PubMed:23603051 JSONTXT

{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T1","span":{"begin":114,"end":275},"obj":"BACKGROUND"},{"id":"T2","span":{"begin":288,"end":403},"obj":"OBJECTIVE"},{"id":"T3","span":{"begin":413,"end":688},"obj":"METHODS"},{"id":"T4","span":{"begin":698,"end":929},"obj":"RESULTS"},{"id":"T5","span":{"begin":943,"end":1056},"obj":"CONCLUSIONS"}],"text":"A C. elegans screening platform for the rapid assessment of chemical disruption of germline function.\nBACKGROUND: Despite the developmental impact of chromosome segregation errors, we lack the tools to assess environmental effects on the integrity of the germline in animals.\nOBJECTIVES: We developed an assay in Caenorhabditis elegans that fluorescently marks aneuploid embryos after chemical exposure.\nMETHODS: We qualified the predictive value of the assay against chemotherapeutic agents as well as environmental compounds from the ToxCast Phase I library by comparing results from the C. elegans assay with the comprehensive mammalian in vivo end point data from the ToxRef database.\nRESULTS: The assay was highly predictive of mammalian reproductive toxicities, with a 69% maximum balanced accuracy. We confirmed the effect of select compounds on germline integrity by monitoring germline apoptosis and meiotic progression.\nCONCLUSIONS: This C. elegans assay provides a comprehensive strategy for assessing environmental effects on germline function."}