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GlyCosmos6-UBERON

Id Subject Object Predicate Lexical cue
T1 175-182 Body_part denotes surface
T2 783-788 Body_part denotes blood
T3 838-844 Body_part denotes spleen
T4 953-959 Body_part denotes spleen
T5 972-977 Body_part denotes liver
T6 1169-1173 Body_part denotes cell
T7 1174-1181 Body_part denotes surface
T8 1407-1411 Body_part denotes cell
T9 1662-1666 Body_part denotes cell
T10 1667-1674 Body_part denotes surface
T11 1710-1716 Body_part denotes tissue
T12 1754-1780 Body_part denotes reticuloendothelial system

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 1570-1579 Glycan_Motif denotes galactose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G68158BT|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G65889KE

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 1570-1579 https://glytoucan.org/Structures/Glycans/G65889KE denotes galactose
T2 1570-1579 https://glytoucan.org/Structures/Glycans/G68158BT denotes galactose

sentences

Id Subject Object Predicate Lexical cue
T1 0-91 Sentence denotes Functional analysis of the putative integrin recognition motif on adeno-associated virus 9.
T2 92-183 Sentence denotes Adeno-associated viruses (AAVs) display a highly conserved NGR motif on the capsid surface.
T3 184-342 Sentence denotes Earlier studies have established this tripeptide motif as being essential for integrin-mediated uptake of recombinant AAV serotype 2 (AAV2) in cultured cells.
T4 343-509 Sentence denotes However, functional attributes of this putative integrin recognition motif in other recombinant AAV serotypes displaying systemic transduction in vivo remain unknown.
T5 510-628 Sentence denotes In this study, we dissect the biology of an integrin domain capsid mutant derived from the human isolate AAV9 in mice.
T6 629-695 Sentence denotes The AAV9/NGA mutant shows decreased systemic transduction in mice.
T7 696-845 Sentence denotes This defective phenotype was accompanied by rapid clearance of mutant virions from the blood circulation and nonspecific sequestration by the spleen.
T8 846-978 Sentence denotes Transient vascular hyperpermeability, induced by histamine coinjection, exacerbated AAV9/NGA uptake by the spleen but not the liver.
T9 979-1115 Sentence denotes However, such treatment did not affect AAV9 virions, suggesting a potential entry/post-entry defect for the mutant in different tissues.
T10 1116-1259 Sentence denotes Further characterization revealed modestly decreased cell surface binding but a more pronounced defect in the cellular entry of mutant virions.
T11 1260-1453 Sentence denotes These findings were corroborated by the observation that blocking multiple integrins adversely affected recombinant AAV9 transduction in different cell types, albeit with variable efficiencies.
T12 1454-1781 Sentence denotes From a structural perspective, we observed that the integrin recognition motif is located in close proximity to the galactose binding footprint on AAV9 capsids and postulate that this feature could influence cell surface attachment, cellular uptake at the tissue level, and systemic clearance by the reticuloendothelial system.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 783-788 http://purl.obolibrary.org/obo/MAT_0000083 denotes blood
T2 783-788 http://purl.obolibrary.org/obo/MAT_0000315 denotes blood
T3 838-844 http://purl.obolibrary.org/obo/MAT_0000085 denotes spleen
T4 953-959 http://purl.obolibrary.org/obo/MAT_0000085 denotes spleen
T5 972-977 http://purl.obolibrary.org/obo/MAT_0000097 denotes liver
T6 1553-1562 http://purl.obolibrary.org/obo/MAT_0000491 denotes proximity

GlyCosmos6-CLO

Id Subject Object Predicate Lexical cue
T1 327-341 http://purl.obolibrary.org/obo/CL_0000010 denotes cultured cells
T2 336-341 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3 1169-1173 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T4 1407-1417 http://purl.obolibrary.org/obo/CL_0000000 denotes cell types
T5 1407-1411 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T6 1662-1666 http://purl.obolibrary.org/obo/GO_0005623 denotes cell

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 66-90 OrganismTaxon denotes adeno-associated virus 9 NCBItxid:235455
T2 92-116 OrganismTaxon denotes Adeno-associated viruses NCBItxid:272636
T3 318-322 OrganismTaxon denotes AAV2 NCBItxid:10804
T4 601-606 OrganismTaxon denotes human NCBItxid:9606
T5 623-627 OrganismTaxon denotes mice NCBItxid:10088|NCBItxid:10095
T7 690-694 OrganismTaxon denotes mice NCBItxid:10088|NCBItxid:10095