PubMed:23624934 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/23624934","sourcedb":"PubMed","sourceid":"23624934","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/23624934","text":"Arginine methylation of the c-Jun coactivator RACO-1 is required for c-Jun/AP-1 activation.\nc-Jun, the major component of the AP-1 transcription factor complex, has important functions in cellular proliferation and oncogenic transformation. The RING domain-containing protein RACO-1 functions as a c-Jun coactivator that molecularly links growth factor signalling to AP-1 transactivation. Here we demonstrate that RACO-1 is present as a nuclear dimer and that c-Jun specifically interacts with dimeric RACO-1. Moreover, RACO-1 is identified as a substrate of the arginine methyltransferase PRMT1, which methylates RACO-1 on two arginine residues. Arginine methylation of RACO-1 promotes a conformational change that stabilises RACO-1 by facilitating K63-linked ubiquitin chain formation, and enables RACO-1 dimerisation and c-Jun interaction. Abrogation of PRMT1 function impairs AP-1 activity and results in decreased expression of a large percentage of c-Jun target genes. These results demonstrate that arginine methylation of RACO-1 is required for efficient transcriptional activation by c-Jun/AP-1 and thus identify PRMT1 as an important regulator of c-Jun/AP-1 function.","tracks":[{"project":"relna","denotations":[{"id":"T1","span":{"begin":28,"end":33},"obj":"DNA"},{"id":"T2","span":{"begin":46,"end":52},"obj":"Protein"},{"id":"T3","span":{"begin":69,"end":74},"obj":"DNA"},{"id":"T4","span":{"begin":92,"end":97},"obj":"DNA"},{"id":"T5","span":{"begin":276,"end":282},"obj":"Protein"},{"id":"T6","span":{"begin":298,"end":303},"obj":"DNA"},{"id":"T7","span":{"begin":414,"end":420},"obj":"Protein"},{"id":"T8","span":{"begin":460,"end":465},"obj":"DNA"},{"id":"T9","span":{"begin":502,"end":508},"obj":"Protein"},{"id":"T10","span":{"begin":520,"end":526},"obj":"Protein"},{"id":"T11","span":{"begin":590,"end":595},"obj":"Protein"},{"id":"T12","span":{"begin":614,"end":620},"obj":"Protein"},{"id":"T13","span":{"begin":671,"end":677},"obj":"Protein"},{"id":"T14","span":{"begin":727,"end":733},"obj":"Protein"},{"id":"T15","span":{"begin":800,"end":806},"obj":"Protein"},{"id":"T16","span":{"begin":824,"end":829},"obj":"DNA"},{"id":"T17","span":{"begin":857,"end":862},"obj":"Protein"},{"id":"T18","span":{"begin":955,"end":960},"obj":"DNA"},{"id":"T19","span":{"begin":1030,"end":1036},"obj":"Protein"},{"id":"T20","span":{"begin":1093,"end":1098},"obj":"DNA"},{"id":"T21","span":{"begin":1122,"end":1127},"obj":"Protein"},{"id":"T22","span":{"begin":1157,"end":1162},"obj":"DNA"}],"relations":[{"id":"R0","pred":"linked","subj":"T2","obj":"T1"},{"id":"R1","pred":"linked","subj":"T5","obj":"T6"},{"id":"R2","pred":"linked","subj":"T9","obj":"T8"},{"id":"R3","pred":"linked","subj":"T15","obj":"T16"}],"attributes":[{"subj":"T1","pred":"source","obj":"relna"},{"subj":"T2","pred":"source","obj":"relna"},{"subj":"T3","pred":"source","obj":"relna"},{"subj":"T4","pred":"source","obj":"relna"},{"subj":"T5","pred":"source","obj":"relna"},{"subj":"T6","pred":"source","obj":"relna"},{"subj":"T7","pred":"source","obj":"relna"},{"subj":"T8","pred":"source","obj":"relna"},{"subj":"T9","pred":"source","obj":"relna"},{"subj":"T10","pred":"source","obj":"relna"},{"subj":"T11","pred":"source","obj":"relna"},{"subj":"T12","pred":"source","obj":"relna"},{"subj":"T13","pred":"source","obj":"relna"},{"subj":"T14","pred":"source","obj":"relna"},{"subj":"T15","pred":"source","obj":"relna"},{"subj":"T16","pred":"source","obj":"relna"},{"subj":"T17","pred":"source","obj":"relna"},{"subj":"T18","pred":"source","obj":"relna"},{"subj":"T19","pred":"source","obj":"relna"},{"subj":"T20","pred":"source","obj":"relna"},{"subj":"T21","pred":"source","obj":"relna"},{"subj":"T22","pred":"source","obj":"relna"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"relna","color":"#ecd693","default":true}]}]}}