PubMed:24269685 JSONTXT

{"project":"Allie","denotations":[{"id":"SS1_24269685_1_0","span":{"begin":155,"end":165},"obj":"expanded"},{"id":"SS2_24269685_1_0","span":{"begin":167,"end":169},"obj":"abbr"}],"relations":[{"id":"AE1_24269685_1_0","pred":"abbreviatedTo","subj":"SS1_24269685_1_0","obj":"SS2_24269685_1_0"}],"text":"N-Terminal signal sequence is required for cellular trafficking and hyaluronan-depolymerization of KIAA1199.\nRecently, we disclosed that KIAA1199-mediated hyaluronan (HA) depolymerization requires an acidic cellular microenvironment (e.g. clathrin-coated vesicles or early endosomes), but no information about the structural basis underlying the cellular targeting and functional modification of KIAA1199 was available. Here, we show that the cleavage of N-terminal 30 amino acids occurs in functionally matured KIAA1199, and the deletion of the N-terminal portion results in altered intracellular trafficking of the molecule and loss of cellular HA depolymerization. These results suggest that the N-terminal portion of KIAA1199 functions as a cleavable signal sequence required for proper KIAA1199 translocation and KIAA1199-mediated HA depolymerization."}