> top > docs > PubMed:25095703 > annotations

PubMed:25095703 JSONTXT

Annnotations TAB JSON ListView MergeView

OryzaGP_2021

Id Subject Object Predicate Lexical cue
T1 563-565 http://identifiers.org/oryzabase.gene/7498 denotes CS
T2 563-565 http://identifiers.org/oryzabase.gene/16772 denotes CS
T3 747-749 http://identifiers.org/oryzabase.gene/7498 denotes CS
T4 747-749 http://identifiers.org/oryzabase.gene/16772 denotes CS
T5 1102-1104 http://identifiers.org/oryzabase.gene/7498 denotes CS
T6 1102-1104 http://identifiers.org/oryzabase.gene/16772 denotes CS
T7 1285-1314 http://identifiers.org/oryzabase.gene/744 denotes granule-bound starch synthase
T8 1316-1321 http://identifiers.org/oryzabase.gene/744 denotes GBSSI
T9 1442-1444 http://identifiers.org/oryzabase.gene/7498 denotes CS
T10 1442-1444 http://identifiers.org/oryzabase.gene/16772 denotes CS
T11 1459-1464 http://identifiers.org/oryzabase.gene/744 denotes GBSSI
T12 1561-1563 http://identifiers.org/oryzabase.gene/7498 denotes CS
T13 1561-1563 http://identifiers.org/oryzabase.gene/16772 denotes CS
T14 1639-1644 http://identifiers.org/oryzabase.gene/744 denotes GBSSI
T15 1864-1869 http://identifiers.org/oryzabase.gene/744 denotes GBSSI
T16 1921-1923 http://identifiers.org/oryzabase.gene/7498 denotes CS
T17 1921-1923 http://identifiers.org/oryzabase.gene/16772 denotes CS
T18 2066-2071 http://identifiers.org/oryzabase.gene/744 denotes GBSSI
T48516 563-565 http://identifiers.org/ricegap/LOC_Os05g28200 denotes CS
T92536 563-565 http://identifiers.org/ricegap/LOC_Os03g14990 denotes CS
T90122 747-749 http://identifiers.org/ricegap/LOC_Os05g28200 denotes CS
T11283 747-749 http://identifiers.org/ricegap/LOC_Os03g14990 denotes CS
T59203 1102-1104 http://identifiers.org/ricegap/LOC_Os05g28200 denotes CS
T34915 1102-1104 http://identifiers.org/ricegap/LOC_Os03g14990 denotes CS
T27264 1285-1314 http://identifiers.org/ricegap/LOC_Os06g04200 denotes granule-bound starch synthase
T85352 1316-1321 http://identifiers.org/ricegap/LOC_Os06g04200 denotes GBSSI
T25743 1442-1444 http://identifiers.org/ricegap/LOC_Os05g28200 denotes CS
T95669 1442-1444 http://identifiers.org/ricegap/LOC_Os03g14990 denotes CS
T83391 1459-1464 http://identifiers.org/ricegap/LOC_Os06g04200 denotes GBSSI
T20182 1561-1563 http://identifiers.org/ricegap/LOC_Os05g28200 denotes CS
T52220 1561-1563 http://identifiers.org/ricegap/LOC_Os03g14990 denotes CS
T78048 1639-1644 http://identifiers.org/ricegap/LOC_Os06g04200 denotes GBSSI
T90628 1864-1869 http://identifiers.org/ricegap/LOC_Os06g04200 denotes GBSSI
T51075 1921-1923 http://identifiers.org/ricegap/LOC_Os05g28200 denotes CS
T43042 1921-1923 http://identifiers.org/ricegap/LOC_Os03g14990 denotes CS
T96384 2066-2071 http://identifiers.org/ricegap/LOC_Os06g04200 denotes GBSSI
T39946 563-565 http://identifiers.org/rapdb.locus/Os05g0349700 denotes CS
T11509 563-565 http://identifiers.org/rapdb.locus/Os03g0254800 denotes CS
T84038 747-749 http://identifiers.org/rapdb.locus/Os05g0349700 denotes CS
T6817 747-749 http://identifiers.org/rapdb.locus/Os03g0254800 denotes CS
T97991 1102-1104 http://identifiers.org/rapdb.locus/Os05g0349700 denotes CS
T73733 1102-1104 http://identifiers.org/rapdb.locus/Os03g0254800 denotes CS
T23781 1285-1314 http://identifiers.org/rapdb.locus/Os06g0133000 denotes granule-bound starch synthase
T43759 1316-1321 http://identifiers.org/rapdb.locus/Os06g0133000 denotes GBSSI
T88549 1442-1444 http://identifiers.org/rapdb.locus/Os05g0349700 denotes CS
T4376 1442-1444 http://identifiers.org/rapdb.locus/Os03g0254800 denotes CS
T21102 1459-1464 http://identifiers.org/rapdb.locus/Os06g0133000 denotes GBSSI
T20773 1561-1563 http://identifiers.org/rapdb.locus/Os05g0349700 denotes CS
T57749 1561-1563 http://identifiers.org/rapdb.locus/Os03g0254800 denotes CS
T44817 1639-1644 http://identifiers.org/rapdb.locus/Os06g0133000 denotes GBSSI
T30466 1864-1869 http://identifiers.org/rapdb.locus/Os06g0133000 denotes GBSSI
T11870 1921-1923 http://identifiers.org/rapdb.locus/Os05g0349700 denotes CS
T37557 1921-1923 http://identifiers.org/rapdb.locus/Os03g0254800 denotes CS
T15274 2066-2071 http://identifiers.org/rapdb.locus/Os06g0133000 denotes GBSSI
T35573 563-565 http://identifiers.org/uniprot/Q5W6H5 denotes CS
T4255 563-565 http://identifiers.org/uniprot/B8AKA5 denotes CS
T9720 563-565 http://identifiers.org/uniprot/B7ENB3 denotes CS
T54946 563-565 http://identifiers.org/uniprot/A4GUA1 denotes CS
T6090 747-749 http://identifiers.org/uniprot/Q5W6H5 denotes CS
T57450 747-749 http://identifiers.org/uniprot/B8AKA5 denotes CS
T16941 747-749 http://identifiers.org/uniprot/B7ENB3 denotes CS
T38283 747-749 http://identifiers.org/uniprot/A4GUA1 denotes CS
T59077 1102-1104 http://identifiers.org/uniprot/Q5W6H5 denotes CS
T61099 1102-1104 http://identifiers.org/uniprot/B8AKA5 denotes CS
T39763 1102-1104 http://identifiers.org/uniprot/B7ENB3 denotes CS
T6471 1102-1104 http://identifiers.org/uniprot/A4GUA1 denotes CS
T46281 1285-1314 http://identifiers.org/uniprot/Q0DEV5 denotes granule-bound starch synthase
T32528 1316-1321 http://identifiers.org/uniprot/Q0DEV5 denotes GBSSI
T17521 1442-1444 http://identifiers.org/uniprot/Q5W6H5 denotes CS
T68912 1442-1444 http://identifiers.org/uniprot/B8AKA5 denotes CS
T11033 1442-1444 http://identifiers.org/uniprot/B7ENB3 denotes CS
T1171 1442-1444 http://identifiers.org/uniprot/A4GUA1 denotes CS
T19 1459-1464 http://identifiers.org/uniprot/Q0DEV5 denotes GBSSI
T20 1561-1563 http://identifiers.org/uniprot/Q5W6H5 denotes CS
T21 1561-1563 http://identifiers.org/uniprot/B8AKA5 denotes CS
T22 1561-1563 http://identifiers.org/uniprot/B7ENB3 denotes CS
T23 1561-1563 http://identifiers.org/uniprot/A4GUA1 denotes CS
T24 1639-1644 http://identifiers.org/uniprot/Q0DEV5 denotes GBSSI
T25 1864-1869 http://identifiers.org/uniprot/Q0DEV5 denotes GBSSI
T26 1921-1923 http://identifiers.org/uniprot/Q5W6H5 denotes CS
T27 1921-1923 http://identifiers.org/uniprot/B8AKA5 denotes CS
T28 1921-1923 http://identifiers.org/uniprot/B7ENB3 denotes CS
T29 1921-1923 http://identifiers.org/uniprot/A4GUA1 denotes CS
T30 2066-2071 http://identifiers.org/uniprot/Q0DEV5 denotes GBSSI
M_0 1505-1513 hunflair:NA:Chemical denotes tyrosine
M_1 132-137 hunflair:NA:Species denotes wheat
M_2 540-545 hunflair:NA:Species denotes wheat
M_3 872-877 hunflair:NA:Species denotes wheat
M_4 648-652 hunflair:NA:CellLine denotes Bd21
M_5 754-758 hunflair:NA:CellLine denotes Bd21
M_6 806-810 hunflair:NA:CellLine denotes Bd21
M_7 925-929 hunflair:NA:CellLine denotes Bd21
M_8 1429-1433 hunflair:NA:CellLine denotes Bd21
M_9 1622-1626 hunflair:NA:CellLine denotes Bd21
M_10 1710-1714 hunflair:NA:CellLine denotes Bd21
M_11 1932-1936 hunflair:NA:CellLine denotes Bd21
M_12 2060-2064 hunflair:NA:CellLine denotes Bd21
M_13 635-652 hunflair:NA:Species denotes Brachypodium Bd21
M_14 1091-1098 hunflair:NA:Gene denotes enzymes
M_15 1316-1321 hunflair:NA:Gene denotes GBSSI
M_16 1459-1464 hunflair:NA:Gene denotes GBSSI
M_17 1639-1644 hunflair:NA:Gene denotes GBSSI
M_18 1864-1869 hunflair:NA:Gene denotes GBSSI
M_19 2066-2071 hunflair:NA:Gene denotes GBSSI
M_20 1976-1982 hunflair:NA:Chemical denotes Tyr185
M_21 1998-2006 hunflair:NA:Species denotes Triticum
M_22 151-160 hunflair:NA:Species denotes peregrina
M_23 580-589 hunflair:NA:Species denotes peregrina
M_24 1113-1122 hunflair:NA:Species denotes peregrina
M_25 1572-1581 hunflair:NA:Species denotes peregrina
M_26 84-107 hunflair:NA:Species denotes Brachypodium distachyon
M_27 459-482 hunflair:NA:Species denotes Brachypodium distachyon
M_28 886-890 hunflair:NA:Species denotes rice
M_29 1487-1496 hunflair:NA:Chemical denotes threonine
M_30 142-150 hunflair:NA:Species denotes Aegilops
M_31 571-579 hunflair:NA:Species denotes Aegilops
M_32 2011-2019 hunflair:NA:Species denotes Aegilops
M_33 142-160 hunflair:NA:Species denotes Aegilops peregrina
M_34 571-589 hunflair:NA:Species denotes Aegilops peregrina
M_35 1109-1112 hunflair:NA:Species denotes Ae.
M_36 1568-1571 hunflair:NA:Species denotes Ae.
M_37 1965-1971 hunflair:NA:Chemical denotes Thr183
M_38 1299-1314 hunflair:NA:Gene denotes starch synthase
M_39 1525-1540 hunflair:NA:Gene denotes starch synthase

OryzaGP_2021_v2

Id Subject Object Predicate Lexical cue
T1 563-565 http://identifiers.org/oryzabase.gene/7498 denotes CS
T2 563-565 http://identifiers.org/oryzabase.gene/16772 denotes CS
T3 747-749 http://identifiers.org/oryzabase.gene/7498 denotes CS
T4 747-749 http://identifiers.org/oryzabase.gene/16772 denotes CS
T5 1102-1104 http://identifiers.org/oryzabase.gene/7498 denotes CS
T6 1102-1104 http://identifiers.org/oryzabase.gene/16772 denotes CS
T7 1316-1321 http://identifiers.org/oryzabase.gene/744 denotes GBSSI
T8 1442-1444 http://identifiers.org/oryzabase.gene/7498 denotes CS
T9 1442-1444 http://identifiers.org/oryzabase.gene/16772 denotes CS
T10 1459-1464 http://identifiers.org/oryzabase.gene/744 denotes GBSSI
T11 1561-1563 http://identifiers.org/oryzabase.gene/7498 denotes CS
T12 1561-1563 http://identifiers.org/oryzabase.gene/16772 denotes CS
T13 1639-1644 http://identifiers.org/oryzabase.gene/744 denotes GBSSI
T14 1864-1869 http://identifiers.org/oryzabase.gene/744 denotes GBSSI
T15 1921-1923 http://identifiers.org/oryzabase.gene/7498 denotes CS
T16 1921-1923 http://identifiers.org/oryzabase.gene/16772 denotes CS
T17 2066-2071 http://identifiers.org/oryzabase.gene/744 denotes GBSSI
T76112 563-565 http://identifiers.org/rapdb.locus/Os05g0349700 denotes CS
T98365 563-565 http://identifiers.org/rapdb.locus/Os03g0254800 denotes CS
T26553 747-749 http://identifiers.org/rapdb.locus/Os05g0349700 denotes CS
T85706 747-749 http://identifiers.org/rapdb.locus/Os03g0254800 denotes CS
T22262 1102-1104 http://identifiers.org/rapdb.locus/Os05g0349700 denotes CS
T41692 1102-1104 http://identifiers.org/rapdb.locus/Os03g0254800 denotes CS
T67279 1316-1321 http://identifiers.org/rapdb.locus/Os06g0133000 denotes GBSSI
T41463 1442-1444 http://identifiers.org/rapdb.locus/Os05g0349700 denotes CS
T72474 1442-1444 http://identifiers.org/rapdb.locus/Os03g0254800 denotes CS
T97257 1459-1464 http://identifiers.org/rapdb.locus/Os06g0133000 denotes GBSSI
T41709 1561-1563 http://identifiers.org/rapdb.locus/Os05g0349700 denotes CS
T3346 1561-1563 http://identifiers.org/rapdb.locus/Os03g0254800 denotes CS
T58506 1639-1644 http://identifiers.org/rapdb.locus/Os06g0133000 denotes GBSSI
T52218 1864-1869 http://identifiers.org/rapdb.locus/Os06g0133000 denotes GBSSI
T5697 1921-1923 http://identifiers.org/rapdb.locus/Os05g0349700 denotes CS
T22671 1921-1923 http://identifiers.org/rapdb.locus/Os03g0254800 denotes CS
T94427 2066-2071 http://identifiers.org/rapdb.locus/Os06g0133000 denotes GBSSI

OryzaGP_2021_FLAIR

Id Subject Object Predicate Lexical cue
M_0 1505-1513 hunflair:NA:Chemical denotes tyrosine
M_1 132-137 hunflair:NA:Species denotes wheat
M_2 540-545 hunflair:NA:Species denotes wheat
M_3 872-877 hunflair:NA:Species denotes wheat
M_4 648-652 hunflair:NA:CellLine denotes Bd21
M_5 754-758 hunflair:NA:CellLine denotes Bd21
M_6 806-810 hunflair:NA:CellLine denotes Bd21
M_7 925-929 hunflair:NA:CellLine denotes Bd21
M_8 1429-1433 hunflair:NA:CellLine denotes Bd21
M_9 1622-1626 hunflair:NA:CellLine denotes Bd21
M_10 1710-1714 hunflair:NA:CellLine denotes Bd21
M_11 1932-1936 hunflair:NA:CellLine denotes Bd21
M_12 2060-2064 hunflair:NA:CellLine denotes Bd21
M_13 635-652 hunflair:NA:Species denotes Brachypodium Bd21
M_14 1091-1098 hunflair:NA:Gene denotes enzymes
M_15 1316-1321 hunflair:NA:Gene denotes GBSSI
M_16 1459-1464 hunflair:NA:Gene denotes GBSSI
M_17 1639-1644 hunflair:NA:Gene denotes GBSSI
M_18 1864-1869 hunflair:NA:Gene denotes GBSSI
M_19 2066-2071 hunflair:NA:Gene denotes GBSSI
M_20 1976-1982 hunflair:NA:Chemical denotes Tyr185
M_21 1998-2006 hunflair:NA:Species denotes Triticum
M_22 151-160 hunflair:NA:Species denotes peregrina
M_23 580-589 hunflair:NA:Species denotes peregrina
M_24 1113-1122 hunflair:NA:Species denotes peregrina
M_25 1572-1581 hunflair:NA:Species denotes peregrina
M_26 84-107 hunflair:NA:Species denotes Brachypodium distachyon
M_27 459-482 hunflair:NA:Species denotes Brachypodium distachyon
M_28 886-890 hunflair:NA:Species denotes rice
M_29 1487-1496 hunflair:NA:Chemical denotes threonine
M_30 142-150 hunflair:NA:Species denotes Aegilops
M_31 571-579 hunflair:NA:Species denotes Aegilops
M_32 2011-2019 hunflair:NA:Species denotes Aegilops
M_33 142-160 hunflair:NA:Species denotes Aegilops peregrina
M_34 571-589 hunflair:NA:Species denotes Aegilops peregrina
M_35 1109-1112 hunflair:NA:Species denotes Ae.
M_36 1568-1571 hunflair:NA:Species denotes Ae.
M_37 1965-1971 hunflair:NA:Chemical denotes Thr183
M_38 1299-1314 hunflair:NA:Gene denotes starch synthase
M_39 1525-1540 hunflair:NA:Gene denotes starch synthase

OryzaGP

Id Subject Object Predicate Lexical cue
T1 2066-2071 gene denotes GBSSI

Oryza_sentences

Id Subject Object Predicate Lexical cue
T1 0-161 Sentence denotes Dynamic development of starch granules and the regulation of starch biosynthesis in Brachypodium distachyon: comparison with common wheat and Aegilops peregrina.
T2 162-173 Sentence denotes BACKGROUND:
T3 174-324 Sentence denotes Thorough understanding of seed starch biosynthesis and accumulation mechanisms is of great importance for agriculture and crop improvement strategies.
T4 325-590 Sentence denotes We conducted the first comprehensive study of the dynamic development of starch granules and the regulation of starch biosynthesis in Brachypodium distachyon and compared the findings with those reported for common wheat (Chinese Spring, CS) and Aegilops peregrina.
T5 591-599 Sentence denotes RESULTS:
T6 600-759 Sentence denotes Only B-granules were identified in Brachypodium Bd21, and the shape variation and development of starch granules were similar in the B-granules of CS and Bd21.
T7 760-891 Sentence denotes Phylogenetic analysis showed that most of the Bd21 starch synthesis-related genes were more similar to those in wheat than in rice.
T8 892-1071 Sentence denotes Early expression of key genes in Bd21 starch biosynthesis mediate starch synthesis in the pericarp; intermediate-stage expression increases the number and size of starch granules.
T9 1072-1252 Sentence denotes In contrast, these enzymes in CS and Ae. peregrina were mostly expressed at intermediate stages, driving production of new B-granules and increasing the granule size, respectively.
T10 1253-1445 Sentence denotes Immunogold labeling showed that granule-bound starch synthase (GBSSI; related to amylose synthesis) was mainly present in starch granules: at lower levels in the B-granules of Bd21 than in CS.
T11 1446-1696 Sentence denotes Furthermore, GBSSI was phosphorylated at threonine 183 and tyrosine 185 in the starch synthase catalytic domain in CS and Ae. peregrina, but neither site was phosphorylated in Bd21, suggesting GBSSI phosphorylation could improve amylose biosynthesis.
T12 1697-1709 Sentence denotes CONCLUSIONS:
T13 1710-1863 Sentence denotes Bd21 contains only B-granules, and the expression of key genes in the three studied genera is consistent with the dynamic development of starch granules.
T14 1864-2065 Sentence denotes GBSSI is present in greater amounts in the B-granules of CS than in Bd21; two phosphorylation sites (Thr183 and Tyr185) were found in Triticum and Aegilops; these sites were not phosphorylated in Bd21.
T15 2066-2136 Sentence denotes GBSSI phosphorylation may reflect its importance in amylose synthesis.

Oryza_sentences

Id Subject Object Predicate Lexical cue
T1 0-161 Sentence denotes Dynamic development of starch granules and the regulation of starch biosynthesis in Brachypodium distachyon: comparison with common wheat and Aegilops peregrina.
T2 162-173 Sentence denotes BACKGROUND:
T3 174-324 Sentence denotes Thorough understanding of seed starch biosynthesis and accumulation mechanisms is of great importance for agriculture and crop improvement strategies.
T4 325-590 Sentence denotes We conducted the first comprehensive study of the dynamic development of starch granules and the regulation of starch biosynthesis in Brachypodium distachyon and compared the findings with those reported for common wheat (Chinese Spring, CS) and Aegilops peregrina.
T5 591-599 Sentence denotes RESULTS:
T6 600-759 Sentence denotes Only B-granules were identified in Brachypodium Bd21, and the shape variation and development of starch granules were similar in the B-granules of CS and Bd21.
T7 760-891 Sentence denotes Phylogenetic analysis showed that most of the Bd21 starch synthesis-related genes were more similar to those in wheat than in rice.
T8 892-1071 Sentence denotes Early expression of key genes in Bd21 starch biosynthesis mediate starch synthesis in the pericarp; intermediate-stage expression increases the number and size of starch granules.
T9 1072-1252 Sentence denotes In contrast, these enzymes in CS and Ae. peregrina were mostly expressed at intermediate stages, driving production of new B-granules and increasing the granule size, respectively.
T10 1253-1445 Sentence denotes Immunogold labeling showed that granule-bound starch synthase (GBSSI; related to amylose synthesis) was mainly present in starch granules: at lower levels in the B-granules of Bd21 than in CS.
T11 1446-1696 Sentence denotes Furthermore, GBSSI was phosphorylated at threonine 183 and tyrosine 185 in the starch synthase catalytic domain in CS and Ae. peregrina, but neither site was phosphorylated in Bd21, suggesting GBSSI phosphorylation could improve amylose biosynthesis.
T12 1697-1709 Sentence denotes CONCLUSIONS:
T13 1710-1863 Sentence denotes Bd21 contains only B-granules, and the expression of key genes in the three studied genera is consistent with the dynamic development of starch granules.
T14 1864-2065 Sentence denotes GBSSI is present in greater amounts in the B-granules of CS than in Bd21; two phosphorylation sites (Thr183 and Tyr185) were found in Triticum and Aegilops; these sites were not phosphorylated in Bd21.
T15 2066-2136 Sentence denotes GBSSI phosphorylation may reflect its importance in amylose synthesis.

Oryza_sentences

Id Subject Object Predicate Lexical cue
T1 0-161 Sentence denotes Dynamic development of starch granules and the regulation of starch biosynthesis in Brachypodium distachyon: comparison with common wheat and Aegilops peregrina.
T2 162-173 Sentence denotes BACKGROUND:
T3 174-324 Sentence denotes Thorough understanding of seed starch biosynthesis and accumulation mechanisms is of great importance for agriculture and crop improvement strategies.
T4 325-590 Sentence denotes We conducted the first comprehensive study of the dynamic development of starch granules and the regulation of starch biosynthesis in Brachypodium distachyon and compared the findings with those reported for common wheat (Chinese Spring, CS) and Aegilops peregrina.
T5 591-599 Sentence denotes RESULTS:
T6 600-759 Sentence denotes Only B-granules were identified in Brachypodium Bd21, and the shape variation and development of starch granules were similar in the B-granules of CS and Bd21.
T7 760-891 Sentence denotes Phylogenetic analysis showed that most of the Bd21 starch synthesis-related genes were more similar to those in wheat than in rice.
T8 892-1071 Sentence denotes Early expression of key genes in Bd21 starch biosynthesis mediate starch synthesis in the pericarp; intermediate-stage expression increases the number and size of starch granules.
T9 1072-1252 Sentence denotes In contrast, these enzymes in CS and Ae. peregrina were mostly expressed at intermediate stages, driving production of new B-granules and increasing the granule size, respectively.
T10 1253-1445 Sentence denotes Immunogold labeling showed that granule-bound starch synthase (GBSSI; related to amylose synthesis) was mainly present in starch granules: at lower levels in the B-granules of Bd21 than in CS.
T11 1446-1696 Sentence denotes Furthermore, GBSSI was phosphorylated at threonine 183 and tyrosine 185 in the starch synthase catalytic domain in CS and Ae. peregrina, but neither site was phosphorylated in Bd21, suggesting GBSSI phosphorylation could improve amylose biosynthesis.
T12 1697-1709 Sentence denotes CONCLUSIONS:
T13 1710-1863 Sentence denotes Bd21 contains only B-granules, and the expression of key genes in the three studied genera is consistent with the dynamic development of starch granules.
T14 1864-2065 Sentence denotes GBSSI is present in greater amounts in the B-granules of CS than in Bd21; two phosphorylation sites (Thr183 and Tyr185) were found in Triticum and Aegilops; these sites were not phosphorylated in Bd21.
T15 2066-2136 Sentence denotes GBSSI phosphorylation may reflect its importance in amylose synthesis.

Oryza_sentences

Id Subject Object Predicate Lexical cue
T1 0-161 Sentence denotes Dynamic development of starch granules and the regulation of starch biosynthesis in Brachypodium distachyon: comparison with common wheat and Aegilops peregrina.
T2 162-173 Sentence denotes BACKGROUND:
T3 174-324 Sentence denotes Thorough understanding of seed starch biosynthesis and accumulation mechanisms is of great importance for agriculture and crop improvement strategies.
T4 325-590 Sentence denotes We conducted the first comprehensive study of the dynamic development of starch granules and the regulation of starch biosynthesis in Brachypodium distachyon and compared the findings with those reported for common wheat (Chinese Spring, CS) and Aegilops peregrina.
T5 591-599 Sentence denotes RESULTS:
T6 600-759 Sentence denotes Only B-granules were identified in Brachypodium Bd21, and the shape variation and development of starch granules were similar in the B-granules of CS and Bd21.
T7 760-891 Sentence denotes Phylogenetic analysis showed that most of the Bd21 starch synthesis-related genes were more similar to those in wheat than in rice.
T8 892-1071 Sentence denotes Early expression of key genes in Bd21 starch biosynthesis mediate starch synthesis in the pericarp; intermediate-stage expression increases the number and size of starch granules.
T9 1072-1252 Sentence denotes In contrast, these enzymes in CS and Ae. peregrina were mostly expressed at intermediate stages, driving production of new B-granules and increasing the granule size, respectively.
T10 1253-1445 Sentence denotes Immunogold labeling showed that granule-bound starch synthase (GBSSI; related to amylose synthesis) was mainly present in starch granules: at lower levels in the B-granules of Bd21 than in CS.
T11 1446-1696 Sentence denotes Furthermore, GBSSI was phosphorylated at threonine 183 and tyrosine 185 in the starch synthase catalytic domain in CS and Ae. peregrina, but neither site was phosphorylated in Bd21, suggesting GBSSI phosphorylation could improve amylose biosynthesis.
T12 1697-1709 Sentence denotes CONCLUSIONS:
T13 1710-1863 Sentence denotes Bd21 contains only B-granules, and the expression of key genes in the three studied genera is consistent with the dynamic development of starch granules.
T14 1864-2065 Sentence denotes GBSSI is present in greater amounts in the B-granules of CS than in Bd21; two phosphorylation sites (Thr183 and Tyr185) were found in Triticum and Aegilops; these sites were not phosphorylated in Bd21.
T15 2066-2136 Sentence denotes GBSSI phosphorylation may reflect its importance in amylose synthesis.