chemical:MESH:C113580"},{"id":"T32","span":{"begin":1702,"end":1705},"obj":"Gene:692466"},{"id":"T33","span":{"begin":1760,"end":1763},"obj":"Gene:692466"},{"id":"T34","span":{"begin":1792,"end":1800},"obj":"Chemical:MESH:C085911"},{"id":"T35","span":{"begin":1864,"end":1868},"obj":"Gene:692767"},{"id":"T36","span":{"begin":1955,"end":1959},"obj":"Gene:692767"},{"id":"T37","span":{"begin":2042,"end":2046},"obj":"Gene:692767"},{"id":"T38","span":{"begin":2088,"end":2094},"obj":"Chemical:MESH:D012694"},{"id":"T39","span":{"begin":2113,"end":2119},"obj":"Chemical:MESH:D000069285"},{"id":"T40","span":{"begin":2120,"end":2123},"obj":"Gene:692466"},{"id":"T41","span":{"begin":2137,"end":2144},"obj":"Species:7091"}],"text":"Regulation of histone H3 phosphorylation at serine 10 in PTTH-stimulated prothoracic glands of the silkworm, Bombyx mori.\nA complex signaling network appears to be involved in prothoracicotropic hormone (PTTH)-stimulated ecdysteroidogenesis in insect prothoracic glands (PGs). In the present study, we investigated the localization of phosphorylated extracellular signal-regulated kinase (ERK) in PTTH-stimulated PGs in Bombyx mori. The nuclear effect of PTTH was further studied by examining phosphorylation of histone H3 at serine 10. Results showed that in PTTH-stimulated PGs, higher phosphorylated ERK was detected in nuclear fraction compared to that in cytosolic fraction. PTTH treatment in vitro appears to rapidly enhance the transcriptional activation-associated histone H3 phosphorylation at serine 10. PTTH stimulated histone H3 phosphorylation in a time-dependent manner. Injection of PTTH into day-6 last instar larvae greatly increased histone H3 phosphorylation, verifying the in vitro effect. The stimulation of histone H3 phosphorylation by PTTH appears to be developmentally regulated. PTTH-stimulated histone H3 phosphorylation was greatly reduced in Ca(2+)-free saline or by pretreatment with a potent and specific inhibitor of phospholipase C (PLC), U73122. When PGs were treated with agents that directly elevate the intracellular Ca(2+) concentration (either A23187 or thapsigargin), a greatly increase in histone H3 phosphorylation at serine 10 was observed, indicating Ca(2+)-dependency of histone H3 phosphorylation stimulated by PTTH. In addition, PTTH-stimulated histone H3 phosphorylation was partially reduced by U0126, a specific mitogen-activated protein kinase (MAPK)/ERK kinase (MEK) inhibitor, indicating the involvement of ERK. However, pretreatment with LY294002, a phosphoinositide 3-kinase (PI3K) inhibitor, did not inhibit PTTH-stimulated histone H3 phosphorylation, implying that PI3K signaling is not related to PTTH-stimulated histone H3 phosphorylation. Taken together, these results suggest that PTTH-stimulated histone H3 phosphorylation at serine 10 is mediated by Ca(2+)/ERK signaling in B. mori PGs."}