PubMed:24802396 JSONTXT

{"project":"Allie","denotations":[{"id":"SS1_24802396_1_0","span":{"begin":86,"end":100},"obj":"expanded"},{"id":"SS2_24802396_1_0","span":{"begin":102,"end":104},"obj":"abbr"},{"id":"SS1_24802396_4_0","span":{"begin":603,"end":627},"obj":"expanded"},{"id":"SS2_24802396_4_0","span":{"begin":629,"end":634},"obj":"abbr"}],"relations":[{"id":"AE1_24802396_1_0","pred":"abbreviatedTo","subj":"SS1_24802396_1_0","obj":"SS2_24802396_1_0"},{"id":"AE1_24802396_4_0","pred":"abbreviatedTo","subj":"SS1_24802396_4_0","obj":"SS2_24802396_4_0"}],"text":"Glutathione peroxidase-1 is required for self-renewal of murine embryonic stem cells.\nEmbryonic stem (ES) cells are pluripotent cells that are capable of giving rise to any type of cells in the body and possess unlimited self-renewal potential. However, the exact regulatory mechanisms that govern the self-renewal ability of ES cells remain elusive. To understand the immediate early events during ES cell differentiation, we performed a proteomics study and analyzed the proteomic difference in murine ES cells before and after a 6-h spontaneous differentiation. We found that the expression level of glutathione peroxidase-1 (GPx-1), an antioxidant enzyme, is dramatically decreased upon the differentiation. Both knockdown of GPx-1 expression with shRNA and inhibiting GPx-1 activity by inhibitor led to the differentiation of ES cells. Furthermore, we showed that during early differentiation, the quick degradation of GPx-1 was mediated by proteasome. Thus, our data indicated that GPx-1 is a key regulator of self-renewal of murine embryonic stem cells."}