| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-108 |
Sentence |
denotes |
Vitamin D3 metabolites modulate dihydropyridine-sensitive calcium currents in clonal rat osteosarcoma cells. |
| T2 |
109-282 |
Sentence |
denotes |
A slowly inactivating inward calcium current was identified in the rat osteosarcoma cell line ROS 17/2.8 using a combination of ion flux and electrophysiological techniques. |
| T3 |
283-461 |
Sentence |
denotes |
Voltage dependence, dihydropyridine sensitivity, divalent cation selectivity, and single channel properties identified this current as a high threshold, "L-type" calcium current. |
| T4 |
462-617 |
Sentence |
denotes |
Ion flux experiments using 45Ca2+ confirmed that calcium uptake through these channel represents a major pathway for calcium entry into osteosarcoma cells. |
| T5 |
618-816 |
Sentence |
denotes |
In resting cells, i.e. at negative membrane potentials, stimulation of both calcium current and rapid 45Ca2+ influx could be elicited by concentrations of 1,25-(OH)2-vitamin D3 between 0.1 and 3 nM. |
| T6 |
817-955 |
Sentence |
denotes |
At these concentrations, 1,25-(OH)2-vitamin D3 shifted the threshold for activation of inward calcium current to more negative potentials. |
| T7 |
956-1030 |
Sentence |
denotes |
At higher concentrations (5-10 nM), inhibitory effects became predominant. |
| T8 |
1031-1122 |
Sentence |
denotes |
These opposing effects are functionally similar to those of the dihydropyridine BAY K 8644. |
| T9 |
1123-1287 |
Sentence |
denotes |
Other vitamin D3 metabolites (25-(OH)-D3 and 24,25-(OH)2-D3) exhibited less potent stimulatory effects and greater inhibition of calcium current than 1,25-(OH)2-D3. |
| T10 |
1288-1548 |
Sentence |
denotes |
These results suggest that (i) vitamin D3 acts as a potent modulator of calcium channel function in osteosarcoma cells, and (ii) intracellular Ca2+-dependent signaling processes may be affected acutely by physiological concentrations of vitamin D3 metabolites. |
| T1 |
0-108 |
Sentence |
denotes |
Vitamin D3 metabolites modulate dihydropyridine-sensitive calcium currents in clonal rat osteosarcoma cells. |
| T2 |
109-282 |
Sentence |
denotes |
A slowly inactivating inward calcium current was identified in the rat osteosarcoma cell line ROS 17/2.8 using a combination of ion flux and electrophysiological techniques. |
| T3 |
283-461 |
Sentence |
denotes |
Voltage dependence, dihydropyridine sensitivity, divalent cation selectivity, and single channel properties identified this current as a high threshold, "L-type" calcium current. |
| T4 |
462-617 |
Sentence |
denotes |
Ion flux experiments using 45Ca2+ confirmed that calcium uptake through these channel represents a major pathway for calcium entry into osteosarcoma cells. |
| T5 |
618-816 |
Sentence |
denotes |
In resting cells, i.e. at negative membrane potentials, stimulation of both calcium current and rapid 45Ca2+ influx could be elicited by concentrations of 1,25-(OH)2-vitamin D3 between 0.1 and 3 nM. |
| T6 |
817-955 |
Sentence |
denotes |
At these concentrations, 1,25-(OH)2-vitamin D3 shifted the threshold for activation of inward calcium current to more negative potentials. |
| T7 |
956-1030 |
Sentence |
denotes |
At higher concentrations (5-10 nM), inhibitory effects became predominant. |
| T8 |
1031-1122 |
Sentence |
denotes |
These opposing effects are functionally similar to those of the dihydropyridine BAY K 8644. |
| T9 |
1123-1287 |
Sentence |
denotes |
Other vitamin D3 metabolites (25-(OH)-D3 and 24,25-(OH)2-D3) exhibited less potent stimulatory effects and greater inhibition of calcium current than 1,25-(OH)2-D3. |
| T10 |
1288-1548 |
Sentence |
denotes |
These results suggest that (i) vitamin D3 acts as a potent modulator of calcium channel function in osteosarcoma cells, and (ii) intracellular Ca2+-dependent signaling processes may be affected acutely by physiological concentrations of vitamin D3 metabolites. |