PubMed:2535496 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/2535496","sourcedb":"PubMed","sourceid":"2535496","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/2535496","text":"Structure of the major O-glycosidic oligosaccharide of monkey erythrocyte glycophorin.\nSialic acids and the major O-glycosidic oligosaccharide of glycophorin MK from monkey (Japanese monkey, Macaca fuscata) erythrocyte membranes were characterized. N-Glycolylneuraminic acid (Neu5Gc) was found as the major sialic acid, which was confirmed by gas-liquid chromatography-mass spectrometry as the trimethylsilyl methyl ester. Three O-glycosidic oligosaccharide units were obtained from a tryptic glycopeptide that contained all of the carbohydrate units in glycophorin MK by mild alkaline borohydride/borotritide treatment. Carbohydrate analyses of the oligosaccharides revealed that they were composed of Neu5Gc, galactose and N-acetylgalactosaminitol in the molar ratios of 1:1:1 (trisaccharide), 2:1:1 (tetrasaccharide) and 3:1:1 (pentasaccharide). The content of oligosaccharide units was estimated to be 1:12:5 for penta-, tetra- and trisaccharide, respectively, based on the yields, the molecular weight, and the number of oligosaccharide attachment sites in the amino-acid sequence. The tetrasaccharide was the major oligosaccharide and its structure was proposed to be Neu5Gc alpha 2-3Gal beta 1-3[Neu5Gc alpha 2-6]GalNAcol.","tracks":[{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":207,"end":218},"obj":"HP_0001901"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"}]},{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":86},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":87,"end":248},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":249,"end":422},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":423,"end":620},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":621,"end":848},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":849,"end":1086},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":1087,"end":1229},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":86},"obj":"Sentence"},{"id":"T2","span":{"begin":87,"end":248},"obj":"Sentence"},{"id":"T3","span":{"begin":249,"end":422},"obj":"Sentence"},{"id":"T4","span":{"begin":423,"end":620},"obj":"Sentence"},{"id":"T5","span":{"begin":621,"end":848},"obj":"Sentence"},{"id":"T6","span":{"begin":849,"end":1086},"obj":"Sentence"},{"id":"T7","span":{"begin":1087,"end":1229},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":55,"end":61},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":166,"end":172},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":174,"end":189},"obj":"OrganismTaxon"},{"id":"T6","span":{"begin":191,"end":205},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9479"},{"id":"A2","pred":"db_id","subj":"T1","obj":"9527"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9479"},{"id":"A4","pred":"db_id","subj":"T3","obj":"9527"},{"id":"A5","pred":"db_id","subj":"T5","obj":"9542"},{"id":"A6","pred":"db_id","subj":"T6","obj":"9542"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T3","pred":"source","obj":"NCBITAXON"},{"subj":"T5","pred":"source","obj":"NCBITAXON"},{"subj":"T6","pred":"source","obj":"NCBITAXON"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":87,"end":99},"obj":"https://glytoucan.org/Structures/Glycans/G81533KY"},{"id":"T2","span":{"begin":307,"end":318},"obj":"https://glytoucan.org/Structures/Glycans/G81533KY"},{"id":"T3","span":{"begin":711,"end":720},"obj":"https://glytoucan.org/Structures/Glycans/G65889KE"},{"id":"T4","span":{"begin":711,"end":720},"obj":"https://glytoucan.org/Structures/Glycans/G68158BT"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T4","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":87,"end":99},"obj":"Glycan_Motif"},{"id":"T2","span":{"begin":307,"end":318},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":711,"end":720},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY"},{"id":"A2","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G68158BT"},{"id":"A4","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G65889KE"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":62,"end":73},"obj":"Body_part"},{"id":"T2","span":{"begin":207,"end":218},"obj":"Body_part"},{"id":"T3","span":{"begin":219,"end":228},"obj":"Body_part"},{"id":"T6","span":{"begin":757,"end":762},"obj":"Body_part"},{"id":"T7","span":{"begin":1042,"end":1058},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL_0000232"},{"id"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