PubMed:2346933
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/2346933","sourcedb":"PubMed","sourceid":"2346933","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/2346933","text":"Further structural studies of anti-complementary acidic heteroglycans from the leaves of Panax ginseng C.A. Meyer.\nThe three potent anti-complementary polysaccharides, GL-PI, GL-PII, and GL-PIV, isolated from the leaves of Panax ginseng C. A. Meyer, were subjected to base-catalysed beta-elimination in the presence of sodium borodeuteride or enzymic digestion with endo-alpha-D-(1----4)-polygalacturonase. beta-Eliminative degradation of GL-PI and GL-PII each gave neutral (IN and IIN) and acidic (IA and IIA) fractions. Each fraction N consisted of Ara, Rha, Gal, and Glc, whereas each fraction A comprised a large proportion of GalA in addition to Rha, Gal, Glc, and GLcA. Methylation analysis and g.l.c.-m.s. showed that each fraction IN and IIN contained Rha-(1----2)-Rha-ol-1-d, Rha-(1----4)-Rha-ol-1-d, Ara-(1----4)-Rha-ol-1-d, Gal-(1----4)-Rha-ol-1-d, Gal-(1----6)-Gal-ol-1-d, and GlcA-(1----4)-Rha-ol-1-d, and that IA and IIA contained Rha----Rha-ol-1-d, HexA----Rha-ol-1-d, and HexA----Rha----Rha-ol-1-d. Methylation analysis indicated that IN and IIN also contained high-molecular-weight 6-linked galactan and 4-linked glucan, and that IA and IIA consisted mainly of 2-linked Rha, 4-linked GalA, and terminal and 6-linked Gal. IIA contained more 2-linked Rha than IA. Endo-alpha-D-(1----4)-polygalacturonase-mediated digestion of GL-PIV produced a high-molecular-weight fraction (PG-1) which was rich in neutral sugars, fragments of intermediate size (PG-2), and oligosaccharides (PG-3). PG-1 contained a rhamnogalacturonan core, galactan (which mainly comprised terminal, 6-linked, and 4,6-disubstituted Gal), and 4-linked glucans. PG-2 contained (1----4)-linked alpha-galacturonan partially branched at position 2 or 3 and a rhamnogalacturonan core in addition to small proportions of Gal and Glc. PG-3 contained large proportions of oligogalacturonides.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":107},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":108,"end":114},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":115,"end":239},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":240,"end":242},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":243,"end":521},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":522,"end":675},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":676,"end":1014},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1015,"end":1237},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1238,"end":1278},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1279,"end":1498},"obj":"Sentence"},{"id":"TextSentencer_T11","span":{"begin":1499,"end":1643},"obj":"Sentence"},{"id":"TextSentencer_T12","span":{"begin":1644,"end":1810},"obj":"Sentence"},{"id":"TextSentencer_T13","span":{"begin":1811,"end":1867},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":107},"obj":"Sentence"},{"id":"T2","span":{"begin":108,"end":114},"obj":"Sentence"},{"id":"T3","span":{"begin":115,"end":239},"obj":"Sentence"},{"id":"T4","span":{"begin":240,"end":242},"obj":"Sentence"},{"id":"T5","span":{"begin":243,"end":521},"obj":"Sentence"},{"id":"T6","span":{"begin":522,"end":675},"obj":"Sentence"},{"id":"T7","span":{"begin":676,"end":1014},"obj":"Sentence"},{"id":"T8","span":{"begin":1015,"end":1237},"obj":"Sentence"},{"id":"T9","span":{"begin":1238,"end":1278},"obj":"Sentence"},{"id":"T10","span":{"begin":1279,"end":1498},"obj":"Sentence"},{"id":"T11","span":{"begin":1499,"end":1643},"obj":"Sentence"},{"id":"T12","span":{"begin":1644,"end":1810},"obj":"Sentence"},{"id":"T13","span":{"begin":1811,"end":1867},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T9","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T10","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T11","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T12","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T13","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"},{"subj":"T11","pred":"source","obj":"sentences"},{"subj":"T12","pred":"source","obj":"sentences"},{"subj":"T13","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":89,"end":102},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":223,"end":236},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":551,"end":554},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":810,"end":813},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"4054"},{"id":"A2","pred":"db_id","subj":"T2","obj":"4054"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9225"},{"id":"A4","pred":"db_id","subj":"T4","obj":"9225"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"},{"subj":"T3","pred":"source","obj":"NCBITAXON"},{"subj":"T4","pred":"source","obj":"NCBITAXON"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":588,"end":598},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL_0002045"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"}]},{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":588,"end":598},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0002045"},{"subj":"T1","pred":"source","obj":"CL-cell"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#c793ec","default":true},{"id":"NCBITAXON","color":"#93ecad"},{"id":"Anatomy-UBERON","color":"#ec9393"},{"id":"CL-cell","color":"#93adec"}]}]}}