| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-190 |
Sentence |
denotes |
Targeting cell migration and the endoplasmic reticulum stress response with calmodulin antagonists: a clinically tested small molecule phenocopy of SEC62 gene silencing in human tumor cells. |
| TextSentencer_T2 |
191-202 |
Sentence |
denotes |
BACKGROUND: |
| TextSentencer_T3 |
203-286 |
Sentence |
denotes |
Tumor cells benefit from their ability to avoid apoptosis and invade other tissues. |
| TextSentencer_T4 |
287-376 |
Sentence |
denotes |
The endoplasmic reticulum (ER) membrane protein Sec62 is a key player in these processes. |
| TextSentencer_T5 |
377-520 |
Sentence |
denotes |
Sec62 is essential for cell migration and protects tumor cells against thapsigargin-induced ER stress, which are both linked to cytosolic Ca²⁺. |
| TextSentencer_T6 |
521-629 |
Sentence |
denotes |
SEC62 silencing leads to elevated cytosolic Ca²⁺ and increased ER Ca²⁺ leakage after thapsigargin treatment. |
| TextSentencer_T7 |
630-744 |
Sentence |
denotes |
Sec62 protein levels are significantly increased in different tumors, including prostate, lung and thyroid cancer. |
| TextSentencer_T8 |
745-753 |
Sentence |
denotes |
METHODS: |
| TextSentencer_T9 |
754-889 |
Sentence |
denotes |
In lung cancer, the influence of Sec62 protein levels on patient survival was analyzed using the Kaplan-Meier method and log-rank test. |
| TextSentencer_T10 |
890-1047 |
Sentence |
denotes |
To elucidate the underlying pathophysiological functions of Sec62, Ca²⁺ imaging techniques, real-time cell analysis and cell migration assays were performed. |
| TextSentencer_T11 |
1048-1326 |
Sentence |
denotes |
The effects of treatment with the calmodulin antagonists, trifluoperazine (TFP) and ophiobolin A, on cellular Ca²⁺ homeostasis, cell growth and cell migration were compared with the effects of siRNA-mediated Sec62 depletion or the expression of a mutated SEC62 variant in vitro. |
| TextSentencer_T12 |
1327-1425 |
Sentence |
denotes |
Using Biacore analysis we examined the Ca²⁺-sensitive interaction of Sec62 with the Sec61 complex. |
| TextSentencer_T13 |
1426-1434 |
Sentence |
denotes |
RESULTS: |
| TextSentencer_T14 |
1435-1511 |
Sentence |
denotes |
Sec62 overproduction significantly correlated with reduced patient survival. |
| TextSentencer_T15 |
1512-1628 |
Sentence |
denotes |
Therefore, Sec62 is not only a predictive marker for this type of tumor, but also an interesting therapeutic target. |
| TextSentencer_T16 |
1629-1783 |
Sentence |
denotes |
The present study suggests a regulatory function for Sec62 in the major Ca²⁺ leakage channel in the ER, Sec61, by a direct and Ca²⁺-sensitive interaction. |
| TextSentencer_T17 |
1784-1854 |
Sentence |
denotes |
A Ca²⁺-binding motif in Sec62 is essential for its molecular function. |
| TextSentencer_T18 |
1855-2016 |
Sentence |
denotes |
Treatment of cells with calmodulin antagonists mimicked Sec62 depletion by inhibiting cell migration and rendering the cells sensitive to thapsigargin treatment. |
| TextSentencer_T19 |
2017-2029 |
Sentence |
denotes |
CONCLUSIONS: |
| TextSentencer_T20 |
2030-2199 |
Sentence |
denotes |
Targeting tumors that overproduce Sec62 with calmodulin antagonists in combination with targeted thapsigargin analogues may offer novel personalized therapeutic options. |
| T1 |
0-190 |
Sentence |
denotes |
Targeting cell migration and the endoplasmic reticulum stress response with calmodulin antagonists: a clinically tested small molecule phenocopy of SEC62 gene silencing in human tumor cells. |
| T2 |
191-202 |
Sentence |
denotes |
BACKGROUND: |
| T3 |
203-286 |
Sentence |
denotes |
Tumor cells benefit from their ability to avoid apoptosis and invade other tissues. |
| T4 |
287-376 |
Sentence |
denotes |
The endoplasmic reticulum (ER) membrane protein Sec62 is a key player in these processes. |
| T5 |
377-520 |
Sentence |
denotes |
Sec62 is essential for cell migration and protects tumor cells against thapsigargin-induced ER stress, which are both linked to cytosolic Ca²⁺. |
| T6 |
521-629 |
Sentence |
denotes |
SEC62 silencing leads to elevated cytosolic Ca²⁺ and increased ER Ca²⁺ leakage after thapsigargin treatment. |
| T7 |
630-744 |
Sentence |
denotes |
Sec62 protein levels are significantly increased in different tumors, including prostate, lung and thyroid cancer. |
| T8 |
745-753 |
Sentence |
denotes |
METHODS: |
| T9 |
754-889 |
Sentence |
denotes |
In lung cancer, the influence of Sec62 protein levels on patient survival was analyzed using the Kaplan-Meier method and log-rank test. |
| T10 |
890-1047 |
Sentence |
denotes |
To elucidate the underlying pathophysiological functions of Sec62, Ca²⁺ imaging techniques, real-time cell analysis and cell migration assays were performed. |
| T11 |
1048-1326 |
Sentence |
denotes |
The effects of treatment with the calmodulin antagonists, trifluoperazine (TFP) and ophiobolin A, on cellular Ca²⁺ homeostasis, cell growth and cell migration were compared with the effects of siRNA-mediated Sec62 depletion or the expression of a mutated SEC62 variant in vitro. |
| T12 |
1327-1425 |
Sentence |
denotes |
Using Biacore analysis we examined the Ca²⁺-sensitive interaction of Sec62 with the Sec61 complex. |
| T13 |
1426-1434 |
Sentence |
denotes |
RESULTS: |
| T14 |
1435-1511 |
Sentence |
denotes |
Sec62 overproduction significantly correlated with reduced patient survival. |
| T15 |
1512-1628 |
Sentence |
denotes |
Therefore, Sec62 is not only a predictive marker for this type of tumor, but also an interesting therapeutic target. |
| T16 |
1629-1783 |
Sentence |
denotes |
The present study suggests a regulatory function for Sec62 in the major Ca²⁺ leakage channel in the ER, Sec61, by a direct and Ca²⁺-sensitive interaction. |
| T17 |
1784-1854 |
Sentence |
denotes |
A Ca²⁺-binding motif in Sec62 is essential for its molecular function. |
| T18 |
1855-2016 |
Sentence |
denotes |
Treatment of cells with calmodulin antagonists mimicked Sec62 depletion by inhibiting cell migration and rendering the cells sensitive to thapsigargin treatment. |
| T19 |
2017-2029 |
Sentence |
denotes |
CONCLUSIONS: |
| T20 |
2030-2199 |
Sentence |
denotes |
Targeting tumors that overproduce Sec62 with calmodulin antagonists in combination with targeted thapsigargin analogues may offer novel personalized therapeutic options. |