PubMed:25286189
Annnotations
Goldhamster2_Cellosaurus
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 221-222 | CVCL_6479|Finite_cell_line|Mus musculus | denotes | a |
T2 | 303-304 | CVCL_6479|Finite_cell_line|Mus musculus | denotes | a |
T3 | 408-409 | CVCL_6479|Finite_cell_line|Mus musculus | denotes | a |
T4 | 462-463 | CVCL_6479|Finite_cell_line|Mus musculus | denotes | a |
T5 | 579-580 | CVCL_6479|Finite_cell_line|Mus musculus | denotes | a |
T6 | 803-804 | CVCL_6479|Finite_cell_line|Mus musculus | denotes | a |
T7 | 820-821 | CVCL_6479|Finite_cell_line|Mus musculus | denotes | a |
T8 | 856-858 | CVCL_8754|Cancer_cell_line|Homo sapiens | denotes | an |
T9 | 856-858 | CVCL_H241|Cancer_cell_line|Homo sapiens | denotes | an |
T10 | 999-1000 | CVCL_6479|Finite_cell_line|Mus musculus | denotes | a |
T11 | 1726-1728 | CVCL_5M23|Cancer_cell_line|Mesocricetus auratus | denotes | We |
T12 | 1743-1744 | CVCL_6479|Finite_cell_line|Mus musculus | denotes | a |
T13 | 1821-1823 | CVCL_8754|Cancer_cell_line|Homo sapiens | denotes | an |
T14 | 1821-1823 | CVCL_H241|Cancer_cell_line|Homo sapiens | denotes | an |
GoldHamster
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 19-29 | 2294143 | denotes | Drosophila |
T2 | 19-29 | 504603 | denotes | Drosophila |
T3 | 19-29 | 2294139 | denotes | Drosophila |
T4 | 19-29 | 2294145 | denotes | Drosophila |
T5 | 19-29 | 2294141 | denotes | Drosophila |
T6 | 19-29 | 2294142 | denotes | Drosophila |
T7 | 19-29 | 2294144 | denotes | Drosophila |
T8 | 19-29 | 2294140 | denotes | Drosophila |
T9 | 19-29 | D004330 | denotes | Drosophila |
T10 | 19-29 | 2080301 | denotes | Drosophila |
T11 | 36-42 | UBERON:0000473 | denotes | testis |
T12 | 54-58 | UBERON:0003101 | denotes | male |
T13 | 54-58 | CHEBI:30780 | denotes | male |
T14 | 69-74 | D003560 | denotes | cysts |
T15 | 69-74 | D003560 | denotes | cysts |
T16 | 135-150 | GO:0007283 | denotes | spermatogenesis |
T27 | 169-192 | D004331 | denotes | Drosophila melanogaster |
T28 | 169-192 | 7227 | denotes | Drosophila melanogaster |
T29 | 194-198 | UBERON:0003101 | denotes | male |
T30 | 194-198 | CHEBI:30780 | denotes | male |
T31 | 199-209 | UBERON:3010292 | denotes | germ cells |
T32 | 257-266 | UBERON:0004529 | denotes | processes |
T33 | 305-314 | CL:0000034 | denotes | stem cell |
T34 | 354-361 | GO:0051321 | denotes | meiosis |
T35 | 389-399 | UBERON:3010292 | denotes | germ cells |
T37 | 515-524 | GO:0000785 | denotes | chromatin |
T36 | 515-524 | D002843 | denotes | chromatin |
T39 | 529-536 | PR:000041244 | denotes | histone |
T38 | 529-536 | D006657 | denotes | histone |
T40 | 529-536 | CHEBI:15358 | denotes | histone |
T41 | 540-549 | P0C230 | denotes | protamine |
T43 | 540-549 | P15340 | denotes | protamine |
T44 | 540-549 | P29629 | denotes | protamine |
T45 | 540-549 | Q91185 | denotes | protamine |
T46 | 540-549 | P14402 | denotes | protamine |
T47 | 540-549 | P17502 | denotes | protamine |
T48 | 540-549 | Q9PS27 | denotes | protamine |
T50 | 540-549 | P69014 | denotes | protamine |
T49 | 540-549 | CHEBI:16757 | denotes | protamine |
T42 | 540-549 | 8826 | denotes | protamine |
T55 | 581-588 | SO:0000104 | denotes | protein |
T54 | 581-588 | PR:000000001 | denotes | protein |
T51 | 581-588 | GO:0003675 | denotes | protein |
T52 | 581-588 | CHEBI:36080 | denotes | protein |
T53 | 581-588 | CHEBI:11122 | denotes | protein |
T56 | 605-620 | GO:0007283 | denotes | spermatogenesis |
T58 | 627-638 | SO:0001148 | denotes | mutagenesis |
T57 | 627-638 | GO:0006280 | denotes | mutagenesis |
T59 | 692-701 | P02301 | denotes | embryonic |
T60 | 692-701 | PR:000029532 | denotes | embryonic |
T63 | 703-706 | SO:0002045 | denotes | pre |
T62 | 703-706 | PR:Q8LLD0 | denotes | pre |
T64 | 703-706 | UBERON:0001782 | denotes | pre |
T61 | 703-706 | GO:0003904 | denotes | pre |
T69 | 744-751 | SO:0000104 | denotes | protein |
T68 | 744-751 | PR:000000001 | denotes | protein |
T65 | 744-751 | GO:0003675 | denotes | protein |
T66 | 744-751 | CHEBI:36080 | denotes | protein |
T67 | 744-751 | CHEBI:11122 | denotes | protein |
T73 | 822-829 | PR:000000001 | denotes | protein |
T71 | 822-829 | CHEBI:36080 | denotes | protein |
T72 | 822-829 | CHEBI:11122 | denotes | protein |
T74 | 822-829 | SO:0000104 | denotes | protein |
T70 | 822-829 | GO:0003675 | denotes | protein |
T75 | 959-967 | UBERON:0000468 | denotes | organism |
T86 | 968-991 | D004331 | denotes | Drosophila melanogaster |
T87 | 968-991 | 7227 | denotes | Drosophila melanogaster |
T88 | 1032-1038 | UBERON:0000473 | denotes | testes |
T89 | 1048-1053 | D003560 | denotes | cysts |
T90 | 1048-1053 | D003560 | denotes | cysts |
T91 | 1057-1067 | UBERON:3010292 | denotes | germ cells |
T92 | 1089-1094 | D011679 | denotes | pupae |
T93 | 1089-1094 | UBERON:0003143 | denotes | pupae |
T94 | 1207-1217 | UBERON:3010292 | denotes | germ cells |
T95 | 1221-1227 | UBERON:0000473 | denotes | testes |
T96 | 1245-1250 | D003560 | denotes | cysts |
T97 | 1245-1250 | D003560 | denotes | cysts |
T98 | 1280-1286 | UBERON:0000473 | denotes | testes |
T99 | 1291-1301 | UBERON:3010292 | denotes | germ cells |
T100 | 1344-1354 | CHEBI:35222 | denotes | inhibitors |
T101 | 1391-1400 | SO:0001185 | denotes | enzymatic |
T102 | 1418-1433 | GO:0007283 | denotes | spermatogenesis |
T103 | 1534-1540 | UBERON:0000473 | denotes | testes |
T104 | 1555-1560 | D003560 | denotes | cysts |
T105 | 1555-1560 | D003560 | denotes | cysts |
T106 | 1602-1608 | UBERON:0000473 | denotes | testes |
T107 | 1687-1693 | UBERON:0000473 | denotes | testes |
T108 | 1708-1713 | D003560 | denotes | cysts |
T109 | 1708-1713 | D003560 | denotes | cysts |
T110 | 1789-1804 | GO:0007283 | denotes | spermatogenesis |
T112 | 1844-1851 | PR:000041244 | denotes | histone |
T111 | 1844-1851 | D006657 | denotes | histone |
T113 | 1844-1851 | CHEBI:15358 | denotes | histone |
T114 | 1855-1864 | P0C230 | denotes | protamine |
T116 | 1855-1864 | P15340 | denotes | protamine |
T117 | 1855-1864 | P29629 | denotes | protamine |
T118 | 1855-1864 | Q91185 | denotes | protamine |
T119 | 1855-1864 | P14402 | denotes | protamine |
T120 | 1855-1864 | P17502 | denotes | protamine |
T121 | 1855-1864 | Q9PS27 | denotes | protamine |
T123 | 1855-1864 | P69014 | denotes | protamine |
T115 | 1855-1864 | 8826 | denotes | protamine |
T122 | 1855-1864 | CHEBI:16757 | denotes | protamine |
T128 | 1882-1907 | P9WKY4 | denotes | histone acetyltransferase |
T129 | 1882-1907 | P9WKY5 | denotes | histone acetyltransferase |
T130 | 1882-1917 | CHEBI:76395 | denotes | histone acetyltransferase inhibitor |
T134 | 1918-1932 | C088115 | denotes | anacardic acid |
T135 | 1918-1932 | CHEBI:2696 | denotes | anacardic acid |
T136 | 1918-1932 | C088115 | denotes | anacardic acid |
T139 | 2033-2036 | PR:Q8LLD0 | denotes | pre |
T141 | 2033-2036 | UBERON:0001782 | denotes | pre |
T140 | 2033-2036 | SO:0002045 | denotes | pre |
T138 | 2033-2036 | GO:0003904 | denotes | pre |
T142 | 2055-2070 | GO:0007283 | denotes | spermatogenesis |
PubMed_ArguminSci
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 128-267 | DRI_Background | denotes | During spermatogenesis in mammals and in Drosophila melanogaster, male germ cells develop in a series of essential developmental processes. |
T2 | 268-362 | DRI_Approach | denotes | This includes differentiation from a stem cell population, mitotic amplification, and meiosis. |
T3 | 363-514 | DRI_Background | denotes | In addition, post-meiotic germ cells undergo a dramatic morphological reshaping process as well as a global epigenetic reconfiguration of the germ line |
T4 | 550-557 | DRI_Background | denotes | switch. |
T5 | 558-772 | DRI_Background | denotes | Studying the role of a protein in post-meiotic spermatogenesis using mutagenesis or other genetic tools is often impeded by essential embryonic, pre-meiotic, or meiotic functions of the protein under investigation. |
T6 | 773-948 | DRI_Challenge | denotes | The post-meiotic phenotype of a mutant of such a protein could be obscured through an earlier developmental block, or the interpretation of the phenotype could be complicated. |
T7 | 949-1141 | DRI_Challenge | denotes | The model organism Drosophila melanogaster offers a bypass to this problem: intact testes and even cysts of germ cells dissected from early pupae are able to develop ex vivo in culture medium. |
T8 | 1142-1251 | DRI_Background | denotes | Making use of such cultures allows microscopic imaging of living germ cells in testes and of germ-line cysts. |
T9 | 1252-1465 | DRI_Background | denotes | Importantly, the cultivated testes and germ cells also become accessible to pharmacological inhibitors, thereby permitting manipulation of enzymatic functions during spermatogenesis, including post-meiotic stages. |
T10 | 1466-1561 | DRI_Outcome | denotes | The protocol presented describes how to dissect and cultivate pupal testes and germ-line cysts. |
T11 | 1562-1725 | DRI_Background | denotes | Information on the development of pupal testes and culture conditions are provided alongside microscope imaging data of live testes and germ-line cysts in culture. |
T12 | 1726-1933 | DRI_Background | denotes | We also describe a pharmacological assay to study post-meiotic spermatogenesis, exemplified by an assay targeting the histone-to-protamine switch using the histone acetyltransferase inhibitor anacardic acid. |
T13 | 1934-2071 | DRI_Background | denotes | In principle, this cultivation method could be adapted to address many other research questions in pre- and post-meiotic spermatogenesis. |