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PubMed:2485232 JSONTXT

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Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 28-33 http://purl.obolibrary.org/obo/MAT_0000098 denotes brain
T2 316-321 http://purl.obolibrary.org/obo/MAT_0000098 denotes brain

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-81 Sentence denotes Different reactivity of two brain sialyltransferases towards sulfhydryl reagents.
TextSentencer_T2 82-224 Sentence denotes Evidence for a thiol group involved in the nucleotide-sugar binding site of the NeuAc alpha 2-3Gal beta 1-3GalNAc alpha(2-6)sialyltransferase.
TextSentencer_T3 225-375 Sentence denotes We have studied the amino-acid residues involved in the catalytic activity of two distinct brain sialyltransferases acting on fetuin and asialofetuin.
TextSentencer_T4 376-493 Sentence denotes These two enzymes were strongly inhibited by N-bromosuccinimide, a specific blocking reagent for tryptophan residues.
TextSentencer_T5 494-603 Sentence denotes This result suggests the involvement of such residues in the catalytic process of the two sialyltransferases.
TextSentencer_T6 604-799 Sentence denotes Furthermore, chemical modifications by various sulfhydryl reagents led to a strong inhibition of the fetuin sialyltransferase while the asialofetuin sialyltransferase was only slightly inhibited.
TextSentencer_T7 800-1012 Sentence denotes For a more thorough understanding of the thiol inactivation mechanism of the fetuin sialyltransferase, we studied in more detail the reactivity of this enzyme with NEM (N-ethylmaleimide), an irreversible reagent.
TextSentencer_T8 1013-1175 Sentence denotes The time-dependent inactivation followed first-order kinetics and these kinetic data afforded presumptive evidence for the binding of 1 mol NEM per mol of enzyme.
TextSentencer_T9 1176-1249 Sentence denotes Only CMP-NeuAc protected the enzyme against NEM inactivation effectively.
TextSentencer_T10 1250-1307 Sentence denotes MnCl2 did not enhance the protective effect of CMP-NeuAc.
TextSentencer_T11 1308-1441 Sentence denotes The modifications of the fetuin sialyltransferase kinetic parameters by NEM showed a competitive mechanism between NEM and CMP-NeuAc.
TextSentencer_T12 1442-1786 Sentence denotes The results suggest the involvement of a sulfhydryl residue in or near the nucleotide-sugar binding site of the fetuin sialyltransferase (but we could not excluded that CMP-NeuAc binding may induce a change in conformation of the protein, leading to a decreased accessibility of this thiol group located near the nucleotide-sugar binding site).
TextSentencer_T13 1787-1899 Sentence denotes This SH group is essential to the enzyme activity, which is not the case for the asialofetuin sialyltransferase.
T1 0-81 Sentence denotes Different reactivity of two brain sialyltransferases towards sulfhydryl reagents.
T2 82-224 Sentence denotes Evidence for a thiol group involved in the nucleotide-sugar binding site of the NeuAc alpha 2-3Gal beta 1-3GalNAc alpha(2-6)sialyltransferase.
T3 225-375 Sentence denotes We have studied the amino-acid residues involved in the catalytic activity of two distinct brain sialyltransferases acting on fetuin and asialofetuin.
T4 376-493 Sentence denotes These two enzymes were strongly inhibited by N-bromosuccinimide, a specific blocking reagent for tryptophan residues.
T5 494-603 Sentence denotes This result suggests the involvement of such residues in the catalytic process of the two sialyltransferases.
T6 604-799 Sentence denotes Furthermore, chemical modifications by various sulfhydryl reagents led to a strong inhibition of the fetuin sialyltransferase while the asialofetuin sialyltransferase was only slightly inhibited.
T7 800-1012 Sentence denotes For a more thorough understanding of the thiol inactivation mechanism of the fetuin sialyltransferase, we studied in more detail the reactivity of this enzyme with NEM (N-ethylmaleimide), an irreversible reagent.
T8 1013-1175 Sentence denotes The time-dependent inactivation followed first-order kinetics and these kinetic data afforded presumptive evidence for the binding of 1 mol NEM per mol of enzyme.
T9 1176-1249 Sentence denotes Only CMP-NeuAc protected the enzyme against NEM inactivation effectively.
T10 1250-1307 Sentence denotes MnCl2 did not enhance the protective effect of CMP-NeuAc.
T11 1308-1441 Sentence denotes The modifications of the fetuin sialyltransferase kinetic parameters by NEM showed a competitive mechanism between NEM and CMP-NeuAc.
T12 1442-1786 Sentence denotes The results suggest the involvement of a sulfhydryl residue in or near the nucleotide-sugar binding site of the fetuin sialyltransferase (but we could not excluded that CMP-NeuAc binding may induce a change in conformation of the protein, leading to a decreased accessibility of this thiol group located near the nucleotide-sugar binding site).
T13 1787-1899 Sentence denotes This SH group is essential to the enzyme activity, which is not the case for the asialofetuin sialyltransferase.

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 162-167 Glycan denotes NeuAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T2 1185-1190 Glycan denotes NeuAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T3 1301-1306 Glycan denotes NeuAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T4 1435-1440 Glycan denotes NeuAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T5 1615-1620 Glycan denotes NeuAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 28-33 Body_part denotes brain http://purl.obolibrary.org/obo/MAT_0000098
T2 316-321 Body_part denotes brain http://purl.obolibrary.org/obo/MAT_0000098

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 162-167 Glycan denotes NeuAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T2 1185-1190 Glycan denotes NeuAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T3 1301-1306 Glycan denotes NeuAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T4 1435-1440 Glycan denotes NeuAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR
T5 1615-1620 Glycan denotes NeuAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR

GlyCosmos15-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 28-33 Body_part denotes brain http://purl.obolibrary.org/obo/UBERON_0000955
T2 316-321 Body_part denotes brain http://purl.obolibrary.org/obo/UBERON_0000955

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-81 Sentence denotes Different reactivity of two brain sialyltransferases towards sulfhydryl reagents.
T2 82-224 Sentence denotes Evidence for a thiol group involved in the nucleotide-sugar binding site of the NeuAc alpha 2-3Gal beta 1-3GalNAc alpha(2-6)sialyltransferase.
T3 225-375 Sentence denotes We have studied the amino-acid residues involved in the catalytic activity of two distinct brain sialyltransferases acting on fetuin and asialofetuin.
T4 376-493 Sentence denotes These two enzymes were strongly inhibited by N-bromosuccinimide, a specific blocking reagent for tryptophan residues.
T5 494-603 Sentence denotes This result suggests the involvement of such residues in the catalytic process of the two sialyltransferases.
T6 604-799 Sentence denotes Furthermore, chemical modifications by various sulfhydryl reagents led to a strong inhibition of the fetuin sialyltransferase while the asialofetuin sialyltransferase was only slightly inhibited.
T7 800-1012 Sentence denotes For a more thorough understanding of the thiol inactivation mechanism of the fetuin sialyltransferase, we studied in more detail the reactivity of this enzyme with NEM (N-ethylmaleimide), an irreversible reagent.
T8 1013-1175 Sentence denotes The time-dependent inactivation followed first-order kinetics and these kinetic data afforded presumptive evidence for the binding of 1 mol NEM per mol of enzyme.
T9 1176-1249 Sentence denotes Only CMP-NeuAc protected the enzyme against NEM inactivation effectively.
T10 1250-1307 Sentence denotes MnCl2 did not enhance the protective effect of CMP-NeuAc.
T11 1308-1441 Sentence denotes The modifications of the fetuin sialyltransferase kinetic parameters by NEM showed a competitive mechanism between NEM and CMP-NeuAc.
T12 1442-1786 Sentence denotes The results suggest the involvement of a sulfhydryl residue in or near the nucleotide-sugar binding site of the fetuin sialyltransferase (but we could not excluded that CMP-NeuAc binding may induce a change in conformation of the protein, leading to a decreased accessibility of this thiol group located near the nucleotide-sugar binding site).
T13 1787-1899 Sentence denotes This SH group is essential to the enzyme activity, which is not the case for the asialofetuin sialyltransferase.

GlyCosmos15-FMA

Id Subject Object Predicate Lexical cue db_id
T1 28-33 Body_part denotes brain FMA:50801
T2 316-321 Body_part denotes brain FMA:50801

GlyCosmos15-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 28-33 Body_part denotes brain http://purl.obolibrary.org/obo/MAT_0000098
T2 316-321 Body_part denotes brain http://purl.obolibrary.org/obo/MAT_0000098

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 28-33 Body_part denotes brain http://purl.obolibrary.org/obo/UBERON_0000955|http://purl.obolibrary.org/obo/UBERON_6110636
T3 316-321 Body_part denotes brain http://purl.obolibrary.org/obo/UBERON_0000955|http://purl.obolibrary.org/obo/UBERON_6110636