PubMed:25497339 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/25497339","sourcedb":"PubMed","sourceid":"25497339","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/25497339","text":"Structural investigation of a uronic acid-containing polysaccharide from abalone by graded acid hydrolysis followed by PMP-HPLC-MSn and NMR analysis.\nA new strategy was applied to elucidate the structure of a polysaccharide from abalone gonad (AGSP). It was hydrolyzed by 0.05 M, 0.2 M, 0.5 M, and 2.0 M TFA at 100 °C for 1 h, sequentially. Every hydrolysate was ultrafiltrated (3000 Da) to collect oligo- and monosaccharides, and the final retentate was further hydrolyzed with 2.0 M TFA at 110 °C and 121 °C for 2 h, respectively. 1-Phenyl-3-methyl-5-pyrazolone (PMP) derivatization followed by HPLC-MSn analysis was applied to detect the sugar residues in these hydrolysates, which allowed proposing their location in the polysaccharide structure. The retentate after 0.5 M TFA hydrolysis was confirmed as the polysaccharide backbone, and it was further analyzed by 1D and 2D NMR spectroscopy. Thus, the structural elucidation of AGSP was accomplished, and it has a backbone of →4)-β-GlcA(1→2)-α-Man(1→ repeating unit with Fuc, Xyl and Gal in the branch. The analytical strategy demonstrated was useful to characterize the structure of polysaccharides.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":149},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":150,"end":250},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":251,"end":340},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":341,"end":532},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":533,"end":750},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":751,"end":896},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":897,"end":1057},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1058,"end":1155},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":149},"obj":"Sentence"},{"id":"T2","span":{"begin":150,"end":250},"obj":"Sentence"},{"id":"T3","span":{"begin":251,"end":340},"obj":"Sentence"},{"id":"T4","span":{"begin":341,"end":532},"obj":"Sentence"},{"id":"T5","span":{"begin":533,"end":750},"obj":"Sentence"},{"id":"T6","span":{"begin":751,"end":896},"obj":"Sentence"},{"id":"T7","span":{"begin":897,"end":1057},"obj":"Sentence"},{"id":"T8","span":{"begin":1058,"end":1155},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":73,"end":80},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":229,"end":236},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"6453"},{"id":"A2","pred":"db_id","subj":"T2","obj":"6453"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"}]},{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":119,"end":122},"obj":"Disease"},{"id":"T2","span":{"begin":565,"end":568},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0017048"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0017048"},{"subj":"T1","pred":"source","obj":"mondo_disease"},{"subj":"T2","pred":"source","obj":"mondo_disease"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":237,"end":242},"obj":"Body_part"},{"id":"T2","span":{"begin":828,"end":836},"obj":"Body_part"},{"id":"T3","span":{"begin":969,"end":977},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000991"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0001130"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0001130"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T2","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T3","pred":"source","obj":"Anatomy-UBERON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#93d4ec","default":true},{"id":"NCBITAXON","color":"#ecea93"},{"id":"mondo_disease","color":"#d093ec"},{"id":"Anatomy-UBERON","color":"#93ecb6"}]}]}}