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PubMed:23589369 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-169 Sentence denotes Crystal and solution studies reveal that the transcriptional regulator AcnR of Corynebacterium glutamicum is regulated by citrate-Mg2+ binding to a non-canonical pocket.
T2 170-378 Sentence denotes Corynebacterium glutamicum is an important industrial bacterium as well as a model organism for the order Corynebacteriales, whose citric acid cycle occupies a central position in energy and precursor supply.
T3 379-596 Sentence denotes Expression of aconitase, which isomerizes citrate into isocitrate, is controlled by several transcriptional regulators, including the dimeric aconitase repressor AcnR, assigned by sequence identity to the TetR family.
T4 597-712 Sentence denotes We report the structures of AcnR in two crystal forms together with ligand binding experiments and in vivo studies.
T5 713-891 Sentence denotes First, there is a citrate-Mg(2+) moiety bound in both forms, not in the canonical TetR ligand binding site but rather in a second pocket more distant from the DNA binding domain.
T6 892-991 Sentence denotes Second, the citrate-Mg(2+) binds with a KD of 6 mM, within the range of physiological significance.
T7 992-1070 Sentence denotes Third, citrate-Mg(2+) lowers the affinity of AcnR for its target DNA in vitro.
T8 1071-1248 Sentence denotes Fourth, analyses of several AcnR point mutations provide evidence for the possible involvement of the corresponding residues in ligand binding, DNA binding, and signal transfer.
T9 1249-1354 Sentence denotes AcnR derivatives defective in citrate-Mg(2+) binding severely inhibit growth of C. glutamicum on citrate.
T10 1355-1648 Sentence denotes Finally, the structures do have a pocket corresponding to the canonical tetracycline site, and although we have not identified a ligand that binds there, comparison of the two crystal forms suggests differences in the region of the canonical pocket that may indicate a biological significance.

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 541-545 HP_0001061 denotes AcnR
T2 625-629 HP_0001061 denotes AcnR
T3 1037-1041 HP_0001061 denotes AcnR
T4 1099-1103 HP_0001061 denotes AcnR
T5 1249-1253 HP_0001061 denotes AcnR

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 79-105 OrganismTaxon denotes Corynebacterium glutamicum 1718
T2 170-196 OrganismTaxon denotes Corynebacterium glutamicum 1718
T3 276-293 OrganismTaxon denotes Corynebacteriales 85007

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 253-261 Body_part denotes organism http://purl.obolibrary.org/obo/UBERON_0000468