PubMed:23493052 / 579-1041 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/23493052","sourcedb":"PubMed","sourceid":"23493052","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/23493052","text":"The coding sequences of cytoplasmic and nuclear variants of lacZ gene were inserted downstream of the human cytomegalovirus immediate-early gene promoter of plasmid pcDNA3.1/myc-His C. The new cytoplasmic and nuclear constricts of E. coli β-galactosidase-coding sequences were introduced into HeLa cells with the aid of linear polyethylenimine and at 2 days post-transfection the cells were stained using 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal).","tracks":[{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T2","span":{"begin":0,"end":462},"obj":"METHODS"}],"attributes":[{"subj":"T2","pred":"source","obj":"PubMed_Structured_Abstracts"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubMed_Structured_Abstracts","color":"#93dfec","default":true}]}]}}