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PubMed:2505925 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-92 Sentence denotes Purification of endo-N-acetyl-beta-D-glucosaminidase H by substrate-affinity chromatography.
TextSentencer_T2 93-230 Sentence denotes Endo-N-acetyl-beta-D-glucosaminidase H (Endo H) was purified to homogeneity (3000-fold) from a culture filtrate to Streptomyces plicatus.
TextSentencer_T3 231-393 Sentence denotes The key step was substrate-affinity chromatography, which afforded a 1000-fold purification and yielded a protease- and exoglycosidase-free preparation of Endo H.
TextSentencer_T4 394-562 Sentence denotes Proteins from the crude sample were applied to the substrate-affinity column, consisting of yeast-invertase glycopeptides bound to Sepharose-immobilized concanavalin A.
TextSentencer_T5 563-670 Sentence denotes After washing off the unbound proteins, Endo H was quantitatively eluted by methyl alpha-D-mannopyranoside.
TextSentencer_T6 671-773 Sentence denotes Various conditions were tested to achieve an optimal binding of Endo H to this substrate-affinity gel.
TextSentencer_T7 774-954 Sentence denotes After substrate-affinity chromatography, Endo H was separated from the coeluted gylcopeptide substrate and some protein impurities by gel filtration and hydrophobic chromatography.
T1 0-92 Sentence denotes Purification of endo-N-acetyl-beta-D-glucosaminidase H by substrate-affinity chromatography.
T2 93-230 Sentence denotes Endo-N-acetyl-beta-D-glucosaminidase H (Endo H) was purified to homogeneity (3000-fold) from a culture filtrate to Streptomyces plicatus.
T3 231-393 Sentence denotes The key step was substrate-affinity chromatography, which afforded a 1000-fold purification and yielded a protease- and exoglycosidase-free preparation of Endo H.
T4 394-562 Sentence denotes Proteins from the crude sample were applied to the substrate-affinity column, consisting of yeast-invertase glycopeptides bound to Sepharose-immobilized concanavalin A.
T5 563-670 Sentence denotes After washing off the unbound proteins, Endo H was quantitatively eluted by methyl alpha-D-mannopyranoside.
T6 671-773 Sentence denotes Various conditions were tested to achieve an optimal binding of Endo H to this substrate-affinity gel.
T7 774-954 Sentence denotes After substrate-affinity chromatography, Endo H was separated from the coeluted gylcopeptide substrate and some protein impurities by gel filtration and hydrophobic chromatography.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 208-229 OrganismTaxon denotes Streptomyces plicatus 1922