PubMed:2525070 JSONTXT

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":204},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":205,"end":424},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":425,"end":516},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":517,"end":763},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":764,"end":901},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":902,"end":995},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":996,"end":1084},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1085,"end":1428},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1429,"end":1530},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":204},"obj":"Sentence"},{"id":"T2","span":{"begin":205,"end":424},"obj":"Sentence"},{"id":"T3","span":{"begin":425,"end":516},"obj":"Sentence"},{"id":"T4","span":{"begin":517,"end":763},"obj":"Sentence"},{"id":"T5","span":{"begin":764,"end":901},"obj":"Sentence"},{"id":"T6","span":{"begin":902,"end":995},"obj":"Sentence"},{"id":"T7","span":{"begin":996,"end":1084},"obj":"Sentence"},{"id":"T8","span":{"begin":1085,"end":1428},"obj":"Sentence"},{"id":"T9","span":{"begin":1429,"end":1530},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Use of 13C-n.m.r. spectroscopy for the quantitative estimation of 3-O- and 3,6-di-O-substituted D-glucopyranosyl residues in alpha-D-glucans formed by the D-glucosyltransferases of Streptococcus sobrinus.\nThe 13C-n.m.r. spectra of the three alpha-D-glucans from Streptococcus sobrinus and the dextran from Leuconostoc mesenteroides, which differ widely in the ratios of omega (terminal, nonreducing) D-glucopyranosyl groups: 3-:6-:3,6-linked D-glucopyranosyl (Glc) residues, were measured in 0.5M NaOH at 22 degrees. The C-1 signals of 3-O-substituted Glc in a linear sequence, 6-O-substituted Glc in a linear sequence, 3,6-di-O-substituted Glc in a (1----6)-linked sequence, and Glc attached to O-3 of 3,6-di-O-substituted Glc were distinguished from each other. The C-3 signal of 3,6-linked Glc appeared downfield by 0.6 to 1.0 p.p.m. compared to the C-3 signal of 3-linked Glc in a linear sequence. The C-6 signals of omega-terminal, 3-linked, 6-linked, and 3,6-linked Glc were also assigned. The C-2 signal of 3-linked Glc in a linear sequence appeared separately, at 73.76 p.p.m. Based on these assignments, the various D-glucopyranosyl residues of the S. sobrinus alpha-D-glucans were quantitatively estimated from the signal areas of the C-2 atom of 3-linked Glc, the C-3 atom of 3-linked and 3,6-linked Glc, the C-6 atom of 6-linked and 3,6-linked Glc, and the C-6 atom of the omega-Glc groups and 3-linked Glc residues. The figures thus derived for the linkage ratios were close to those obtained by methylation analysis."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":181,"end":203},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":262,"end":284},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":306,"end":331},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"1310"},{"id":"A2","pred":"db_id","subj":"T2","obj":"1310"},{"id":"A3","pred":"db_id","subj":"T3","obj":"1245"}],"text":"Use of 13C-n.m.r. spectroscopy for the quantitative estimation of 3-O- and 3,6-di-O-substituted D-glucopyranosyl residues in alpha-D-glucans formed by the D-glucosyltransferases of Streptococcus sobrinus.\nThe 13C-n.m.r. spectra of the three alpha-D-glucans from Streptococcus sobrinus and the dextran from Leuconostoc mesenteroides, which differ widely in the ratios of omega (terminal, nonreducing) D-glucopyranosyl groups: 3-:6-:3,6-linked D-glucopyranosyl (Glc) residues, were measured in 0.5M NaOH at 22 degrees. The C-1 signals of 3-O-substituted Glc in a linear sequence, 6-O-substituted Glc in a linear sequence, 3,6-di-O-substituted Glc in a (1----6)-linked sequence, and Glc attached to O-3 of 3,6-di-O-substituted Glc were distinguished from each other. The C-3 signal of 3,6-linked Glc appeared downfield by 0.6 to 1.0 p.p.m. compared to the C-3 signal of 3-linked Glc in a linear sequence. The C-6 signals of omega-terminal, 3-linked, 6-linked, and 3,6-linked Glc were also assigned. The C-2 signal of 3-linked Glc in a linear sequence appeared separately, at 73.76 p.p.m. Based on these assignments, the various D-glucopyranosyl residues of the S. sobrinus alpha-D-glucans were quantitatively estimated from the signal areas of the C-2 atom of 3-linked Glc, the C-3 atom of 3-linked and 3,6-linked Glc, the C-6 atom of 6-linked and 3,6-linked Glc, and the C-6 atom of the omega-Glc groups and 3-linked Glc residues. The figures thus derived for the linkage ratios were close to those obtained by methylation analysis."}